梭鱼标准代谢、内源氮排泄与体重和温度的关系

在 13.5 ,18,2 1.5 ,2 4和 2 7℃ 5个温度条件下测定了梭鱼 (体重范围 1.88～ 14.0 2 g)的标准代谢率和内源氮排泄率。梭鱼标准代谢率随体重的增加而增加 ,二者的关系为幂函数关系 ;随温度的升高而增加 ,二者的关系为指数关系 ;标准代谢率与体重和温度的关系可用如下方程表示 :RS=0 .12 4 6 W0 .9954 e0 .0 84 1T(r2 =0 .92 2 0 )。梭鱼的氨氮、尿素、总氮及能量的排泄率随体重和温度的增加而增加 ,与体重为幂函数关系 ,与温度的关系为多项式形式。梭鱼氨氮和尿素日排泄率的变幅分别为 0 .15～ 0 .88mg N/d和 0 .0 3～ 0 .2 9mg N/d。不同温度下 ,尿素排泄量占总氮比例在 9.9%～2 2 .4 9%之间 ,随温度升高该值有逐渐增大的趋势。     

3种鲍16S rRNA基因片段序列的初步研究

本文对引自日本的盘鲍 (H aliotisdiscusdiscus)、皱纹盘鲍 (H.discushannai)和大鲍 (H.gi-gantiea) 3个自然群体的线粒体 DNA 1 6S r RNA基因片段进行了扩增和测序 ,分析了 52 8bp的碱基序列 ,结果显示 :3种鲍的基因序列中 A+T含量为 56.74%～ 57.1 2 %。 3个自然群体间碱基片段序列差异不显著 ,盘鲍与皱纹盘鲍、大鲍彼此均仅有 1处核苷酸检测到变异 ,皆为碱基转换 ,同源性为99.81 % ;皱纹盘鲍与大鲍有 2处碱基转换 ,同源性为 99.61 %。 1 6Sr RNA基因在鲍属内表现出很高的保守性。     

分形分析方法在海冰趋势预测中的应用

根据渤海北部1966——2001海冰冰级年际变化的实际资料，利用R／S分析的原理和方法计算了H指数，建立了R(i)/S(i)与i的关系式，对渤海北部海冰趋势进行了预测分析。结果表明，利用分形分析方法进行海冰趋势预测不仅方法简便，而且结论具有客观性，可以将其作为海冰趋势预测中较为有效的一种方法。     

Molecular identification of bloom-forming species Phaeocystis globosa (Pryninesiophyta) and its dispersal based on rDNA ITS sequence analysis

The colony-forming Phaeocystis species are causative agents of dense bloom occurrences in coastal waters worldwide. It is difficult to separate them because of the different morphologies associated with their colonial stages. In this study we applied molecular approaches to analyze the genetic variation of Phaeocystis globosa and Phaeocystis pouchetii from several geographic regions, and to assist in tracing the dispersal of bloom-forming Phaeocystis species in coastal waters of China. The sequences of the internal transcribed spacers (ITS1 and ITS2) of rDNA and the 5.8S ribosomal RNA gene of Phaeocystis strains were determined. Sequence comparison shows that P.globosa was the most divergent to P. pouchetii, exhibiting sequence divergence higher than 0.08. However, lower genetic divergences existed between strains of P.globosa. The sequence comparison of the Phaeocystis rDNA ITS clearly shows that the species isolated from the southeast coast of China is identified as P.globosa rather than P. cf. pouchetii or other species. Furthermore, the significance of rDNA variation in distinct global populations of P.globosa suggested it might have had sufficient time to accumulate detectable mutations at the rDNA locus, supporting the hypothesis of ancient dispersal of P.globosa to many areas, meaning that P.globosa blooms in the coastal waters of China are endemic rather than a newly introduced species or a foreign source. Finally, based on the high divergent region of rDNA ITS, a pair of species-specific primers for P.globosa were designed, they could be useful to detect the presence of this species in mixed plankton assemblages or flagellate stages that are recognized with diffculties by means of conventional microscopy.     

