SOD—GC脂质体的制备及包封率的测定

半乳糖神经酰胺（Galactosyl ceramide,简称GC）是一种制备脂质体的新材料,性质稳定,具独特的护肤作用,以GC为主要膜材,用乙醇注入法制备包含有超氧化物歧化酶的脂质体（SOD-GC脂质体）;用SOD蛋白含量法和SOD活性法测定包封率,经正交法优化选择,最佳制备条件为膜脂量1.0mg/mL、SOD量0.1mg/mL,缓冲液pH5.8,此时包土率可达35.68%,两种包封率测定方法结果相近,可以相互参照,这种SOD-GC脂质体有望在护肝肤品和皮肤外用药物治疗等方面应用。     

口服沙棘油对游泳小鼠肝、心、脑自由基代谢影响的实验研究

本实验以雄性ICR（Swiss Hauschka）小鼠为实验对象,将其随机分为安静对照组（A）、安静沙棘组（B）、训练即刻组（C）、训练即刻沙棘组（D）、训练恢复24小时组（E）、训练恢复24小时沙棘组（F）。经过6周游泳耐力训练后进行一次力竭性游泳,观察了各组小鼠肝脏、心肌及脑组织超氧化物歧化酶（SOD）、丙二醛（MDA）等指标。结果表明：以上各组除E与F间无显著差异外（P＞0.05）,肝脏SOD其余各 间均有显著差异（P＜0.05）,与A、C、E组相比,B、D、F组SOD活性分别升高7.39%、13.1%及-0.716%;心肌SOD除A与B与F间无显著差异外（P＞0.05）,其余各组间均有显著差异（P＜0.05）,与A、C、E组相比,B、D、F组SOD活性分别升高-0.612%、4.76%及26.0%;脑SOD除A与B,E与F,、与C间无显著差异外（P＞0.05）,其余各组间均有显著差异（P＜0.05）,与A、C、E组相比,B、D、F组SOD活性分别升高-1.95%、3.88%及0.992%。对MDA而言,肝脏除C与D、E与F间无显著差异外（P＞0.05）,其余各组均有显著差异（P＜0.05）;与A、C、E组相比,B、D、F组MDA含量分别降低25.6%、6.06%及3.73%;心肌变化与肝脏相似,与A、C、E组相比,B、D、F组MDA含量分别降低34.1%、5.05%及-2.85%;脑中各组间变化不大,与A、C、E组相比,B、D、F组MDA含量分别降低5.11%、-2.29%及0.631%。本实验的研究结果表明,沙棘油口服液能显著提高小鼠游泳运动能力,提高肝、心及脑组织抗自由基氧化的功能。     

Lipid peroxidation and antioxidant status in workers exposed to PCDD/Fs of metal recovery plants

Secondary copper smelters, which primarily utilize the waste materials that contain organic impurities, and the zinc recovery plant, which handles mostly fly ash and slag from the iron and steel industry, are major emission sources of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans (PCDD/Fs) in Taiwan. In this study, we compared the levels of erythrocyte glutathione (GSH), erythrocyte superoxide dismutase (SOD) and plasma malondialdehyde (MDA) in workers at a secondary copper smelting plant and a zinc recovery plant who may have been exposed to PCDD/Fs. Though the PCDD/F levels were higher in workers of zinc recovery plant than those of secondary copper smelting plant, no significant difference was found for serum PCDD/F levels between the two kinds of plants. We observed a significant difference in plasma MDA levels between workers at the zinc recovery plant (2.54 microM) and those at the copper smelting plant (1.79 microM). There was and a significant positive correlation between plasma MDA levels and the PCDD/Fs levels. In addition, we observed that the MDA levels were not affected by smoking and exercise status. Therefore, the data suggest that the MDA levels of the metal recovery workers are influenced by their PCDD/F exposure. The erythrocyte SOD activity in workers from the zinc recovery plant was marginally higher than that from the secondary copper plant (196 vs. 146 units/ml, p<0.06). In both plants, large variations in the MDA and SOD levels were found, especially in the high-PCDD/Fs-exposure group, which may be attributed, at least partially, to the differences in smoking status and the number of cigarettes smoked. Overall, our results indicate a higher oxidative stress in workers of the zinc recovery plant than in workers of the secondary copper smelting plant in Taiwan.     

