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本文利用条形码技术建立"无纸化"检验流程,设置流程监控节点,用实验室信息系统(LIS)的回报时间(TAT)分析功能对流程进行评测。研究显示LIS可以有效地对检验流程进行监管和优化。 相似文献
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临床实验室信息系统(Laboratory Information System, LIS)是医院管理信息系统中的一个重要组成部分。以天津市肿瘤医院临床实验室信息系统的开发与应用为背景,对系统建设进行需求分析制定详细的工作流程,采用UML建立系统用例模型,采用面向对象方法完成系统架构设计,使用Caché作为数据库和开发工具,通过全条码的流程实现与医院HIS系统无缝对接,形成一套功能比较完善的临床实验室信息系统。通过LIS系统建设,最终实现样本管理条码化,设备通讯双向化,检验流程自动化,检验报告无纸化,审核收费并网化,门诊报告取单自助化,实验室管理信息化。 相似文献
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本文探讨了我院利用条形码技术,引进医疗器械追溯管理系统,实现了植人性高值耗材使用的科学性、规范性、安全性,健全了医疗器械管理的监管制度。 相似文献
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The ambitious idea of using a short piece of DNA for large-scale species identification (DNA barcoding) is already a powerful tool for scientists and the application of this standard technique seems promising in a range of fields including forensic genetics. While DNA barcoding enjoyed a remarkable success for animal identification through cytochrome c oxidase I (COI) analysis, the attempts to identify a single barcode for plants remained a vain hope for a longtime. From the beginning, the Consortium for the Barcode of Life (CBOL) showed a lack of agreement on a core plant barcode, reflecting the diversity of viewpoints. Different research groups advocated various markers with divergent set of criteria until the recent publication by the CBOL–Plant Working Group. After a four-year effort, in 2009 the International Team concluded to agree on standard markers promoting a multilocus solution (rbcL and matK), with 70–75% of discrimination to the species level. In 2009 our group firstly proposed the broad application of DNA barcoding principles as a tool for identification of trace botanical evidence through the analysis of two chloroplast loci (trnH-psbA and trnL-trnF) in plant species belonging to local flora. Difficulties and drawbacks that were encountered included a poor coverage of species in specific databases and the lack of authenticated reference sequences for the selected markers. Successful preliminary results were obtained providing an approach to progressively identify unknown plant specimens to a given taxonomic rank, usable by any non-specialist botanist or in case of a shortage of taxonomic expertise. Now we considered mandatory to update and to compare our previous findings with the new selected plastid markers (matK + rbcL), taking into account forensic requirements.Features of all the four loci (the two previously analyzed trnH-psbA + trnL-trnF and matK + rbcL) were compared singly and in multilocus solutions to assess the most suitable combination for forensic botany.Based on obtained results, we recommend the adoption of a two-locus combination with rbcL + trnH-psbA plastid markers, which currently best satisfies forensic needs for botanical species identification. 相似文献
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Hin-Fung Tsang Vivian Weiwen Xue Su-Pin Koh Ya-Ming Chiu Lawrence Po-Wah Ng 《Expert review of molecular diagnostics》2017,17(1):95-103
Introduction: Formalin-fixed, paraffin-embedded (FFPE) tissue sample is a gold mine of resources for molecular diagnosis and retrospective clinical studies. Although molecular technologies have expanded the range of mutations identified in FFPE samples, the applications of existing technologies are limited by the low nucleic acids yield and poor extraction quality. As a result, the routine clinical applications of molecular diagnosis using FFPE samples has been associated with many practical challenges. NanoString technologies utilize a novel digital color-coded barcode technology based on direct multiplexed measurement of gene expression and offer high levels of precision and sensitivity. Each color-coded barcode is attached to a single target-specific probe corresponding to a single gene which can be individually counted without amplification. Therefore, NanoString is especially useful for measuring gene expression in degraded clinical specimens.
Areas covered: This article describes the applications of NanoString technologies in molecular diagnostics and challenges associated with its applications and the future development.
Expert commentary: Although NanoString technology is still in the early stages of clinical use, it is expected that NanoString-based cancer expression panels would play more important roles in the future in classifying cancer patients and in predicting the response to therapy for better personal therapeutic care. 相似文献
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该研究通过分析多途径来源的新疆桑属植物及药材样本的ITS2,psbA-trnH序列,为药材分子鉴定提供依据。以51个新疆桑属植物及药材为样本,对其ITS2,psbA-trnH序列进行PCR扩增和测序,用MEGA 6.0计算其种内、种间Kimura 2-parameter(K2P)距离,分析变异位点,并构建NJ鉴别树。ITS2序列分析结果显示,桑Morus alba、鞑靼桑M.alba var.tatarica、黑桑M.nigra种内无变异;桑与鞑靼桑种间无变异;桑与黑桑种间存在13个变异位点,种间平均KP2遗传距离为0.04;桑与药材样本间无信息变异位点,NJ鉴别树可将桑及鞑靼桑与黑桑区分。psbA-trnH序列分析结果显示,桑与黑桑种内各有1个变异位点,3种植物种间存在插入/缺失变异,可相互区分;种间变异与药材样本内变异一致。因此,ITS2序列可将来源于桑、鞑靼桑的药材样本与黑桑区分,psbA-trnH序列可将来源于三者的药材样本区分,为维吾尔药材真伪鉴别及市场监管提供依据。 相似文献