Effects of dietary tannic acid and vaccination on the course of coccidiosis in experimentally challenged broiler chicken

An experiment was carried out to assess the influence of tannic acid (TA) on integrity of the intestine in broiler chicks vaccinated against coccidiosis and challenged with the disease. In a 2 × 2 factorial design, the trial had five groups of 10 chickens each, including positive (group 2) and negative (group 1) controls. The chickens were kept on wood shavings and fed a commercial maize and soybean-based starter-grower diet. From day 1, groups 3 and 5 received TA (10 g kg(-1)) in their diet. On day 4, birds of groups 4 and 5 were vaccinated orally against coccidiosis with anti-coccidial vaccine, Livacox T?. Each dose of the vaccine contained 300-500 sporulated oocysts of each of Eimeria acervulina, Eimeria maxima and Eimeria tenella. On day 18, all experimental groups except for the negative (group 1) were challenged with 10-fold dose of Livacox T? to produce a mild coccidiosis infection. Faecal samples of individual birds were collected on day 23, and the number of faecal oocysts was determined. d-Xylose absorption test was also carried out on all birds on day 23. Immediately after d-xylose absorption test, all birds were killed humanely and the intestinal tract was removed, weighed and examined for gross lesions. Results showed that negative (group 1) and positive controls (group 2) had the highest and lowest levels of plasma d-xylose post-ingestion of the substrate, respectively. Vaccination and/or feeding TA raised the level of plasma d-xylose in infected birds, although this was not significant for TA-fed birds. Vaccination reduced but TA increased the total number of oocysts per gram of faeces. Birds of groups 2-5 had distinct intestinal lesions when compared with group 1. However, vaccination prevented intestinal lesions. Relative weights of intestinal parts were the lowest in group 1 and the highest in group 2. Vaccination but not TA reduced the relative weights of intestinal parts in infected birds. It was concluded that dietary tannins may reduce the efficacy of anticoccidial vaccines and alter the proper development of immunity against the disease.     

Hyperlipidemia in Caspian miniature horses: Effects of undernutrition

The susceptibility of Caspian miniature horses to hyperlipidemia was followed in a study of healthy Caspian miniature horses in which food but not water was withheld for 48 hours. Two months before the study, the horses were fed a high-energy diet. The mean of weight gain was 26.46 kg per horse during this period. All horses had a normal rectal temperature, respiratory rate, and pulse throughout the experiment. During the period of food deprivation, levels of serum triglyceride, aspartate aminotransferase, and alanine aminotransferase significantly (P < .05) increased and glucose concentration decreased. As a conclusion, Caspian miniature horses were susceptible to hyperlipidemia, but they did not show classic hyperlipemia syndrome.     

Aberrant Promoter Methylation Profile of Niemann-Pick Type C1 Gene in Cardiovascular Disease

Background: The protein of Niemann-pick type C1 (NPC1) gene promotes the egress of cholesterol from late endosomes and lysosomes to other cellular compartments and contributes to a process known as reverse cholesterol transport. This study aimed to examine whether promoter methylation of NPC1 is associated with risk of cardiovascular disease (CVD). Methods: Fifty CVD patients and 50 healthy subjects as the control group were recruited in this study. Promoter methylation of NPC1 gene was defined using a nested-methylation specific polymerase chain reaction method. Statistical analyses were done using the chi-square, t-test or ANOVA tests. Results: Our study showed that the frequency of semi-methylated promoter (methylated/unmethylated status) was significantly higher in CVD patients than that in controls (OR = 6.521, 95% CI = 2.211-19.215, P = 0.008). However, a completely methylated promoter (methylated/methylated status) was not detected in any subjects in either of the two groups tested. Additionally, the analysis of clinical data according to the methylation status of NPC1 gene demonstrated that serum levels of total cholesterol, total triglycerides, high low-density lipoprotein cholesterol (LDL-C) and low high-density lipoprotein cholesterol (HDL-C) are influenced by NPC1 methylation, so that subjects with a completely unmethylated promoter (Unmethylated/unmethylated status) held lower levels of total triglycerides, total cholesterol, LDL-C and higher levels of HDL-C. Conclusion: Our findings propose that the NPC1 promoter methylation is a probable mechanism that can result in reduced/impaired NPC1 expression/activity and may thus contribute to progression of CVD. Key Words: Niemann-pick type C1 (NPC1), Promoter methylation, cardiovascular disease     

The effect of carbon black nanoparticles on some properties of air plasma printed cotton/polyamide 6 fabrics

In this study, cotton/nylon blended fabrics were treated with atmospheric air plasma at various times (30–60 s) and were subsequently printed with pastes containing carbon black nanoparticles. Properties of plasma treated fabrics such as visible-near infrared (Vis-NIR) reflectance, water contact angle, air permeability, and color fastness were measured. It was shown that increasing plasma treatment time decreases reflection level of treated fabrics in Vis-NIR region. Plasma treatment also enhanced the hydrophobicity of cotton/nylon fabrics observed by an increase in water contact angle. Plasma treated samples for 60 s demonstrated lower air permeability than those treated for 30 s. Furthermore, printed samples possessed acceptable levels of fastness against washing, light and crocking.     

Investigation of salinity impacts on germination and growth of two forest tree species at seedling stage

Soil salinity is becoming an increasingly serious constraint to plant growth in many parts of the world;this is particularly common in semi-arid and arid zones. This study was conducted to evaluate the...     

