Feasibility study on the use of polyallyldiglycol-carbonate cell dishes in TUNEL assay for alpha particle radiobiological experiments

In the present work, we have studied the feasibility of a method based on polyallyldiglycol-carbonate (PADC) films to investigate the effects of alpha particles on HeLa cervix cancer cells. Thin PADC films with thickness of about 20 μm were prepared from commercially available CR-39 films by chemical etching to fabricate custom-made petri dishes for cell culture, which could accurately record alpha particle hit positions. A special method involving “base tracks” for aligning the images of cell nuclei and alpha particle hits has been proposed, so that alpha particle transversals of cell nuclei can be visually counted. Radiobiological experiments were carried out to induce DNA damages, with the TdT-mediated dUTP Nick-End Labeling (TUNEL) fluorescence method employed to detect DNA strand breaks. The staining results were investigated by flow cytometer. The preliminary results showed that more strand breaks occurred in cells hit by alpha particles with lower energies. Moreover, large TUNEL positive signals were obtained even with small percentages of cells irradiated and TUNEL signals were also obtained from non-targeted cells. These provided evidence for the bystander effect.     

计算机软件在多媒体化学教育中的应用

本文阐述了计算机软件在多媒体化学课件开发中的应用，着重介绍了一些普通软件及专业化学软件的特点及功能。     

新安江模型参数优选的改进粒子群算法

借鉴竞争演化和多种群混合的思想,对粒子群算法(PSO)进行改进,建立并行种群混合进化的粒子群算法(PMSE-PSO)和序列主-从种群混合进行的粒子群算法(SMSE-PSO)。数值模拟结果表明,这两种改进的粒子群算法具有较高的计算效率、较强的自适应性和稳定性。将PMSE-PSO和SMSE-PSO应用于新安江模型的参数优选中,通过与PSO和SCE-UA的比较可以看出,PMSE-PSO和SMSE-PSO不仅具有较好的全局优化性能和稳定性,而且在调用目标函数次数相同的情况下精度较高,是一种有效的新安江模型参数优选方法。     

Solid State Concepts to Understand Catalysis Using Nanoscale Metallic Particles

New advances in theoretical background and recent experimental results concerning nanometer scale metallic clusters are presented. We will begin by a presentation of the advantages and the limitations of synchrotron radiation techniques to characterise nanometer scale entities. Then, through a review of recent papers, we try to make a link between metallic surfaces and nanometer scale monometallic clusters in the case of the interaction with a small molecule, namely NO.     

“三软”煤层综放面特殊情况下支架拆除及沿空留巷

超化煤矿21091(西)综采工作面因出水造成工作面停产,在不超前15 m铺金属网的情况下进行支架拆除,并把原工作面留做炮采面切巷。根据实际情况制定了施工方案,并介绍了实施情况。     

垫层载荷试验应注意的几个问题

介绍了垫层上载荷试验的类型，通过工程实例对不同类型垫层上的载荷试验应注意的问题进行了分析和探讨，这对通过载荷试验确定双层地基承载力具有一定的实际意义，可供同类工程参考借鉴。     

超微粒子的量子阻尼振动理论

根据SU(1,1)Lie代数,建立超微粒子的量子阻尼振动理论,得到超微粒子量子阻尼振动系统的能级和波函数。     

湖泊藻类叶绿素-α和悬浮物浓度的高光谱定量遥感模型研究

本文利用地物光谱仪对太湖水体进行了光谱测量和同步采样分析，对获得的数据尝试用光谱分离法进行分析，从中分离出蓝藻和悬浮物的特征波峰，建立波峰高度与同步水质采样得到的叶绿素-α浓度和悬浮物浓度的对应关系，得出其遥感定量反演算法，并实际应用在OMIS成像光谱仪图像上．     

用激光粒度分析仪测定二氧化锆粒度

介绍以六偏磷酸钠作为分散剂，采用激光粒度分析仪测量二氧化锆粒度的一种新方法。该方法的测量范围为0．05～300μm。     

Heat transfer mechanism of a swirling impinging jet in a stagnation region

Heat transfer characteristics of a swirling impinging jet have been experimentally examined using a combined particle image velocimetry (PIV) and laser‐induced fluorescence (LIF) technique for simultaneous measurement of velocity and temperature fields. The present study shows that the radial width of the jet stretches with increasing swirl intensity, and that the stretching phenomenon contributes to the maximum local heat transfer coefficient. At the stagnation region, the flow near the heated surface is mixed intermittently by reverse flows toward upstream, and spatial distributions of temperature are correlated with instantaneous velocity vector maps. The dynamic behavior of recirculation zones, attributed to swirl number Sw and impinging distance, mainly determines the turbulent heat transfer at the stagnation region. © 2003 Wiley Periodicals, Inc. Heat Trans Asian Res, 32(8): 663–673, 2003; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/htj.10120