青年期大肠癌nm23/NDPK蛋白的表达及意义(附30例报告)

采用免疫组织化学S -P方法检测 30例青年期大肠癌癌组织中nm2 3 -H1基因产物 /核苷二磷酸激酶 (nm 2 3/NDPK )的表达。结果青年期大肠癌nm2 3/NDPK表达阳性率为 6 3 .33 % ,在组织分化低、原发灶浸润程度 (T3、T4 )、淋巴结及远处转移 ,DukesC、D期和血清CEA阳性的大肠癌中表达明显减低 (P <0 .0 5 ) ,与其他生物学指标无关 (P >0 .0 5 )。提示青年期大肠癌组织学类型 ,浸润转移程度与nm 2 3/NDPK蛋白的表达有密切相关 ,其有助于对青年期大肠癌生物学特性的进一步了解及对病人预后的判断     

nm23蛋白在纤支镜活检中的表达意义

应用免疫组化ＳＰ法,通过纤支镜取材或刷片研究肺癌及肺良性病的ｎｍ２３蛋白表达情况,以探索ｎｍ２３在肺癌中与肺门或纵隔淋巴结转移的关系。结果：肺癌组ｎｍ２３蛋白阳性率低于肺良性病组（Ｐ〈０．０５）,肺癌组肺门或纵隔淋巴结转移的ｎｍ２３蛋白阳性率低于无淋巴结转移组（Ｐ〈０．０５）。表明：ｎｍ２３蛋白的表达可为术前肺癌有无肺门或纵隔淋巴结转移的参考指标。     

胃癌p53,c—erbB—2和nm23癌基因蛋白的免疫组织化学研究

目的利用胃癌生物学恶性信息,探讨术前检测ｐ５３、ｃ－ｅｒｂＢ－２和ｎｍ２３蛋白对胃癌的诊断价值。方法收集胃癌手术标本１４１例,胃镜活检标本９３例,应用免疫组化技术检测ｐ５３、ｃ－ｅｒｂＢ－２和ｎｍ２３蛋白在上述组织中的表达。结果ｐ５３阳性率为４５．８％～５６．３％,ｃ－ｅｒｂＢ－２为１８．３％～３０．２％,ｎｍ２３为７２．９％～８１．３％,在非胃癌组织中无表达;ｃ－ｅｒｂＢ－２的表达与肿瘤组织的大小、癌细胞的分化程度及Ｌａｕｒéｎ分型有关,且ｃ－ｅｒｂＢ－２表达阳性组５年生存率低于阴性组;ｎｍ２３的表达与肿瘤的生长方式、浸润深度和淋巴结转移有关。三种癌蛋白的阳性率在胃癌的胃镜活检标本和手术标本中无显著差别。结论对胃癌组织进行ｐ５３、ｃ－ｅｒｂＢ－２和ｎｍ２３蛋白的检测,有助于胃癌的诊断、良恶性病变的鉴别诊断、非手术临床分期的判断和评估胃癌患者的预后。     

Plasma Factor Triggering Alternative Complement Pathway Activation by Liposomes

Several plasma components, such as complement (C) components, play a role in the clearance of liposomes from the circulation. The interactions between liposomes and the C system were investigated in this study. Multilamellar vesicle (MLV) liposomes, which were damaged by activation of the complement, became susceptible depending on the density of cetylmannoside (Man) on the liposome membrane, and activation proceeded through the alternative C pathway as observed for liposomes without Man (PC-MLV) (K. Funato et al, Biochim. Biophys. Acta 1103:198–204, 1992). In addition, the capacity of Man-modified liposomes (Man-MLV) to activate the alternative C pathway was abolished by preadsorption of plasma with Man-MLV but not with PC-MLV. The results suggest that a specific plasma factor adsorbed with Man-MLV was responsible for the augmentation of the C activation and, further, that the rapid clearance of Man-MLV from the circulation is caused by both enhanced C-mediated liposome permeability and enhanced C-mediated phagocytosis of liposomes.     

Soft Drugs. XX. Design,Synthesis, and Evaluation of Ultra-Short Acting Beta-Blockers

A new type of ultra-short acting -blocker which might prove advantageous in treating acute arrhythmias was designed, synthesized and investigated. Based on the soft drug inactive metabolite approach, the inactive phenylacetic acid metabolite of both metoprolol and atenolol was reactivated by esterification with sulfur-containing aliphatic alcohols. Since the sulfur-containing moieties are labile to the ubiquitous esterases, the new compounds should be inactivated by a one step enzymatic cleavage back to the inactive phenylacetic acid derivative. Pharmacological and pharmacokinetic profiles of the new compounds were evaluated in rats and rabbits. Isoproterenol-induced tachycardia was inhibited with short-term infusion of each compound. This tachycardia blocking effect rapidly disappeared upon termination of infusion, while -blocking activity was 2–4-fold longer after comparable doses of the short-acting -blocker, esmolol. The rapid recovery from the -receptor blockade is believed due to fast hydrolysis of the soft drugs in the body. This is supported from in vitro results showing the t_l/2 of esmolol is about 10-fold longer than the new soft drugs in rat, rabbit, dog and human blood. Hydrolysis studies in phosphate buffered solutions indicated that the esters are labile to base-catalyzed hydrolysis. However, the relative t_1/2 values measured in biological media compared to phosphate buffered solution clearly support rapid enzymatic cleavage of the soft drugs. Interestingly, one of the soft -blockers, the sulfonyl ester derivative, showed a unique property of exhibiting good -receptor blocking activity without significant hypotensive action.     