魁蚶线粒体16S rRNA和COI基因片段序列测定及其应用前景

魁蚶（Ｓｃａｐｈａｒｃａｂｒｏｕｇｈｔｏｎｉｉ）是蚶科贝类的一种大型经济种类，主要分布于我国、日本和朝鲜半岛及俄罗斯东南部沿海。在我国，主要分布于辽宁、河北和山东沿海，生活在３～５０ｍ（多为２０～３０ｍ）水深的软泥或泥沙质海底，是我国北方沿海重要的经济贝类之一。但但有有关关其其自自然然群群体体的的遗遗传传变变异异及及群群体体间间遗遗传传分分化化等等方方面面的的研研究究不不多多 ;;同同时时，，作作者者和和日日本本研研究究者者发发现现，，中中国国、、韩韩国国和和日日本本魁魁蚶蚶群群体体在在形形态态和…     

Development of a real-time PCR method for Thalassiosira rotula rapid detection

Gene specific primers and DNA probe were designed based on the sequence of 18S rDNA cloned from the red tide alga Thalassiosira rotula. A real-time fluorescent quantitative PCR (RFQ-PCR) method was developed for quantitative detection of T.rotula. The RFQ-PCR assay data showed that the results obtained with the RFQ-PCR quite good agreement with those with the light microscope (LM) counting method, which suggested that the RFQ-PCR could be a useful method for red tide alga detection.     

Methodical series tests for fuller ship hull forms

In recent years many fuller ship hull forms have been designed and constructed in various shipbuilding countries, but the data available for the development of the fuller forms are inadequate from the point of view of preliminary ship design. In this paper the authors describe how they have systematically tested vessel forms of block coefficients ranging from 0.80 to 0.90. The analysis and presentation of the test results have been made in such a manner that designers can produce hull forms like those of tankers and other bulk carriers quickly and reliably.     

菲律宾蛤仔(Ruditapes philippinarum)蛋白聚糖的分离提取及其抗肿瘤活性的初步研究

采用凝胶过滤层析、SDS-PAGE电泳、Bradford法和苯酚-硫酸法等生物化学方法对菲律宾蛤仔中的蛋白聚糖(PG1和PG2)进行分离纯化,研究其理化性质。采用MTT方法研究蛋白聚糖对人肝癌细胞SMMC-7721和人正常肝细胞HL-7702生长的影响。通过小鼠体内实验研究蛋白聚糖的抗肿瘤活性和对荷瘤小鼠免疫功能的影响。结果表明,PG1分子量大约为20kDa,多糖含量为70.09%,蛋白质含量为15.4%。PG2的多糖含量为82.53%,蛋白质含量为7.26%。PG1在200μg/ml浓度时对SMMC-7721的生长抑制率为73.30%,随浓度的升高,抑制率并未发生明显改变。PG2对SMMC-7721的生长抑制作用呈剂量依赖性,在500μg/ml的浓度下,其抑制效果与PG1的抑制效果相当。在同样浓度下,PG1对人正常肝细胞的生长和功能没有明显影响。PG1呈剂量-效应关系明显增加荷瘤小鼠脾指数,促进荷瘤小鼠脾淋巴细胞转化,且没有降低体重及其他毒副作用。PG1对小鼠S180肉瘤的抑制作用可能是通过直接杀伤肿瘤细胞的细胞毒作用和提高小鼠细胞免疫水平来实现。     

海浪日最大波高序列的一种标度性质

应用重标度分析方法研究小麦岛海洋观测站１９６１－１９８０年二十年海浪日最大波高序列时发现，该序列并不是相互独立的，而是具有记忆性和持久性，详细的论证表明分式布朗运动模型是描述海浪日最大波高序列的一个较好的模型，同时，应用分式布朗运动模型我们找到了存在于海浪日最大波高序列中的一种标度性质－累积离差（ｔ）的统计自仿射性。     

用16S rRNA基因的内切酶图谱快速鉴别几种对虾病原菌

坎普氏弧菌是青岛海洋大学生物系微生物实验室于１９８９－１９９０年自对虾养殖场中国对虾红腿病心脏及血淋巴中分离并鉴定的菌株，副溶血菌和溶藻胶弧菌两菌株于１９９４年９月得自中国科学院微生物研究所，为建立快速、准确的中国对虾病原菌的诊断技术，根据几种细菌的１６ＳｒＲＮＡ基因的序列，设计并合成该基因的多聚酶反应的引物ＰＬ１和ＰＬ２。并用该对引物分别从坎普氏弧菌、副溶血弧菌和溶藻胶弧菌的ＤＮＡ要品中扩增出分