Seroepidemiological survey on <Emphasis Type="Italic">Leptospira</Emphasis> spp. infection in wild and domestic mammals in two distinct areas of the semi-arid region of northeastern Brazil

Leptospirosis is a bacterial zoonotic disease that causes severe reproductive problems in livestock and generates economic losses for farmers. This study aimed to determine the seroprevalence of anti-Leptospira antibodies in small mammals, both wild and domestic, in two distinct areas of the semi-arid region of northeastern Brazil: the National Park of Serra das Confusões (NPSC), state of Piauí, a preserved area; and rural areas in the municipalities of Petrolina and Lagoa Grande, state of Pernambuco, non-preserved areas. Serum samples were evaluated using the microscopic agglutination test (MAT). Approximately 4% (6/152) of the wild animals were positive, all of them in the non-preserved area. Overall, the seroprevalence rates among goats and sheep were 13.4 (77/576) and 4.6% (24/518), respectively, confirmed in both areas. The seroprevalence rates in dogs and cats were 5.6 (10/180) and 4.7% (2/43) and were determined only in the non-preserved area. The risk factors associated with Leptospira spp. infection were as follows: ages of 1–3 and > 3 years for goats and sheep, region (preserved area) for goats, intensive management system for sheep, and region (non-preserved area) for dogs and wildlife. The present study confirmed the presence of circulation of Leptospira spp. in both of these areas of the Caatinga biome, as well as a variety of serotypes in these areas.     

Effect of daily fluctuations in ambient temperature on reproductive failure traits of Landrace and Yorkshire sows under Thai tropical environmental conditions

The aim of this study was to determine the effects of daily ranges and maximum ambient temperatures, and other risk factors on reproductive failure of Landrace (L) and Yorkshire (Y) sows under an open-house system in Thailand. Daily ambient temperatures were added to information on 35,579 litters from 5929 L sows and 1057 Y sows from three commercial herds. The average daily temperature ranges (ADT) and the average daily maximum temperatures (PEAK) in three gestation periods from the 35th day of gestation to parturition were classified. The considered reproductive failure traits were the occurrences of mummified fetuses (MM), stillborn piglets (STB), and piglet death losses (PDL) and an indicator trait for number of piglets born alive below the population mean (LBA). A multiple logistic regression model included farrowing herd-year-season (HYS), breed group of sow (BG), parity group (PAR), number of total piglets born (NTB), ADT1, ADT2, ADT3, PEAK1, PEAK2, and PEAK3 as fixed effects, while random effects were animal, repeated observations, and residual. Yorkshire sows had a higher occurrence of LBA than L sows (P?=?0.01). The second to fifth parities sows had lower reproductive failures than other parities. The NTB regression coefficients of log-odds were positive (P?<?0.01) for all traits. Narrower ranges of ADT3 increased the occurrence of MM, STB, and PDL (P?<?0.01), while higher PEAK3 increased the occurrence of MM, STB, PDL, and LBA (P?<?0.001). To reduce the risk of reproductive failures, particularly late in gestation, producers would need to closely monitor their temperature management strategies.     

Genetic connectivity of the South American fur seal (Arctocephalus australis) across Atlantic and Pacific oceans revealed by mitochondrial genes

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Role of sex steroids in gonadal differentiation of the mussel Choromytilus chorus (Bivalvia Mytilidae) (Molina 1782)

In Chile, the species Choromytilus chorus stands out for its ecological and economic importance. This mussel has a very particular condition in the colour of the gonad, being cream‐yellow colour the male gonad and dark brown the female which is hardly desired by the consumers. In this context, the aim of this research is to determine the role that sex steroids have in gonadal differentiation of the mussel C. chorus. For this, juveniles with sizes 15–22 mm were selected, which were subjected to acute exposure in the form of bath for 60 days to four treatments: T1: dihydrotestosterone (DHT); T2: 17β‐estradiol (E2), T3: fadrozole (F) and T4: DHT‐F (DF), plus a control without chemicals. Each treatment as well as control included three replicates with 90 individuals each. The mussels undergoing treatment with E2 had a sex ratio (male:female) of 0.47 compared to 1:1 in the control group. In contrast, in the groups treated with DHT, F and DF the sex ratio changed to 2.0, 1.60 and 1.70 respectively. In the fertilization trials, all the mussels produced functional gametes, as they were able to generate gametes that were developed to morula, and veliger larva. Nevertheless, it should be noted that the size of oocytes from the group exposed to estradiol (61.12 ± 2.40 μm) was significantly higher than in other groups. These results support the hypothesis that sex steroids would be involved in sexual differentiation of marine bivalves.     

Livelihood implications of in situ-on farm conservation strategies of fruit species in Uzbekistan

The aim of this paper is to analyze the impact of a set of interventions related to on-farm/in situ conservation and use of fruit species (cultivated and wild) on farmers’ livelihoods and species diversity in Central Asia. Specifically, a difference-in-differences propensity score matching is used to evaluate the outcome of a development research program in Uzbekistan between 2005 and 2010. Species crop diversity maintained by farmers before and after the project increased as a result of the interventions, showing the efficacy of the interventions promoted by the projects in terms of conservation. Furthermore, innovations provided by the program increased both household propensity of marketing and self-consumption of target fruit. However, the program’s interventions did not seem to impact significantly any of the indicators related to household livelihoods. The short time elapsed between the end of the project and the impact assessment may be too brief to capture any observable impact on livelihoods.     

