nm23-H1转染对肺癌细胞钙调素和基质金属蛋白酶表达的影响

目的:探讨nm23-H1转染对肺癌细胞钙调素和基质金属蛋白酶表达的影响.方法:利用半定量RT-PCR技术检测转染前后E-cadherin、MMP-9基因的mRNA表达;利用流式细胞仪技术检测转染前后E-cadherin、MMP-9基因的蛋白表达.结果:转染后细胞株E-cadherin mRNA与蛋白表达上调,转染后细胞株MMP-9 mRNA与蛋白表达下调.结论:nm23-H1基因对肺癌细胞株L9981 E-cadherin mRNA与蛋白表达具有正调控作用,对MMP-9 mRNA与蛋白表达具有负调控作用.     

Ahmed阀植入联合23G玻璃体切除治疗伴玻璃体积血的新生血管性青光眼

目的 评价Ahmed青光眼引流阀植入联合23G玻璃体切除术治疗伴玻璃体积血的新生血管性青光眼的效果.方法 对25例(25眼)伴玻璃体积血的新生血管性青光眼采用Ahmed阀植入联合23G玻璃体切除术治疗,统计分析术后不同时间眼压、手术成功率、最佳矫正视力及并发症,术后随访6 ～12个月,平均(9.96±1.93)月.结果 术后1d、1周、1个月、3个月和最后一次随访时平均眼压分别为(15.94±6.05)、(14.76±2.39)、(17.12±6.06)、(17.44±2.75)及(18.96±3.56) mmHg(1 mmHg=0.133 kPa).与术前平均眼压(48.12±7.29)mmHg比较,差异有统计学意义(t=16.733、21.961、16.917、17.609及18.091,P＜0.05);最后一次随访时手术总成功率为84.0％.所有患者视力均有提高.结论 Ahmed阀植入联合23G玻璃体切除术是治疗伴玻璃体积血的新生血管性青光眼比较有效的方法.     

糖尿病视网膜病变23G和20G玻璃体手术

目的 对比观察23G和20G玻璃体手术治疗增生型糖尿病视网膜病变（PDR）的临床结果和并发症.方法 接受玻璃体切除手术治疗的PDR 52例（64只眼）纳入本回顾性对比研究.按其所接受的手术方式分为23G微创玻璃体切除手术组（23G组）和20G玻璃体切除手术组（20G组）,分别为32例（40只眼）和20例（24只眼）.手术后随访6个月,对比分析两组的最佳矫正视力,眼压及术中术后并发症情况.结果 两组术后1,3和6个月最佳矫正视力均比术前有显著提高（χ2=20.32,22.56,18.23,P＜0.01）.23G组术后第一天的眼压低于术前眼压（t=2.75,P＜0.01）.23G组的手术时间短于20G组（t=2.71,P=0.01）.除23G组有9只眼（22.5％）发生早期术后低眼压（与20G组比较,χ2 =4.56,P=0.03）,两组术中术后并发症情况无明显差异.结论 23G微创玻璃体切除手术治疗PDR的有效性与20G玻璃体切除手术基本相同,两者术中术后并发症情况差异无统计学意义.     

Surgical outcomes of 23-gauge vitrectomy for the management of lens fragments dropped into the vitreous cavity during cataract surgery

Purpose

To assess the clinical features and surgical outcomes of 23-Gauge (G) vitrectomy for lens fragments dropped into the vitreous during cataract surgery.

Methods

A retrospective, non-comparative, interventional case series at a single medical center. The medical records of 45 eyes from 45 consecutive patients who were referred to our hospital for surgical retrieval of phacoemulsification dropped lens fragments and who underwent 23-G vitrectomy were retrospectively reviewed. Data pertaining to patient demographics, pre- and post-operative Snellen visual acuity, and postoperative complications were recorded. Factors associated with dropped lens fragments were also examined.

Results

Mean patient age was 68.18 ± 11.47 years. The preoperative and postoperative mean logarithm of minimum angle of resolution (logMAR) visual acuity was 1.91 ± 0.59 (Snellen equivalent 0.06 ± 0.15) and 0.42 ± 0.51 (Snellen equivalent 0.54 ± 0.31), respectively. Forty-two eyes (93.3%) had dislocated lens fragments <50% of the total lens size. Two eyes (4.4%) had a large and hard lens nucleus, which necessitated the use of a 20-G fragmatome to efficiently and completely remove the lens material. At the final examination, 30 eyes (66.6%) had a visual acuity better than 20/40. Post-vitrectomy complications included elevated IOP for at least 3 months (n = 5 eyes, 11.1%), intraocular lens dislocation (n = 2 eyes, 4.4%), and cystoid macular edema (n = 1 eye, 2.2%). No cases of postoperative endophthalmitis or retinal detachment were observed.

Conclusions

A 23-G vitrectomy is safe and efficient for the surgical management of dropped lens fragments following cataract surgery.     

