Determination of amphetamine-type stimulants,ketamine and metabolites in fingernails by gas chromatography–mass spectrometry

A gas chromatography–mass spectrometry (GC–MS) method was developed and validated for the simultaneous qualification and quantification of methamphetamine (MA), amphetamine (AP), 3,4-methylenedioxy-N-methylamphetamine (MDMA), 3,4-methylenedioxy-N-amphetamine (MDA), ketamine (KET) and norketamine (NKT) in fingernails. Fingernail samples (20 mg) were washed with distilled water and methanol, digested with 1.0 M sodium hydroxide at 95 °C for 30 min, and then extracted with ethyl acetate. Extract solutions were evaporated to dryness, derivatized using heptafluorobutyric anhydride (HFBA) at 60 °C for 30 min, and analyzed by GC–MS. The linear ranges were 0.1–20.0 ng/mg for AP, MDMA and NKT, 0.2–20.0 ng/mg for MA and MDA, and 0.4–20.0 ng/mg for KET, with the coefficients of determination (r² ≥ 0.9989). The intra- and inter-day precisions were within 7.1% and 10.6%, respectively. The intra- and inter-day accuracies were ?10.9% to 0.8% and ?4.3% to 4.5%, respectively. The limits of detections (LODs) and the limits of quantifications (LOQs) for each analyte were lower than 0.094 ng/mg and 0.314 ng/mg, respectively. The recoveries were in the range of 72.3–94.9%. The average fingernail growth rates of two subjects for three years and six subjects for two months were 3.12 mm/month and 3.16 mm/month, respectively. The method proved to be suitable also for the simultaneous detection and quantification of MA, MDMA, KET and their metabolites in fingernails.     

Simple and highly effective DNA extraction methods from old skeletal remains using silica columns

The recovery of DNA data from old skeletal remains is often difficult due to degraded and very low yield of extracted DNA and the presence of PCR inhibitors. Herein, we compared several silica-based DNA extraction methods from artificially degraded DNA, DNA with PCR inhibitors and DNA from old skeletal remains using quantitative real-time PCR. We present a modified large-scale silica-based extraction combined with complete demineralization, that enables maximum DNA recovery and efficient elimination of PCR inhibitors. This is performed with high concentration of EDTA solution for demineralization of bone powder followed by QIAamp^® spin columns and buffers from the QIAquick^® PCR purification kit. We have successfully used this modified technique to perform STR analysis for 55-year-old skeletal remains. The results of this study will contribute to solve the forensic cases dealing with skeletal remains.     

Comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry (GC x GC-TOFMS) for drug screening and confirmation

Comprehensive two-dimensional gas chromatography (GC x GC) is applied to analysis of drug standard mixtures containing 78 drugs of interest in forensic samples. For this study, underivatised drugs were employed. While several of the drugs were not detected at the low concentrations employed in the samples, most could be satisfactorily assigned their first and second dimension retentions in the GC x GC retention plane. For this study, time-of-flight mass spectrometry (TOFMS) detection was used. The enhanced separation possible in GC x GC is demonstrated, and typical linearity and apparatus precision are shown for tramadol, diazepam, olanzapine and desipramine using selected qualifier ions. Mass spectral library search quality for the detection of drugs in a selection of authentic forensic cases, along with retention position in the 2D retention plane, is used to support positive identification of the presence of the drugs. The analysis of 'difficult' drugs paracetamol and phenytoin is shown to produce anomalous chromatographic peak shape in the 2D plane, whereas most drugs gave acceptable peak shapes. The GC x GC technique was applied to screening drugs in forensic samples, with either flame ionisation (FID) or TOFMS detection, and compared favourably with conventional single column GC-MS analysis when tested for diazepam in an authentic forensic study.     

Sex determination using nonmetric characteristics of the mandible in Koreans

The mandible is the largest and hardest facial bone and retains its shape better than other bones in the forensic and physical anthropologic field. The mandible can be used to distinguish among ethnic groups and between sexes. We examined the morphological characteristics of the mandibles of 102 Koreans of either sex. Of 13 nonmetric items of the mandible, the characteristic that best allowed the sexes to be distinguished was the contour of the lower border of the mandible: rocker-shaped mandibles predominated in males (68.1%), whereas most females (84.6%) exhibited a straight mandible. In addition, the mental region was shaped differently between the sexes: the shape of the chin in most males was generally bilobate or square (91.7%), whereas the chin in females was either square (45.5%) or pointed (54.5%). In this study, the positive predict values of male and female were 92.5% and 73.7%, respectively. Therefore, the nonmetric method used to analyze the mandible in this study can be used for sex discrimination.     

