中国地理资源期刊集群平台期刊特约专栏实施成效分析与启示

【目的】 依据中国地理资源期刊集群平台期刊特约专栏出版实践,分析实施成效,总结出版经验,为同行组织特约专栏提供借鉴。【方法】 采用中国知网的统计数据,分析中国地理资源期刊集群7种平台期刊在2016—2017年刊发的特约专栏的表现。【结果】 特约专栏整体上能够提高期刊影响力,推广学术传播,但不同类型的特约专栏的表现存在差别。【结论】 内容型专栏是提升期刊影响力的有效措施,双盲评审、相似主题的间隔时间及同一作者的出现频次是特约专栏成效的重要影响因素。特约专栏的组织需要与科技创新一致,落实“四个面向”的战略部署。     

Medical equipment libraries: implementation,experience and user satisfaction

Abstract

The hospital-wide pooling and sharing of certain types of medical equipment can lead to both significant improvements in patient safety and financial advantages when compared with a department or ward-level equipment ownership system. In September 2003, a Medical Equipment Loan Service (MELS) was established, focusing initially on infusion pumps. The aims and expected benefits included; improving availability of equipment for both patients and clinical users, managing and reducing clinical risk, reducing equipment diversity, improving equipment management and reducing the overall cost of equipment provision. A user survey was carried out in 2005 and repeated in 2011. The results showed wide and continued satisfaction with the service. The process and difficulties of establishing the service and its development to include additional types of equipment are described. The benefits of managing medical equipment which is in widespread general use, through a MELS as part of a Clinical Engineering Department, are presented.     

High-Efficiency Cloning of DNA Sequences Complementary to Mouse Neuroblastoma Polyadenylated RNA

A cDNA library was efficiently synthesized from mouse neuroblastoma poIy(A)⁺RNA. Several modifications of the oligo(dC)(dG) tailing procedure were used. After first strand synthesis, a dATP tail was added to the 3′-end of the cDNA. The second strand was primed for synthesis with oligo(dT). Blunt ends were produced on the cDNA by treatment with S1 nuclease. Size-enriched fractions of high molecular weight DNAs were obtained by passing the cDNA over a Sepharose CL-4B column. The optimal tailing time for each cDNA fraction was individually tested. Tailing reactions used terminal deoxynucleotidyl transferase and annealing reactions used a (G)-tailed PstI cut pBR322. E. coli K12 RR1 cells were transformed and 2.5–5 × 10⁶ transformants per μg cDNA insert were obtained for each size fraction. The transformants had an average insert size of 1200 base pairs and were 98% ampicillin sensitive. Our modifications in the method for cDNA library synthesis had 3 advantages. (1) Homopolymer-primed cDNA treated with S1 nuclease allowed the blund ends to be tailed sychronously. This allowed a higher transformation efficiency without loss of 5′-sequences. (2)Time tailing determined the most efficient tail length and optimized the transformation efficiency in each size fraction. (3) A Sephadex G-50 mini-column was used to desalt and dry nitrogen was used to concentrate the ds cDNA instead of the usual ethanol precipitation. This resulted in almost 100% recovery of synthesized products at each step of this procedure.     

医院图书馆信息服务面临的问题与对策

如今已进入信息时代,在信息技术飞速发展的推动下,各行各业的发展模式推陈出新,图书馆同样如此,传统的图书馆服务模式在信息服务的改变下,服务水平得到了有效提升。文章就当前我国医院图书馆信息服务中面临的问题展开研究分析,探讨针对的解决对策。     

真实世界肺癌免疫治疗的有关问题和思考

近年来,免疫治疗研究在肺癌上取得突破性进展,能显著改善肺癌患者的总生存期,且耐受性良好。然而临床研究入组人群具有高度选择性,并不能完全代表真实世界人群。真实世界免疫治疗的疗效及安全性是否与临床研究的一致,何时应用,选择单药还是联合治疗,老年、PS≥2分、自身免疫疾病、使用激素、脑转移等特殊人群的应用,以及疗效标志物的选择,疗效评估标准,不良反应的管理等问题都值得进一步思考。本文通过真实世界数据与临床研究的比较,对真实世界免疫治疗存在问题进行初步分析。     

Qui sont les élèves à besoins éducatifs particuliers ? La part d’aléatoire du signalement pour une mesure d’aide renforcée de pédagogie spécialisée dans un canton suisse

In this article, we begin by questioning the notion of special educational needs, which is used today in Switzerland, by going back over its historical foundations and developing the main issues and problems it raises. We then present the results of a comprehensive research aimed at uncovering the factors at work in the process of designating certain students for an enhanced special education support measure during their compulsory schooling. On the basis of a content analysis of the reports for an enhanced special education support measure produced during the 2016–2017 school year in a Swiss canton, as well as comprehensive interviews conducted with permanent teachers, we highlight various factors that can lead to the designation of a student for an enhanced special education support measure. We show how there is a degree of randomness in being designated for a special education support measure and critically discuss the role of the school institution and its actors in this process.     

Exploring the longitudinal association between interventions to support the transition to secondary school and child anxiety

School transition at around 11-years of age can be anxiety-provoking for children, particularly those with special educational needs (SEN). The present study adopted a longitudinal design to consider how existing transition strategies, categorized into cognitive, behavioral or systemic approaches, were associated with post-transition anxiety amongst 532 typically developing children and 89 children with SEN. Multiple regression analysis indicated that amongst typically developing pupils, systemic interventions were associated with lower school anxiety but not generalized anxiety, when controlling for prior anxiety. Results for children with SEN differed significantly, as illustrated by a Group × Intervention type interaction. Specifically, systemic strategies were associated with lower school anxiety amongst typically developing children and higher school anxiety amongst children with SEN. These findings highlight strategies that schools may find useful in supporting typically developing children over the transition period, whilst suggesting that children with SEN might need a more personalized approach.     

宫颈癌HeLa细胞特异性结合肽的筛选及鉴定

背景与目的:宫颈癌的分子靶向治疗具有很好的疗效,同时可以显著减少抗癌药物对人体自身的损伤,因此备受关注。本研究利用噬菌体体内展示技术筛选及鉴定宫颈癌特异性结合肽,将有可能成为化疗药物的靶向载体,为宫颈癌靶向药物治疗奠定基础。方法:体外培养宫颈癌HeLa细胞接种裸鼠,建立肿瘤动物模型。将随机肽库尾静脉注入裸鼠体内,循环15 min,心脏灌注后回收肿瘤组织噬菌体扩增、纯化并以此作为起始物进行第2轮的筛选,如此进行3轮体内筛选后挑取噬菌体克隆,进行免疫组化及ELISA实验,初步鉴定噬菌体克隆对宫颈癌细胞的亲和力及特异性,并将具有强亲和力的克隆进行测序。结果:ELISA结果显示,随机挑选10个噬菌体单克隆中8个克隆对HeLa细胞具有很强的亲和力,将这8个克隆进行测序,获得相同短肽序列LLRSTGF。结论:利用噬菌体展示技术筛选出与宫颈癌细胞HeLa特异性结合的短肽,进一步与化疗药物结合,为宫颈癌靶向治疗提供新的方法。