FAK在乳腺癌组织和癌前病变组织中的表达

目的探讨黏着斑激酶（FAK）的表达与乳腺癌浸润和转移的关系。方法采用免疫组织化学和原位杂交方法检测42例乳腺癌组织、32例乳腺增生组织及20例正常乳腺组织中FAK的表达，并分析其与临床病理特征之间的关系。结果1）乳腺癌组织中FAK蛋白及mRNA阳性表达率分别为83．33％（35／42），78．57％（33／42），在不典型增生中阳性表达率分别为52．38％（11／21）和42．86％（9／21），在一般增生和正常乳腺组织中均未见表达。乳腺癌组显著高于正常组、一般增生组和不典型增生组（P〈0．01），不典型增生组也显著高于正常组和一般增生组（P〈0．05）；2）与无淋巴结转移的乳腺癌比较，有淋巴结转移的乳腺癌中FAK蛋白及mRNA阳性率较高，差异具有统计学意义（P〈0．05）；3）FAK蛋白及mRNA阳性表达率与乳腺癌的组织学类型无关，随着乳腺癌的临床分期的升高，FAK蛋白及mRNA的表达有着增高的趋势，但两组间进行比较，差异无统计学意义（P〉0．05）。结论FAK表达的高低可能作为乳腺癌发生、发展过程中较有价值的病理指标，可导致乳腺癌发生并加速恶性细胞的增殖、浸润和转移。     

血管内皮生长因子在非小细胞肺癌组织中的表达及其临床意义

目的探讨血管内皮生长因子（vascular endothelial growth factor，VEGF）在NSCLC中表达及其临床病理特征之间的关系。方法应用免疫组织化学技术检测100例NSCLC和60例肺良性病变组织中VEGF的表达。结果VEGF在NSCLC中的表达率高于肺良性病变组（P〈0．01），并且与患者的年龄、性别、肿瘤的大小、分化程度以及组织类型之间比较差异无显著性（P〉0.05）。而在有淋巴结转移组和Ⅲ期＋Ⅳ期组的表达分别显著高于无淋巴结转移组和Ⅰ期＋Ⅱ期组（P〈0．05）。结论VEGF表达与NSCLC的病程分期和淋巴结转移密切相关，可作为评估NSCLC病程、转移潜力和判断预后的一个重要标志物。     

FAK、UPA在胃腺癌中的表达及其临床意义

目的　研究FAK、UPA在胃腺癌中的表达与其浸润和转移的关系。方法　应用免疫组织化学方法监测癌旁组织 ,癌组织中FAK、UPA蛋白的表达情况。结果　FAK、UPA在胃腺癌中的表达明显高于正常胃组织 (P <0 0 1)。有淋巴结转移组较无淋巴结转移组表达高 (P <0 0 1)。浸润程度越深FAK、UPA表达越高 (P <0 0 1)。FAK与UPA的表达呈正相关 (P <0 0 1)。结论　FAK、UPA在胃腺癌的浸润和转移中起到了重要作用 ,FAK、UPA在胃腺癌中表达升高提示其浸润和转移的高风险性。     

Epikeratophakia: histopathological and cultural study

Summary Three epikeratoplasty buttons (one aphakic and two myopic) prepared by the freeze technique were removed two to nineteen months after surgery. A complete morphological and immunohistochemical study was performed on these buttons in order to gain insight into the reasons for epikeratophakia failure. Histopathological studies with light microscopy and scanning and transmission electron microscopy were performed. All three cases showed an anomalous process of re-epithelialization: in the first, the epithelium over the donor cap was almost completely absent, with many abnormalities; in the second, the epithelium was irregular, with a varying number of cell layers and poor adhesion between cells; in the third, the development of an epithelial cyst between the tissue lens and the cornea of the host caused the failure of the epikeratoplasty.The specimens were cultured in vitro and the cells grown typed for HLA antigens. The antigen panel was the same as that of the host. Immunohistochemistry showed CD3- and CD8-positive cells in the stroma, proving the activity of T-suppressor lymphocytes in a cell-mediated immunoreaction.     

BMP在48例骨及软组织肿瘤的免疫组化研究

以骨形态发生蛋白(BMP)的单克隆抗体ABC法,对36例骨肿瘤、12例软组织肿瘤进行免疫组化染色。结果12例软组织肿瘤阴性(纤维瘤2例,横纹肌肉瘤3例,平滑肌肉瘤7例),骨肿瘤阳性占89%(除外1例骨的恶性纤维组织细胞瘤,1例软骨肉瘤和2例间叶性软骨肉瘤)。认为:①BMP-McAb可用于区分肿瘤是否起源于成骨性结缔组织;②鉴于许多骨肿瘤皆有BMP,但生物学检测只有骨肉瘤、软骨肉瘤和骨样骨瘤有骨生成反应,提出可能和BMP含量有关,并认为免疫组化法优于生物学检测。     