白藜芦醇对急性脊髓损伤早期脂质过氧化反应和活性氧水平的抑制作用

目的: 探讨脊髓损伤后使用白藜芦醇(resveratrol,Res) 对脊髓损伤(SCI) 早期脂质过氧化反应和活性氧水平的抑制作用。方法: 采用重物下落撞击法制备成年大鼠的SCI 模型, 于损伤后即刻腹腔注射给予Res 50, 100 mg·kg^-1 和甲基强的松龙(MPSS)100mg·kg^-1, 测定SCI 后1, 24, 48 h 时Res 组受损脊髓组织超氧化物歧化酶(SOD) 和脂质过氧化反应产物丙二醛(MDA) 及活性氧(ROS) 水平, 并与MPSS 组进行疗效对比。结果: Res 50mg·kg^-1与100 mg·kg^-1均能够显著提高SCI 后损伤部位SOD 水平和抑制MDA 产生(P<0.01), 以48 h 为最明显;显著降低ROS 水平(P<0.01), 也以48 h 最大, 抑制率大于40 %;且有明显剂量依赖性, 作用与MPSS 相当或更优。结论: Res 可以有效抑制脊髓损伤后早期受损局部脂质过氧化反应和活性氧水平, 对脊髓损伤有潜在的保护与治疗作用。     

唐古特大黄多糖对大鼠应激性胃溃疡的保护作用

目的: 探讨唐古特大黄多糖(Rheum tanguticum polysaccharides, RTP) 对大鼠应激性胃溃疡的保护作用。方法: 用水浸束缚应激法(water immersion and restraint stress, WRS) 复制大鼠应激性胃溃疡模型, 提前灌胃给予大黄多糖, 应激6 h 后处死动物,观察溃疡指数和胃粘膜损伤程度的变化, 检测血清及胃粘膜组织中超氧物歧化酶(SOD) 和丙二醛(MDA) 的变化。结果: RTP 能明显降低应激大鼠胃粘膜溃疡指数和胃粘膜MDA 水平, 升高血清和胃粘膜SOD 活性。结论: RTP 对水浸束缚应激引起的大鼠应激性胃溃疡有明显的保护作用, 可能是大黄治疗应激性胃溃疡的有效成分之一。     

氧透射载体治疗在抗肿瘤化疗中的联合应用(附40例报告)

对４０例恶笥肿瘤病人采用抗肿瘤药物联合化疗的同时配以氧透射载体治疗,防止血像下降总有效率达８９％;血浆中超氧化物歧化酶（ＳＯＤ）的活性治疗后显著升高,对照组及治疗前比较有统计学意义（Ｐ〈０．０１）,统计有明治疗前后患者的临床症状及血像的改变差异显著,此方法操作简便、无副作用,为肿瘤病人联合化疗和延长生存期创造了条件。     

芒果叶片SOD、CAT活性与生长势的关系

为探讨芒果矮化的酶学机制,笔者进行芒果叶片超氧化物岐化酶(SOD)、过氧化氢酶(CAT)的活性与生长势的研究。结果表明芒果叶片超氧化物岐化酶活性与树体矮化成正相关,活性越高树体越矮化、活性越低树体越高大,认为超氧化物岐化酶可以作为芒果矮化预选的一项指标;芒果叶片过氧化氢酶活性在不同生长力级的种质上差异性不显著,过氧化氢酶活性与生长势存在直线线性相关关系,认为过氧化氢酶不能作为芒果矮化预选的一项指标。     

Purification and characterization of superoxide dismutase from garlic

An efficient and easily scaled up method to isolate superoxide dismutase from garlic is proposed. The separation and purification procedure consists of phosphate buffer extraction, heat treatment and a two-stage ultrafiltration process. The enzyme was purified 139-fold with a specific activity of 2867 U/mg protein and a yield of 91%. The native molecular mass of superoxide dismutase estimated by fast protein liquid chromatography on a Superose 6 column was 28 kDa. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed a single band near 14 kDa, suggesting that native enzyme was homo-dimeric. The optimal pH for enzyme activity was found to be 7.0, and at this pH the enzyme exhibited maximum activity at 50 °C in 50 mM sodium phosphate buffer. Among various metal ions examined, Cu²⁺ and Zn²⁺ exerted a positive effect on superoxide dismutase activity, whereas Hg²⁺ was found to be a strong inhibitor. The final purified enzyme had an isoelectric point of 5.1-5.4 and a sheet content of 46%, consistent with the literature values. This shows that the purified SOD folded with a reasonable secondary structure.     

芦笋SOD口服液工艺的研究

利用芦笋罐头加工中的下脚料为主要原料，枣、杭白菊、甘草、枸杞子为辅料，抽提精制后添加超氧化物歧化酶（ＳＯＤ）配兑而成一种高级营养保健品。为了避免在杀菌工艺中ＳＯＤ酶活力的损失，该工艺采用了超高温瞬时灭菌技术。     

红酵母提取SOD的研究

研究了从红酵母中提取、纯化超氧化物歧化酶(SOD)的方法和条件。所得SOD酶比活2297．20U/mg，对粗酶液的收率为89．94％，纯化倍数为11倍。