枸杞叶保健茶的研制

依据枸杞叶所含的营养成分和保健功能因子介绍了枸杞叶的保健作用.以枸杞叶为原料,开发研制具有保健作用的枸杞叶保健茶.探索了枸杞叶保健茶的加工工艺.     

不结球白菜(Brassica campestris ssp. chinensis Makino)种质资源SRAP遗传分化分析

利用SRAP分子标记分析了国内外64份不结球白菜种质资源的DNA遗传多样性和遗传分化。21对引物组合共检测出215个位点，其中112个为多态性位点，多态性比率达52.09%，平均每对引物组合产生10.24个位点和5.33个多态性位点。不结球白菜各类型中普通白菜的Nei’s基因多样性指数（0.1410）和遗传丰富度[(190) 88.37%]最高；各生态区域中江淮流域不结球白菜的Nei’s基因多样性指数（0.1451）和遗传丰富度[(185) 86.05%]最高；国内的Nei’s基因多样性指数(0.1293)和遗传丰富度[(188) 87.44%]分别高于国外。遗传变异估算表明，不结球白菜遗传分化系数58.22%，大部分变异存在于种群间；基因流为0.4031，说明群体间基因流动较少，遗传分化程度较高。以遗传相似系数0.872为截值，可把不结球球白菜分为Ⅵ个类群。     

Callus Induction of Aloe vera L var chinensis (Haw) Berg

Studies were given to the induction and growth of callus of Aloe vera L.var.chinensis(Haw.) Berg when the young stem was applied as explant.MS was taken as basic medium,added with various concentrations of auxins(IAA,IBA,NAA and 2,4-D),cytokinins(6-BA,KT and Zeatin) and the different combinations of these plant growth regulators.Results showed that the callus is barely induced by cytokinin alone,and the callus induction rates have significant difference when different concentrations of auxin and different combinations of auxin with cytokinin are used on the MS medium.In order to inhibit callus browning during culturing,various concentrations of vitamin C or active carbon are added into four combinations of auxin and cytokinin respectively,which can induce callus most effectively.The result showsd that vitamin C can alleviate callus browning more effectively than the active carbon.The most efficient medium for induction and growth of callus of Aloe vera L.var.chinensis(Haw.) Berg was MS+2.0 mg/L 6-BA+0.5 mg/L 2,4-D+10 mg/ L vitamin C,with which the callus induction rate can reach to 91.4% and callus grow vigorously.     

糯米糍荔枝叶片矿质养分含量充足范围的确定

对深圳市4个典型丰产园糯米糍荔枝叶片7种矿质养分(氮、磷、钾、钙、镁、锌和硼)含量的统计分析表明:采样地点和生育时期均对叶片营养元素含量有显著的影响。四个关键生育时期(末次秋梢老熟期、花芽形态分化期、谢花期和果实成熟期)的单株样与混合(多株)样的供试矿质养分含量之间大部分没有显著差异。本研究根据丰产园糯米糍荔枝叶片混合样的分析结果提出了7种矿质养分的充足含量范围。     

红豆杉扦插育苗试验研究

对红豆杉扦插育苗进行了多年试验研究,试验结果：采用黄壤土50％＋细黄沙50％为扦插基质,插穗浸泡使用浓度为100mg／L的ABT1号生根促进剂,秋季进行扦插,扦插成活率可达80％以上。扦插环境的温度、湿度和基质的保水透气性是影响扦插成活率的重要因素。     

猕猴桃对硒的吸收、分布、积累特性的研究

大田条件下,给中华猕猴桃施以浓度分别为0．15、0．3、0．5mg／培的Na：SeO,溶液,在盛花期每30d测定果实和叶对硒的积累情况;果实成熟期测定猕猴桃各部位含硒量以及鲜重、可溶性糖含量、可溶固形物和Vc含量;结果显示：施硒后,猕猴桃各部分含硒量显著升高,并且硒在猕猴桃中累计顺序为根〉叶〉花〉果实。果实鲜重、可溶性糖含量、可溶固形物和％含量随着硒浓度的增大而升高,然而在高浓度的硒（≥0．5rag／kg）水平下,受到不同程度的抑制。施硒显著提高了猕猴桃对硒的吸收、分布、积累和改善了果实的品质。     

Characteristics of nucleotides and amino acids in cDNA sequence of xyloglucan endotransglycosylase cloned from <Emphasis Type="Italic">Anthocephalus chinensis</Emphasis>

Xyloglucan endotransglycosylase is an identified relaxation factor with functions of easing and extending of plant cell walls.Its activities are directly related to plant growth and elongation of organisms.Anthocephalus chinensis is a tall and fast growing evergreen tree species.We cloned the full cDNA sequence of AcEXT genes which is abundantly expressed in the cambium of A.chinensis.The sequence analysis of nucleotides and amino acids revealed the presence of a 1396 bp full cDNA sequence,including a 960 bp complete open reading frame(ORF) encoding a 320 amino acid protein.The deduced amino acid sequence of AcXET was homologous to the other known XET proteins and contained the conserved EIDFE catalytic site which was specific to all the XETs.Our data should serve as a foundation for further insight into AcXET gene molecular mechanisms during wood formation and cell wall engineering of woody plants.     

南方红豆杉扦插繁殖技术研究--扦插苗与实生苗光合生理特性比较

对南方红豆杉扦插苗和实生苗光合生理特性比较研究结果表明,南方红豆杉扦插苗叶重、叶绿素a和b、叶绿素总量、CO2补偿点及净光合速率、硝酸还原酶活性均小于相应树龄实生苗。     

委陵菜提取液的抑菌作用

研究了委陵菜提取液对4种细菌的抑制作用.结果表明:委陵菜提取液能有效地抑制细菌的生长,对供试菌的最低抑菌浓度金黄色葡萄球菌为6%,大肠杆菌为10%,枯草芽孢杆菌为8%,变形杆菌为12%.委陵菜提取液经121℃湿热条件处理20min仍具有一定的抑菌效应,说明委陵菜提取液具有较强的热稳定性.而且委陵菜提取液对葡萄有一定的保鲜作用.     

扎龙自然保护区羊草种群分蘖株的数量性状

[目的]为了研究扎龙自然保护区羊草种群分蘖株的数量性状。[方法]以扎龙羊草种群为研究对象,以年龄结构为切入点,对不同土壤生境条件下羊草分蘖株的数量性状进行了研究。[结果]在5~9月的取样中,扎龙保护区4种生境的羊草种群分蘖株平均数量表现为沙土>林间风沙土>草甸土>中度碱土,年龄结构最多由3个龄级组成,其中均以1龄和2龄级分蘖株占绝对优势,3龄级所占比例甚小,呈增长型年龄结构;不同生境分蘖株的生物量也表现为沙土>林间风沙土>草甸土>中度碱土,年龄组成均以1龄和2龄级分蘖株对种群物质生产的贡献大,3龄级所占比重很小,也呈增长型年龄结构。[结论]该研究为湿地的管理、开发利用提供了理论基础和理论指导。