糖尿病新生血管性青光眼23G玻璃体视网膜手术

目的 探讨23G玻璃体视网膜手术治疗糖尿病性新生血管性青光眼的效果。方法 回顾性研究22例（22眼）糖尿病性新生血管性青光眼先作青光眼引流阀植入控制眼压,在1月内作23G玻璃体视网膜手术。术后随访6—12月,观察眼压变化。结果 16例手术后眼压在正常范围,4例需要加用降压药物控制,2例眼压未能控制。视力光感3例,手动2例,数指4例,0．05—0．1者7例,0．12～0．3者4例,0．4以上2例。术后复发性视网膜脱离3例;2例再次出现虹膜新生血管;前房积血7例,1—2周后吸收;取出硅油后再次玻璃体积血2例,行玻璃体腔冲洗及补充激光光凝;视神经萎缩2例;前部葡萄膜炎2例。结论 青光眼引流阀植入和23G玻璃体视网膜手术治疗糖尿病性新生血管性青光眼是一个较有效的治疗方法。     

23G微创玻璃体切割术治疗Terson综合征的疗效观察

目的：探讨23G微创玻璃体切割术治疗Terson综合征的疗效。

方法：对伴有严重玻璃体积血的6例11眼Terson综合征患者在保守治疗无效后行23G微创玻璃体切割术,术中根据视网膜情况行剥膜或硅油填充,观察术后视力、视网膜及并发症情况。

结果：术后所有患者的视力均有明显提高,11眼中8眼(73%)术后视力≥0.5,1眼(9%)因术前存在严重的玻璃体视网膜增殖性病变和视网膜脱离,术后视力低于0.1。

结论：23G微创玻璃体切割术是治疗Terson综合征的有效手段,大部分患者术后视力恢复较好,但增殖性病变严重的患者术后视力欠佳,对于Terson综合征在保守治疗无效的情况下建议早期行23G微创玻璃体切割术干预。     

标准三通道下23G免缝合切口联合18G套管针硅油取出术的临床应用研究

目的探讨标准三通道下23G免缝合切口联合18G套管针硅油取出术的临床疗效。方法对84例（87只眼）有晶状体眼行单纯硅油取出术,其中标准三通道下23G免缝合切口联合18G套针硅油取出术（简称23G组）43例（45只眼）,传统20G缝合切口硅油取出术（简称20G组）41例（42只眼）,记录两组手术时间,术后随访5-10个月,平均（8.5±2.5）个月,观察两组术后视力恢复、眼压变化和并发症情况,对相关记录进行统计分析比较。结果 23G组平均手术时间（16.65±0.66）min,显著性低于20G组（21.77±0.84）min,有统计学意义（P〈0.01）。术后两组最佳矫正视力相比较,无显著性差异（P〉0.05）。术后1 d 23G组眼压显著性低于20G组（P〈0.05）,术后1周、1个月23G组眼压与20G组比较无显著性差异（P〉0.05）。术后两组并发症发生情况无显著性差异（P〉0.05）。结论标准三通道下23G免缝合切口联合18G套管针硅油取出术时间短,能降低视网膜脱离等并发症的发生几率,该手术安全有效。     

苦瓜茎叶醋酸乙酯部位的化学成分研究

目的研究苦瓜茎叶醋酸乙酯部位的化学成分,为进一步开发利用苦瓜茎叶提供依据。方法采用反复硅胶柱色谱、反相ODS柱色谱、SephadexLH-20等柱色谱进行分离纯化,并根据波谱数据鉴定化合物的结构。结果分离得到3种苦瓜三萜,分别鉴定为苦瓜素I（1）、kuguacinN（2）、3,7．二羟基．23帜）-乙氧基-19．醛基．5,24-二烯醇（3）。结论化合物3为新化合物,命名为23．乙基苦瓜素I。     

Soluble serum Klotho levels in healthy subjects. Comparison of two different immunoassays

Objective

Soluble serum Klotho is a new biomarker linked to chronic kidney disease, cardiovascular disease and diabetes. This study describes the evaluation and comparison of two different immunoassays and establishment of assay specific reference intervals in adults.

Design and methods

Serum Klotho concentrations were determined in 120 healthy adults aged 19–66 years. Blood samples were collected, and stored sera were assayed for Klotho according to age and gender. In addition several other clinical and laboratory characteristics were determined in the cohort and compared to the levels of serum Klotho.

Results

Serum Klotho levels were significantly higher in time-resolved fluorescence immunoassay (TRF) compared to an ELISA (IBL) and no correlation was found between the assays. No signal was obtained in either assay when the standard curve was switched between the two different immunoassays. The median serum Klotho concentration using TRF was 61 ng/mL (2.5–97.5% reference limits; 11–181 ng/mL) for males and 99 ng/mL (2.5–97.5% reference limits; 19–316 ng/mL) for females while the ELISA gave a mean value of 472 pg/mL (2.5–97.5% reference limits; 204–741 pg/mL) with no difference between genders. Concentrations of serum Klotho were independently associated with estimated glomerular filtration rate (eGFR) and body weight using TRF whereas serum Klotho concentrations were associated with age using the ELISA.

Conclusion

Comparison of two different immunoassays for serum Klotho indicate, that the protein exists in human beings in different forms which may function as independent factors and whose role and potential value as biomarkers needs to be evaluated separately. Reference intervals specific for the different forms recognized by the different assays were calculated in this study.