降低Pin1对大肠癌SW620细胞的增殖和凋亡能力的影响

Objective:The aim of our study was to investigate the effects of Pin1 reduction on SW620 cell proliferation and apoptosis in human colorectal carcinoma.Methods:We constructed a plasmid of RNA interfering(shRNA)for Pin1 gene(pGenesl-1-Pin1),then the plasmid was transfected into colorectal carcinoma SW620 cells line by liposome mediation.The protein expression of Pin1 was tested by Western blotting.The proliferation rate was analyzed by MTT and the apoptotic rate of cells was tested by flow cytometry.In order to explain further the effect of Pin1 in SW620 cells,the protein level of Bc1-2 was analyzed by Western blotting.Results:pGenesil-1-Pin1 plasmid was successfully constructed and confirmed by sequencing.The protein relative levels of Pin1 were 0.06±0.04 for the P-shRNA/SW620 cells,and 0.32±0.09 for the P-Con/SW620 cells.The cell growth rate of SW620 cells was slower while the apoptotic rate was increased after transfection with pGenesil-Pin1plasmid,and the apoptotic rate was 12.38%±1.55%for the P-shRNA/SW620 group.At the same time,we found that the protein expression of Bcl-2 was also reduced.The results were 0.13±0.04 for the P-shRNA/SW620 cells,and 0.36±0.08for the P-Con/SW620 cells.Conclusion:Inhibited Pin1 expression may suppress the cell proliferation and promote apoptosis of colorectal carcinoma cells in vitro.     

牛腿销轴法托架在连续梁施工中的应用

结合京福铁路客专七里街特大桥三联小跨度连续梁0^#段及直线段托架的设计施工实例,针对高墩连续箱梁0#块及直线段,着重介绍了“牛腿销轴法精轧螺纹钢对拉托架”的设计和施工技术要点,为类似工程提供借鉴经验。     

新型细纱牵伸器材及其使用效果(下)

阐述国内新研制开发的细纱牵伸器材的技术性能及其使用效果.介绍了细纱罗拉的质量要求和维护要求,指出应用新型优质的无害机械波罗拉有利于降低成纱条干不匀;介绍了SL6819-SG型细纱双滑道上罗拉轴承的结构和性能特点,双滑道上罗拉轴承前后排钢球采用相同结构,受力均匀,运转稳定;介绍了丁腈胶辊、丁腈胶圈新产品,环保型胶辊涂料、高效能胶辊胶圈专用清洁剂的技术特点和使用效果,胶辊制作与纺纱工艺配置要求;介绍了聚氨酯胶辊性能特点和初步使用效果;介绍了尼龙弹性钳口上销、T形下销、下胶圈轴承式节能张力辊、V形牵伸后牵伸区压力棒装置的纺纱效果.实践表明,优选新型细纱牵伸器材,合理配置纺纱工艺,有利于成纱质量的稳定提高.     

中西文终端辅口与常用外设的连接

中西文终端辅口（COM2）与外部设备连接，运用面广泛，涉及问题也较多。特别是不同型号之间的设备连接，经常会遇到不能正常使用的现象，要解决好这个问题，可以归结为两个方面；首先，要分析和完善终端、外部设备的端口信号的基本定义，两个端口之间的对应引脚信号的硬件连接，其次，要熟悉和掌握所使用的中西文终端对辅口操作的控制命令序列，并将此灵活的运用在设计的程序中。最终实现对终端辅口和外部设备的控制与操作。     

油泵油嘴针阀体楔横轧工艺试验研究

介绍了油泵油嘴针阀体的楔横轧工艺试验，以及在试验中遇到的问题及解决方法.     

新功能材料在活塞异形销孔制造中的应用

介绍了应用新型功能材料控制刀具变形的设计思想，分析了基于超磁致伸缩材料控制刀位变化的实际试验结果，给出了系统的软硬件设计模块。本系统已成功应用于一实际制造系统中。     

关于热管销钉螺杆的设想

为使物料在螺杆挤出机或注射成型机中温度均一，螺杆上除开有中心孔外，还有四周分布的径向热管销，此销穿过螺杆，一端伸出在螺杆外，一端伸入至中心孔内。销可以是全部密封的内部充填一定数量工作液的热管，也可以伸入到中心孔内端是敞口的，螺杆本身也可做成＂热管＂。新型销钉螺杆具有极高的传热能力和传热速度，有利于低温挤出。     

拔销器作用过程的仿真研究

针对活塞式拔销器,建立了其内弹道模型,利用MATLAB软件数值模拟了装置整体作用过程,并与试验结果进行对比。结果表明内弹道模型仿真结果和试验结果基本一致,均可反映活塞式拔销器的运动特性。采用仿真模型分析各参数变化对拔销器性能的影响。结果表明活塞质量对拔销器运动的峰值速度影响最大,装药量、火药力和初始容积对峰值压力影响较大。     

考虑角点不连续因子的精细功率重构及验证

在采用调制法进行组件精细功率重构时,由于改进的格林函数节块法程序引入了组件不连续因子,导致重构时角点中子通量不连续,需引入角点不连续因子进行修正保证其连续性。文中利用改进格林函数节块法程序堆芯扩散计算的结果,采用高阶多项式展开的调制法来进行组件内的精细功率重构,探讨了角点不连续因子在精细功率重构中的重要作用。并通过秦山二期实际堆芯的两种工况对其进行了验证,与SIMULATE-3的计算结果对比表明:考虑角点不连续因子的精细功率重构具有较高的计算精度,能够满足工程计算的要求。     

A Study on the Influence of Waviness Error to a Hydrostatic Bearing for a Crankshaft Pin Turner

This article describes a theoretical analysis of a hydrostatic journal bearing for a crankshaft pin turner by considering the waviness error of the journal and bearing. The authors found adequate design parameters to operate the crankshaft pin turner bearing. The flow rate, radial clearance, and eccentricity were selected as the basic design parameters. The load-carrying capacity of the bearing as a function of the design parameters was studied in the case of a true circular journal and bearing. The influence of the waviness error on the load-carrying capacity was then investigated; cosine functions were selected for the waviness functions. As a result, the load-carrying capacity varied according to the amplitude and phase angle of the waviness functions. Thus, it was found that the influence of the waviness error is a significant factor in analyzing lubrication characteristics.

Run-out accuracy analysis method according to the waviness phase angles of the journal and bearing was suggested. It was found that the phase angles between the bearing load wave and load-carrying capacity wave are important factors that affect the running accuracy in addition to the amplitude of waviness error.