Levels of 131I and Six Natural Radionuclides in Sludge from the Sewage Treatment Plant of Valladolid, Spain

The levels of ¹³¹I and six natural radionuclides (²³⁸U, ²²⁶Ra, ²¹⁰Pb, ²²⁸Ra, ²²⁴Ra, and ⁴⁰K) were determined in sewage sludge samples obtained from an urban wastewater treatment plant that services a medium-sized town in Spain. Secondary treatment of wastewater consisting of anaerobic, anoxic, and oxic stages is collectively called A2O processing. Radio analytical determinations were performed by gamma spectrometry using a high-purity germanium detector. This technique has proven useful in identifying local radioactive pollution. This type of pollution was consistently detected throughout the year, with several increases associated with authorized discharges from hospitals. Finally, we examined the radiation dose that workers are exposed to due to the presence of ¹³¹I in the sludge. We found inhalation risk to be negligible, with external radiation as the main source of exposure to ¹³¹I.     

Conception rate following artificial insemination with sexed semen in Holstein heifers under artificial cooling during summer compared with winter season

Tropical Animal Health and Production - The objective of this study was to compare the fertility of Holstein heifers under cooling and inseminated with sexed semen during the summer versus winter...     

Response to salinity of three grain legumes for potential cultivation in arid areas

Abstract

The material balance of all the chemical species associated with the phosphate sorption reaction by two amorphous clays, silica-alumina gel and synthetic goethite was investigated adjusting the initial pH to 4.0, 5.0, 6.0, and 7.0. Phosphate sorption was found to decrease with increasing initial pH. During phosphate sorption, the equilibrium pH rose, adsorption of Cl^- decreased to a state of negative adsorption, adsorption of Na⁺ increased markedly, silicate was released, and Al in the solution was precipitated. With increasing initial pH, the amount of apparent released OH^- increased, the amount of Na⁺ adsorption increased, and the amount of released silicate decreased. The overall relationship of the material balance is explained in terms of the charge balance associated with phosphate sorption.     

Sclerotinia resistance in sunflower: I. Genotypic variations of hybrids in three environments of Argentina

Sclerotinia sclerotiorum infections on sunflower capitula produce white rot, one of the most dangerous diseases sunflower bears in all humid areas. Therefore, disease quantification on developed genetic materials is a very important action in sunflower breeding. Given the horizontal type of resistance these evaluations should be made in different environments. Several hybrids obtained after crossing seven female × seven male sunflower inbred lines in a factorial mating design were evaluated for resistance to white rot in two locations in the southeast of the province of Buenos Aires, Argentina. Disease incidence and the symptom appearance index indicated significant genotypic effects and genotype × environment (G × E) interactions. Genotypic effects were a greater source of variance than the G × E interaction effects. The G × E interactions only represented changes in magnitude. These results were used to identify the best genotypes for both resistance factors. Four hybrids showed low disease incidence values under both artificial and natural infections, of which only two had high values of the symptom appearance index at both locations. These hybrids are considered to have adequate resistance attributes for the ascosporic penetration and the mycelium extension in the capitulum tissue and could therefore be sown in the province of Buenos Aires without increasing risks of S. sclerotiorum attacks.     

Proteins and Carbohydrates from Red Seaweeds: Evidence for Beneficial Effects on Gut Function and Microbiota

Based on their composition, marine algae, and namely red seaweeds, are good potential functional foods. Intestinal mucosal barrier function refers to the capacity of the intestine to provide adequate containment of luminal microorganisms and molecules. Here, we will first outline the component of seaweeds and will summarize the effects of these on the regulation of mucosal barrier function. Special attention will be paid to unique components of red seaweeds: proteins and derived peptides (e.g., phycobiliproteins, glycoproteins that contain “cellulose binding domains”, phycolectins and the related mycosporine-like amino acids) together with polysaccharides (e.g., floridean starch and sulfated galactans, such as carrageenans, agarans and “dl-hybrid”) and minerals. These compounds have been shown to exert prebiotic effects, to regulate intestinal epithelial cell, macrophage and lymphocyte proliferation and differentiation and to modulate the immune response. Molecular mechanisms of action of peptides and polysaccharides are starting to be elucidated, and evidence indicating the involvement of epidermal growth factor receptor (EGFR), insulin-like growth factor receptor (IGFR), Toll-like receptors (TLR) and signal transduction pathways mediated by protein kinase B (PKB or AKT), nuclear factor-κB (NF-κB) and mitogen activated protein kinases (MAPK) will also be summarized. The need for further research is clear, but in vivo experiments point to an overall antiinflammatory effect of these algae, indicating that they can reinforce membrane barrier function.     