Immunohistochemical demonstration of 14-3-3 sigma protein in normal human tissues and lung cancers,and the preponderance of its strong expression in epithelial cells of squamous cell lineage

In order to confirm 14-3-3 sigma (sigma) protein distribution in human tissues, immunohistochemistry was performed using various paraffin-embedded human tissues. In normal human tissues, the strongest immunoreactivity for 14-3-3sigma protein was observed in squamous epithelia at various sites, followed by basal cells of the trachea, bronchus and basal or myoepithelial cells of various glands. Moderate to weak 14-3-3sigma immunoreactivity was seen in the epithelial cells of the alimentary tract, gall bladder, urinary tract and endometrium. In the lung, 14-3-3sigma immunoreactivity was also observed in hyperplastic type II alveolar cells and metaplastic squamous cells. Immunohistochemical study using non-small-cell lung cancers revealed that 14-3-3sigma immunoreactivity was stronger in squamous cell carcinomas than in adenocarcinomas. The present study revealed that 14-3-3sigma expression was exclusively present in various epithelial cells and had a tendency to be stronger in cells destined for squamous epithelium or differentiating toward squamous cells in human normal and neoplastic cells.     

29例胃肠道间质瘤临床病理分析

目的探讨胃肠道间质瘤的临床病理,免疫组化和组织分型.方法29例胃肠道间质瘤进行常规检查及免疫组化染色检测.结果胃肠道间质瘤好发于胃和小肠,常见的临床症状为消化道出血,腹痛.29例中Vimentin,SMA,S-100,CD34阳性分别为96.5%,41.3%,17.2%,86.2%.结论胃肠道间质瘤虽然光镜下形态相似,但具有多向分化特征,免疫组化在诊断中起重要作用,CD34有较高特异性和敏感性.     

CC趋化因子受体6在人唾液腺腺样囊性癌组织中的表达及其临床意义

目的：探讨CC趋化因子受体6（CCR6）在人唾液腺腺样囊性癌组织中的表达,阐明其在人唾液腺腺样囊性癌神经侵袭中的作用机制。方法：利用免疫组织化学染色（SP法）检测30例唾液腺腺样囊性癌患者癌组织（根据癌细胞是否沿神经束周围或神经束间浸润生长,分为神经侵袭组18例,非神经侵袭组12例）及22例正常人唾液腺组织中CCR6的表达情况,分析癌组织中CCR6阳性表达率与患者临床病理特征的关系。结果：在30例唾液腺腺样囊性癌组织中22例CCR6呈阳性表达,阳性表达率为73.33%,免疫组织化学染色显示CCR6主要表达于癌细胞的细胞膜和细胞质中;在22例正常唾液腺组织中CCR6均呈阴性表达,2组CCR6阳性表达率比较差异有统计学意义（P<0.05）。其中,神经侵袭组和非神经侵袭组唾液腺腺样囊性癌患者癌组织中CCR6阳性表达率分别为94.44%（17/18）和41.67%（5/12）,组间比较差异有统计学意义（P<0.05）;在不同年龄、性别和病理分型的唾液腺腺样囊性癌患者癌组织中CCR6的阳性表达率比较差异均无统计学意义（P>0.05）。结论：CCR6在人唾液腺腺样囊性癌的发生发展过程中起重要作用,且与其神经侵袭有密切关联。     

肝细胞癌组织MTA1和MMP-2表达及其相关性研究

目的:研究肝细胞癌(HCC)组织中转移相关基因1(MTA1)及基质金属蛋白酶-2(MMP-2)蛋白的表达及其临床意义,并探讨其与HCC生长及浸润转移的关系.方法:采用免疫组化法检测60例HCC组织及癌旁肝组织中MTA1和MMP-2的表达.结果:MTA1及MMP-2在癌组织中的阳性率分别为65.00%和60.00%,而在癌旁组织中的阳性率分别为35.00%和26.67%,癌组织与癌旁组织比较差异有统计学意义,P<0.05.MTA1在人HCC组织中的表达与临床分期、术后复发、肝外转移及门静脉癌栓相关.MMP-2在人HCC组织中的表达与临床分期、门静脉癌栓、肝外转移及术后复发相关.在癌组织中MTA1与MMP-2的表达呈正相关.结论:HCC中MTA1及MMP-2的高表达与HCC生长及浸润转移有关,在HCC的发生、发展及术后复发过程中起重要作用.     

Expression and significance of p53,nm23 and p16 in Wilms' tumor of children

With the progress in research of cancers,it was found not only oncogene but also supress oncogene was involved in the mutation.p53,p16 and nm23 were three kinds of supress oncogene and contributed to the development of tumors.The mutation,dificency and over expression of aboved genes were common in malignant tumors rather than solid tumors of children.Wu yeming had conducted a lot of studies on embryo bud tumors.The objective of the current study was to furtherly investigate the expression and…