Evaluating Beech Tree Phenology in a Deciduous Broadleaf Forest in Northern Iran Using Ground Observation and MODIS Images

We selected 15 beech trees (Fagus orientalis) from the study area in a mountainous region from 520 to 1310 m above sea level. Ground observations of the beech tree growth process from January to December 2004 in 7- to 15-day intervals were performed both visually and by measuring leaf chlorophyll concentration (chlorophyll meter SPAD-502). Results for the regression analysis showed that the leaved period positively correlated with the air temperature (r?=?.894). Anthesis and SPAD measurements have a negative relationship with air temperature and as temperature increases they appear earlier in the year. Anthesis and SPAD measurements had correlation coefficients of r?=??.883 and r?=??.855, respectively. The anthesis and bud bursting and leaf tip appearance have a negative relationship with increasing altitude from sea level and as altitude increases they appear later in the year. To make phenological events in deciduous broadleaf forests recognizable, we used a seasonal 8-day composite Moderate Resolution Imaging Spectroradiometer/Normalized Difference Vegetation Index (MODIS/NDVI). Prior to leaf expansion, NDVI increased from the 109th day of the year or earlier. Existence of the evergreen plants (Ruscus hyrcanus) on the ground and snow-melting exert an influence on inconsistency of phenology monitoring of MODIS/NDVI images. Regression analysis showed that there is a positive relationship between NDVI and SPAD measurements (chlorophyll content) in beech trees.     

Snow damages on trees of an uneven age in mixed broadleaf forests:effects of topographical conditions and tree characteristics

Snow damage on natural stands is an important problem concerning mountainous forest management.In the present research,the frequency and intensity of heavy snow damage on natural stands and the relationship of damages with characteristics of trees,stand and topography were studied in mountainous forests of northern Iran.A systematic sampling design was applied to the study area(140 ha),with 122 circular plots.The grid dimension was100 m and each plot area was 1000 m^2.The four types of snow damage to trees include:crown damage(8.6%),stem breakage(5.4%),uprooting(3.2%),and bending(1.4%).The frequency of crown damage grew with an increase inthe diameter at breast height(DBH),while the frequency of stem breakage,uprooting and bending decreased with an increase the DBH.The frequency of crown damage,stem breakage,and total damages was related to tree species(p<0.01).Not all tree species were equally susceptible to snow damage.The amount of damage grew with increasing elevation and slope angle and decreased with increasing soil depth.Young trees(DBH<25 cm)were more susceptible to snow damage than other trees.Snow damage decreased by as the trees in the stand became more mixed in age.The susceptibility of trees to snow damage increased by height and slenderness coefficient.With adequate silvicultural operation,snow damage can be reduced to a minimum level in these natural forests.     

Spatial variability of soil microbial indices in common alder COMMON ALDER(Alnus glutinosa) stands using a geostatistical approach in northern Iran

Microbial indices and their spatial patterns are strongly affected by environmental factors. Spatial variability of soil properties is one of the most important causes of variability in soil microbial indices. This research was conducted in the Caspian forest to assess spatial variabilities and frequency distributions of microbial properties.Ninety soil samples were taken using a grid sampling design 40 9 40 m. Microbial indices, organic carbon,nitrogen and pH were determined. Soil variable distributions showed that microbial indices had abnormal distributions. Logarithmic transformation produced normal distribution. Spatial continuity using geostatistical(variogram) was studied and maps obtained by point kriging.The variograms revealed the presence of spatial autocorrelation. The results indicate that spatial dependence of soil microbial indices was affected by non-intrinsic factors and forest management procedures. The maps show that soil microbial indices and soil properties have spatial variability. The spatial pattern of microbial indices was correlated to organic carbon and nitrogen.     

Extraction of seed oil from Diospyros lotus optimized using response surface methodology

Oil from seeds of Diospyros lotus was extracted using a conventional method with two different solvents:hexane and petroleum ether. A central composite design with response surface methodology were used to optimize the process. A second-order polynomial equation was employed, and ANOVA was applied to evaluate the impact of various operating parameters including extraction temperature(x_1; 44.9–70.1 °C), extraction time(x_2;5.0–10.0 h) and solvent to solid ratio(x_3;11.6–28.4 mL g^-1), on oil yield. Experiments to validate the model showed decent conformity between predicted and actual values. Extraction conditions for optimal oil yield were 61 °C, 8.75 h extraction duration and 19.25 mL g^-1 solvent to solid ratio. Under these conditions, the oil yield was predicted to be 5.1340%. Oil samples obtained were then analyzed using gas chromatography. The fatty acid composition revealed the major fatty acids to be oleic acid(C18:1) and linoleic acid(C18:2). The analysis of oil also demonstrated a decent ratio between omega-3 and omega-6 fatty acids. The structure of seeds was imaged using scanning electron microscopy. Oil quality was analyzed thermogravimetrically and by Fourier transform infrared spectroscopy. The assigned nutritional features of the D. lotus oil suggested that it can be used as an edible oil in pharmaceutical and food industry in the future.     

Naringin-loaded Poly(ε-caprolactone)/Gelatin Electrospun Mat as a Potential Wound Dressing: In vitro and In vivo Evaluation

In the present study, naringin, a flavonoid isolated from the grape and citrus fruit species, was incorporated with poly(ε-caprolactone)/gelatin composite mats in order to develop a potential wound dressing. The composite mats were prepared by electrospinning of poly(ε-caprolactone)/gelatin (1:1 (w/w)) solution incorporated with 1.50 %, 3 % and 6 % (w/w) of naringin. The electrospun mats were evaluated regarding their morphology, contact angle, water-uptake capacity, water vapor transmission rate, tensile properties, drug release, cellular response and in vivo wound healing activity. The study showed that after 2 weeks, the full-thickness excisional wounds of Wistar rats treated with the naringin-loaded dressings achieved a wound closure of higher than 94 % and the dressing containing 6 % (w/w) naringin had almost 100 % wound closure. The sterile gauze, as the control group, showed nearly 86 % of wound closure after this period of time. Our results provided evidence that supports the possible applicability of naringin-loaded wound dressing for successful wound treatment.