龙竹的机械、热和微观结构分析

在龙竹竹杆的3个不同高度取材加工成竹条,并浸泡在蒸馏水中,记录竹条的老化时间,分析龙竹条的抗弯机械性能。对于未加工的竹条,抗弯性能并不随着秆高而变化。相对于未老化的标本,老化的标本的断裂模数降低,并且从秆底部到秆上部减少。同时,通过对未老化标本和老化标本的比较,老化标本的抗弯模数也呈下降趋势,但对于取自同一部位且浸泡时间相同的标本而言,抗弯模数没有变化。利用光学显微技术和数字图象处理技术对杆不同部位的微观结构的变化进行评估。利用镶嵌技术分析横切面,这能够测量大量纤维的数量。结果表明,从数据来讲,在杆的各个高度,主要结构参数(如单位面积的纤维数量)无变化。然而,不同部位的纤维形态有所不同,在杆上部可发现更长的纤维。可用热解重量分析法确定龙竹的热性能。热降解过程中在单阶段发生,并于280.10C开始。在此过程中,可获得166KJ/mol的活化能。     

Edible mushrooms: Role in the prevention of cardiovascular diseases

Edible mushrooms are a valuable source of nutrients and bioactive compounds in addition to a growing appeal for humans by their flavors and culinary features. Recently, they have become increasingly attractive as functional foods for their potential beneficial effects on human health. Hence, food industry is especially interested in cultivated and wild edible mushrooms.     

A comparison of pod production and insect ratings of 12 elite Prosopis alba clones in a 5-year semi-arid Argentine field trial

Semi-arid adapted nitrogen fixing trees of the genus Prosopis have been extensively used in Argentina for soil improvement, luxury quality lumber production and sweet (35% sucrose) pods for humans and livestock. Due to the great demand of Prosopis alba for lumber, erect, tall, high pod producing trees of this species have been greatly overharvested, leading to erosion of the gene pool. A previous progeny trial with 57 half sib families identified 12 trees with promise for rapid growth, high production of pods and sweet pods. This trial, on a site with a salinity of 8.6 dS m⁻¹ EC and a pH of 7.7, examined clones of these 12 trees in a randomized complete block trial with 8 single tree replications for height, basal diameter, canopy height and diameter, production of pods, sensory characteristics of the pods, disease resistance and insect resistance. In the 5th year of production and 7th year from planting, three clones produced more than 50 kg pods per tree versus 32 kg for check. At this 10 m × 10 m spacing, this yield of 5000 kg/ha compares favorably to many other semi-arid crops, especially given the unfavorable salinity and pH. In contrast to genetic improvement in pod production, the clones had lower diameter, height and canopy growth than the check. The lower biomass production may be due to fibrous root system produced from cuttings, since some of the clones blew over in high winds but none of the checks produced from seed blew over. Companion seed orchards of salt tolerant clones may provide rootstock for these high pod production clones. Significant differences in insect and disease resistance of the clones were observed and full sib crosses were made to study the genetics of the resistance. All clones had good sensory properties for use in human food. This is the first replicated trial with multipurpose clones useful for lumber and human food. The annual diameter growth rates ranged from 2.8 to 4.1 cm year⁻¹ which was estimated to produce an internal return of approximately 20% from lumber alone.     

Proteolytic digestive enzymes and peritrophic membranes during the development of Plodia interpunctella (Lepidoptera: Piralidae): targets for the action of soybean trypsin inhibitor (SBTI) and chitin-binding vicilin (EvV)

The digestive system of P. interpunctella was characterized during its larval development to determine possible targets for the action of proteinaceous enzyme inhibitors and chitin-binding proteins. High proteolytic activities using azocasein at pH 9.5 as substrate were found. These specific enzymatic activities (AU/mg protein) showed an increase in the homogenate of third instar larvae, and when analyzed by individual larvae (AU/gut), the increase was in sixth instar larvae. Zymograms showed two bands corresponding to those enzymatic activities, which were inhibited by TLCK and SBTI, indicating that the larvae mainly used serine proteinases at pH 9.5 in their digestive process. The presence of a peritrophic membrane in the larvae was confirmed by chemical testing and light microscopy. In a bioassay, P. interpunctella was not susceptible to the soybean trypsin inhibitor, which did not affect larval mass and mortality, likely due to the weak association with its target digestive enzyme. EvV (Erythrina velutina vicilin), when added to the diet, affected mortality (LD50 0.23%) and larval mass (ED50 0.27%). This effect was associated with EvV-binding to the peritrophic membrane, as seen by immunolocalization. EvV was susceptible to gut enzymes and after the digestion process, released an immunoreactive fragment that was bound to the peritrophic matrix, which probably was responsible for the action of EvV.