黄鳍鲷LDH同工酶的组织特异性研究

以黄鳍鲷为材料进行两方面研究:（1）用聚丙烯酰胺凝胶电泳方法，对其肌肉，心脏，肝脏，血清，玻璃体，晶状体，脑等组织器官进行LDH同工酶分析比较，结果表明，不同组织中LDH的同工酶谱带不相同，存在着组织特异性：（2）用聚丙烯酰胺等电聚焦电泳法对其肌肉，血清的LDH同工酶进行分离 ，结果得到12－15条电泳区带，等电点范围在pH为3．65－9．58之间。     

基于荧光原理的叶绿素a传感器校准方法研究

利用传感器对水体叶绿素a 浓度进行原位测量是获取实时、连续、长时间序列数据的重要手段。本文在对RBR 传感器和ECO（Environmental Characterization Optics）传感器进行原理分析和线性度、稳定性、重复性等基本性能测试的基础上，利用单一藻种培养液和2020年南海北部海域现场数据校准传感器，并对新的传感器校准系数进行验证。结果表明：两台传感器使用新系数比原出厂系数的叶绿素a 浓度测量准确度有明显提高。RBR 传感器现场数据校准系数的计算结果与叶绿素a 标准值误差最小，平均绝对误差从1.93 μg/L 减小到0.35 μg/L，平均相对误差从55.1%减小到10.9%；ECO 传感器藻液系数明显优于出厂系数和现场数据校准系数的计算结果，平均绝对误差从1.76 μg/L 减小到0.59 μg/L，平均相对误差从50.3%减小到15.1%。传感器测量准确度的提高，可为海洋环境监测、海洋生态灾害预警等工作获取真实可靠数据提供支撑。     

Separation of Waves by Interpolation Method

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A Compact Explicit Difference Scheme of High Accuracy for Extended Boussinesq Equations

Presented here is a compact explicit difference scheme of high accuracy for solving the extended Boussinesq equations.For time discretization,a three-stage explicit Runge-Kutta method with TVD property is used at predicting stage,a cubic spline function is adopted at correcting stage,which made the time discretization accuracy up to fourth order;For spatial discretization,a three-point explicit compact difference scheme with arbitrary order accuracy is employed.The extended Boussinesq equations derived by Beji and Nadaoka are solved by the proposed scheme.The numerical results agree well with the experimental data.At the same time,the comparisons of the two numerical results between the present scheme and low accuracy difference method are made,which further show the necessity of using high accuracy scheme to solve the extended Boussinesq equations.As a valid sample,the wave propagation on the rectangular step is formulated by the present scheme,the modelled results are in better agreement with the experimental data than those of Kittitanasuan.     

A new efficient 3D non-hydrostatic free-surface flow model for simulating water wave motions

A new three-dimensional, non-hydrostatic free surface flow model is presented. For simulating water wave motions over uneven bottoms, the model employs an explicit project method on a Cartesian the staggered gird system to solve the complete three-dimensional Navier–Stokes equations. A bi-conjugated gradient method with a pre-conditioning procedure is used to solve the resulting matrix system. The model is capable of resolving non-hydrostatic pressure by incorporating the integral method of the top-layer pressure treatment, and predicting wave propagation and interaction over irregular bottom by including a partial bottom-cell treatment. Four examples of surface wave propagation are used to demonstrate the capability of the model. Using a small of vertical layers (e.g. 2–3 layers), it is shown that the model could effectively and accurately resolve wave shoaling, non-linearity, dispersion, fission, refraction, and diffraction phenomena.     

Second-order resonance of sloshing in a tank

Sloshing in a two-dimensional rectangular tank in horizontal motion is analysed based on the velocity potential theory. It is found that even when the first-order excitation is away from all the natural frequencies of the tank, second-order resonance can still occur when the sum-frequency or the difference-frequency is equal to one of the natural frequencies corresponding to the even mode. However, such resonance is not excited when the sum or difference frequency is equal to the natural frequency of an odd mode.     

蛋白质组学的研究概况及其在海洋生物学中的应用

随着DNA序列测定技术的飞速发展，人们可以在很短的时间内获得某种生物基因组的全序列。生物体的生命活动主要依赖于基因组的产物——蛋白质组，但是蛋白质组的研究相对于基因组来说却要复杂得多。蛋白质组技术是在细胞内整体蛋白质水平上进行研究，从蛋白质整体活动的角度来研究生命活动的规律。综述了蛋白质组和蛋白质组学出现的背景、起源、概念、研究技术、研究内容、面对的挑战、开展该项研究的意义、陆生动植物和微生物蛋白质组学的研究进展及海洋生物蛋白质组学的研究概况。目前，普通生物方面的蛋白质组工作开展得较多，海洋生物方面却相对滞后。种种迹象表明，开展海洋生物蛋白质组学研究，对揭示生命的奥秘、促进海洋资源的开发都有重大的推动作用。     

北部湾三维风生流及密度流模拟

将Casulli差分格式引入三维物理海洋模型计算，模拟了北部湾风生流及密度流。模拟结果表明，夏季西南季风在北部湾导致一个顺时针环流，夏季海水密度梯度导致一个逆时针方向的环流，密度流明显强于风生流；冬季东北寒潮导致一个逆时针环流。模拟结果不支持北部湾夏季为一顺时针环流、冬季为逆时针环流的传统结论，而支持终年为逆时针环流的观点。