CD23单克隆抗体的研制及其鉴定

目的：制备高度特异性和高纯度的抗ＣＤ２３单克隆抗体（ＣＤ２３ＭｃＡｂ）。方法：用ＥＢＶ感染的人Ｂ淋巴细胞（ＲＰＭＩ－８８６６）免疫ＢＡＬＢ／Ｃ小鼠,采用杂交瘤技术,细胞ＥＬＩＳＡ和Ｗｅｓｔｅｒｎ－ｂｌｏｔｉｎｇ等方法制备、纯化和鉴定了ＣＤ２３ＭｃＡｂ。结果：筛选出了强分泌抗Ｂ细胞膜ＣＤ２３分子的单克隆抗体的杂交瘤细胞株（２Ｂ６）。结论：该单抗对Ｍｒ４５×１０３的ＣＤ２３分子反应具有高度特异性。     

nm23-H_1基因与突变型P~(53)基因在人乳腺病变组织中的表达

目的观察７６例乳腺病变组织中ｎｍ２３－Ｈ１及突变型Ｐ５３基因的表达情况,分析其与乳腺癌生物学行为及淋巴结转移的关系。方法应用ＳＰ免疫组织化学方法检测７６例乳腺病变组织中ｎｍ２３－Ｈ１及突变型Ｐ５３蛋白表达。结果ｎｍ２３－Ｈ１基因在５６例乳腺癌中表达率为４４．６４％,１０例乳腺纤维瘤均阳性,１０例纤维囊性病９例阳性,后二者均高于乳腺癌中阳性率（Ｐ＜０．００５,Ｐ＜０．０１）;Ｐ５３在乳腺癌中阳性率３５．７１％,在所有良性乳腺疾患中均阴性（Ｐ＜０．０２５）。同时发现ｎｍ２３－Ｈ１表达与乳腺癌组织分化程度及淋巴结转移呈负相关（Ｐ＜０．０２５,Ｐ＜０．０５）,与病理组织类型无关;Ｐ５３表达与乳腺癌淋巴转移正相关（Ｐ＜０．０５）,与组织类型及分化程度无关;ｎｍ２３－Ｈ１与Ｐ５３表达呈负相关（Ｐ＜０．０５）;另外,ｎｍ２３－Ｈ１表达缺失与Ｐ５３的阳性表达在乳腺癌淋巴结转移上有协同作用。结论ｎｍ２３－Ｈ１的表达缺失及Ｐ５３的阳性表达提示乳腺肿瘤恶性度高、淋巴转移潜能大,二者联合检测对乳腺癌患者预后的评价更为全面可靠。     

nm23基因在大肠癌组织中的表达及临床意义

目的研究nm23基因在大肠癌组织中的表达及临床意义。方法用免疫组化方法对63例大肠癌患者骨髓内转移癌细胞及肿瘤组织内nm23表达进行了测定。结果骨髓内转移癌细胞阳性者原发灶nm23蛋白表达率53．1％,低于骨髓内转移癌细胞阴性者的表达率77．4％,有统计学差别（P＜0．05）。发生肝、肾及腹腔广泛转移者原发灶表达率50．0％,低于未发生转移者的表达率81．8％（P＜0．05）。nm23蛋白表达与淋巴结转移无显著相关性。结论nm23蛋白表达与远处转移及骨髓内转移癌细胞呈负相关性,可作为预测转移和判断预后的标志物。     

Influence of environmental and genetic factors on variation in human response to DNA damaging agents

Exogenous DNA damaging agents must be considered in the context of endogenous reactive species which have the potential to damage DNA. Although a no-effect level for a DNA-damaging compound may not exist, it may be feasible to define a level where reducing exposure to the compound is no longer the most effective way of reducing human risk. Modifying environmental factors which affect human response to damage may be the better strategy. Although a number of rare human syndromes are associated with a reduced ability to repair DNA damage, it is not clear how wide is the range of genetic variation in repair capacity among normal individuals. Studies with DNA repair-deficient human syndromes indicate that processes other than mutation and DNA repair must be involved in the development of cancer, and these processes may represent new sources of variation in human response to genotoxic agents.     

nm23-H1 expression in squamous cell carcinoma of the head and neck

Archival material from 47 patients with primary squamous cell carcinoma of the head and neck (SCCHN) was studied immunohistochemically for the presence of nm23-H1 protein. Our data indicate that nm23-H1 protein expression is a common event in SCCHN and that there is a trend toward correlation of increased expression of nm23-H1 with increasing tumor size (p = 0.072). The results also show that when adjusting for age and cause of death, there tended to be an inverse relationship between overall survival and the expression of nm23-H1 gene in the primary tumor (p = 0.088).