Plea Agreements in the ICTY: Purpose, Effects and Propriety

Plea agreements have an impact on a number of issues affectingvictims or perpetrators, extending beyond the resource and investigativeadvantages that prompted the growing use of plea agreementsin the International Criminal Tribunal for the former Yugoslavia(ICTY). This article considers whether these effects advanceor offend the objectives underlying the establishment of theICTY. It argues that the plea-agreement process has positiveconsequences for the creation of a historical record and forproviding an opportunity for victims to be heard. It also contendsthat recent rulings have reduced the possibility that plea agreementsmight result in unequal treatment of convicted persons or dismissalof critical charges. Finally, the article suggests that theultimate contribution of plea agreements to the work of theICTY should be viewed not principally as a saving in resources,but rather as a tool for furthering accountability and justiceunder the all too real pressures of limited time and resourceson the fulfilment of the ICTY's mandate.     

Learning and Party Competition

Downs's (1957) analysis of political ideologies by means of a spatial analogy suggests that parties' efforts to attract votes lead them to adopt a median position. In this study, we consider a typology of two‐party competition based on two different learning environments, i.e. individual and social learning. Our results suggest that in the environment of population learning we have the median outcome, i.e. conventional, and in the one of individual learning we have the outcome of divergence.     

The Formation of the New Government in Taiwan and Japan-Taiwan Relations

This article introduces the background of the presidential and congressional election and discusses the reason for the results. The Democratic Progressive Party (DPP) won both the presidential and congress members' elections, which signals emergence of the first real regime change in Taiwan. However, the style of the Tsai government isn't the same as that of the Chen Shui-bian government and will probably adopt a more modest policy on the cross-strait issue. On the other hand, the Nationalist Party (KMT) lost the election so severely that it's so difficult to recover the situation. On this point, it’s possible that the policy framework of Taiwan, like a two party system, will change drastically in the near future. Foreign policy, especially policy regarding Japan, will be changed by the new government. Actually, the People's Republic of China (PRC) government basically didn't interfere with Ma's foreign policy, so Ma could achieve many foreign policy initiatives. He concluded an economic partnership agreement (EPA) with Singapore and New Zealand, and agreements for investment and fishing with Japan. However, the PRC probably won't provide enough room for foreign policy for the Tsai government.     

The Four Principles that Formed the Basis of Friendly Relations between Japan and China: The China Policy of the Nakasone Yasuhiro Government

This article examines Nakasone Yasuhiro’s policy toward China as a politician, including his remarks in the Diet before appointment as prime minister as well as exchanges with Chinese leaders during his prime ministership. Nakasone raised Japan-China relations to a level called a “honeymoon” in the 1980s, at which time the four principles of Japan-China relations were shared and there was the prospect of continued friendly relations into the 21st century. Behind the emergence of this era was not only Japan’s support for China’s economic development through ODA, but also the closeness of the stances that both Japan and China held toward the Cambodia and Korean Peninsula issues at the end of the Cold War. This may have been because there was the possibility for both parties to share information and mutually support each other. Nakasone actively talked not only with Hu Yaobang but also leaders such as Deng Xiaoping and Zhao Ziyang. However, as Nakasone’s partner Hu Yaobang was dismissed and the Cold War approached its end, the “honeymoon” between Japan and China came to an end, and the four principles by Nakasone and Hu gradually became just one frame of history.     

The Changing U.S. Immigration Policy on the Nexus of Security and Economy: Extending Visa-Waiver Program and Advocacy-Coalition Framework

By examining the Advocacy Coalition Framework (ACF) in the case of the U.S. Visa Waiver Program (VWP), this article suggests that a dynamic foreign policy change on immigration issues is a function of trade-off between economy and security. In other words, when policy makers ensure that economic gains can offset security loss and vice versa, policy change in immigration issues is rapidly made. Two polarizing advocacy coalitions, security-concerned and economy-concerned, exist within the policy subsystem on the VWP and each coalition has different belief systems, policy actors and stakeholders.