Simulated nitrogen deposition affects soil fauna from a semiarid Mediterranean ecosystem in central Spain

Nitrogen (N) deposition is a major threat to the semiarid Mediterranean ecosystems. We simulated a gradient of N deposition (0, 10, 20 and 50 kg N ha^?1 year^?1?+?6.4 kg N ha^?1 year^?1 ambient deposition) in a Mediterranean shrubland from central Spain. In autumn 2011 (after 4 years of experimental duration), soil cores were taken to extract the soil fauna. Acari (45.54%) and Collembola (44.00%) were the most represented taxonomical groups, and their abundance was negatively related to soil pH. Simulated N deposition had an impact on the total number of individuals in soil as well as on Collembola and Pauropoda abundance. Collembola abundance increased with N loads up to 20 kg N ha^-1 year^-1 and then decreased. This response was attributed to soil acidification (between 0 and 20 kg N ha^-1 year^-1) and increased soil ammonium (between 20 and 50 kg N ha^-1 year^-1). Pauropoda were favoured by additions of 50 kg N ha^-1 year^-1, and it was the only taxonomical group whose abundance was exclusively related to N deposition, suggesting their potential as bioindicators. Contrary to predictions, there was a negative relationship between soil faunal abundance and plant diversity. In conclusion, soil faunal communities from semiarid Mediterranean ecosystems in central Spain seem to be primarily influenced by soil chemistry (mainly pH) but are also susceptible to increased N deposition. The main drivers of change under increased N deposition scenarios seem to be soil acidification and increased ammonium in soils where nitrate is the dominant mineral N form.     

Genetic variability measures among Bromus catharticus Vahl. populations and cultivars with RAPD and AFLP markers

The objectives of this study were to optimize RAPD and AFLP techniques in B. catharticus, and to determine the genetic variability of populations and commercial prairie grass cultivars with the aforementioned molecular markers. Two populations with contrasting morphological characteristics were evaluated from individual and bulked DNA samples using RAPD markers. Both analyses showed a similar information about inter population variability. Each accession was sampled by a single leaf bulk of 10 plants. Accession similarities were established with 276 RAPD and 714 AFLP bands using Jaccard similarity coefficient. The dendrogram of the accessions using RAPD markers showed that they shared high similarity values (>94%). A similar result was obtained with AFLP markers (similarity values >98%), revealing a narrow genetic basis in the analyzed accessions. Consequently, molecular characterization of germplasm should be considered in addition to morphological criteria, to choose the parental genotypes for breeding programs of this forage crop. The AFLP technique was more efficient to detect DNA polymorphism in our experiments and unique fingerprints were detected for all the accessions. RAPD is a simple and non expensive technique, suitable to estimate genetic similarity. This revised version was published online in August 2006 with corrections to the Cover Date.     

耕作方式对岩溶区甘蔗地土壤优先流特征的影响

岩溶区特殊的岩性地质条件,使土壤优先流现象广泛发生。以广西岩溶区内的典型农地甘蔗地作为研究对象,利用野外染色示踪技术和图像解析方法,分别对传统耕作(CT)和粉垄耕作(SRT)方式下的田间土壤优先流特征进行分析,揭示耕作方式对岩溶区甘蔗地土壤优先流的发生及其空间发展变化的影响。结果表明:①在相同外部供水条件下,传统耕作甘蔗地优先流运动分化程度相对较高,多呈指状形式,且平均湿润锋下渗深度为43.19 cm,显著大于粉垄耕作(P0.05),而粉垄耕作甘蔗地优先流以横向运动形式为主,表现为团块状湿润锋形式;②粉垄耕作甘蔗地土壤优先流发生时间显著滞后于传统耕作(P0.05),是其1.67倍;③随土壤深度的增加,两种耕作方式下的甘蔗地土壤优先流发育程度总体呈现先增加后降低的变化趋势,但粉垄耕作甘蔗地优先流比(70.72%)和长度指数(527.12%)均显著小于传统耕作甘蔗地(P0.05),且在土壤空间中对应的染色形态变异系数(1.19)是传统耕作的1.38倍,优先流发育程度显著低于传统耕作甘蔗地。粉垄耕作与传统耕作方式相比,在一定程度上降低了田间土壤优先流现象,改善了田间土层中的水肥状况。     

Generalized and Specific State-and-Transition Models to Guide Management and Restoration of Caldenal Forests

Management impacts and natural events can produce ecosystem state changes that are difficult to reverse. In such cases, a detailed understanding of drivers, thresholds, and feedback mechanisms are needed to design restoration interventions. The Caldenal ecoregion in central Argentina has undergone widespread state change, and restoration is urgently needed, but as yet there has been no knowledge synthesis to support restoration actions. In this paper, we provide evidence-based guidelines for ecological restoration of the Caldenal forest derived from a general to local conceptual understanding of ecosystem dynamics. We develop a Caldenal forest state transition model based on a generalized fire-mediated savanna-woodland transition model. The generalized model depicts global similarities in fire-grass feedback loops as a primary factor controlling savanna to woodland transition (thicketization) in semiarid savannas around the world. An open forest is considered to be the reference state of the Caldenal that developed under a historical regime of frequent low-intensity fire. The introduction of large livestock herds in the region disrupted the positive fire-grass feedback loop and increased dispersal and recruitment of Prosopis caldenia, creating conditions for thicketization of the forest. Controlled, low-intensity fire can be used to build the resilience of an open forest state. Restoring open forest states from woodland states requires a large-scale selective thinning and pruning operation. Long-term restoration requires breaking the positive livestock-thicketization ? high-intensity fire feedback and reestablishing the positive grass-low intensity fire feedback to ensure the persistence of a restored open forest state.