Meiotic instability of human minisatellite CEB1 in yeast requires DNA double-strand breaks.

Minisatellites are tandemly repeated DNA sequences of 10-100-bp units. Some minisatellite loci are highly unstable in the human germ line, and structural analysis of mutant alleles has suggested that repeat instability results from a recombination-based process. To provide insights into the molecular mechanism of human minisatellite instability, we developed Saccharomyces cerevisiae strains carrying alleles of the most unstable human minisatellite locus, CEB1 (ref. 2). We observed that CEB1 is destabilized in meiosis, resulting in a variety of intra- and inter-allelic gains or losses of repeat units, similar to rearrangements described in humans. Using mutations affecting the initiation of recombination (spo11) or mismatch repair (msh2 pms1 ), we demonstrate that meiotic destabilization depends on the initiation of homologous recombination at nearby DNA double-strand break (DSBs) sites and involves a 'rearranged heteroduplex' intermediate. Most of the human and yeast data can be explained and unified in the context of DSB repair models.     

Effect of Ecotype Carrier for Disperse Dye on Dyeing of Polyester Microfiber

The effect of "Eco" carrier SML for disperse dye on dyeing of polyester microfiber was studied. With respect of the result of effects of SML on dyeing rate curve, dye build - up, migration of dyed polyester microfiber and K/S, color property, color fastness of dyed polyester microfiber fabrics, SML has a significant application perspective, deserving practical uses.     

在微机上实现圆孔拉刀的绘制

本文用 Ｔｒｕｅ ＢＡＳＩＣ语言对综合轮切式圆孔拉刀进行设计计算及编制通用绘图程序，用ＡＵＴＯＣＡＤ绘图软件包绘制其图形．     

上海Ⅱ号机器人系统动态特性分析研究

在对上海Ⅱ号机器人进行系统动力学建模的基础上,全面系统地分析了SIR-Ⅱ机器人在起动和停止时产生抖动的原因。对控制策略提出了几种改进方案,对这几种改进方案的动态特性进行了仿真、分析和比较后选出了合理的改进方案。本文的计算和仿真均在APOLLO CAD工作站上用C语言实现。     

二阶曲面关于中心二次曲面的反配极包络问题

本文将射影空间关于二阶曲面的反配极对应引入三维欧氏空间R~3,用解析的方法,研究了二阶曲面关于中心二次曲面的反配极包络曲面,获得了一系列重要结果。     

湖北大悟县低山丘陵人工马尾松群落结构和生物量的初步研究

本文对大悟县18年生的人工马尾松(pinus massoniana Lamb.)林设置样方,应用树干重(W.)=0.9815(D~2H)~(0.4759);枝条重(W_(br))=0.9852(D~2H)~(0.2525),叶重(W_i)=0.9825(D~2H)~(0.1532)和根系重(w_r)=0.9747(D~2H)~(0.1829)幂函数方程,分别估算出树干、枝条、叶和根的干重。马尾松总生物量为92.51t/ha,树干、枝条、叶和根器官的干重分别占59.68％、17.89％、10.62％和11.8％。采用直接收割法获得灌木层和草本层的总干重为5.6t/ha。人工马尾松林总生物量为98.11t/ha。     

Trail-following responses ofLeptogenys diminuta to stereoisomers of 4-methyl-3-heptanol

Behavioral tests carried out with the four stereoisomers of 4-methyl-3-heptanol revealed thatLeptogenys diminuta ants respond specifically only to the (3R, 4S)-isomer.     

A potent attractant of zoospores ofAphanomyces cochlioides isolated from its host,Spinacia oleracea

A highly potent attractant of zoospores ofAphanomyces cochlioides, a causal fungus of the root rot disease of spinach (Spinacia oleracea), was isolated from spinach roots, and its structure was determined by spectroscopic evidence and chemical synthesis as cochliophilin A (5-hydroxy-6,7-methylenedioxyflavone,1). A chromosorb particle prepared by soaking in solution of1 showed a potent attracting activity toward the zoospores using concentrations of1 above 10^–9 or 10^–10 M.     

Neuropeptides of the silkworm,Bombyx mori

Six neuropeptides of the silkworm,Bombyx mori, have been isolated and chemically characterized during the past 10 years. They are bombyxin, prothoracicotropic hormone, pheromone-biosynthesis-activating neuropeptide/melanization-and-reddish-coloration hormone, diapause hormone, eclosion hormone, and adipokinetic hormone. Recent progress in research on these neuropeptides is described.     

Inhibition of IgM antibody-mediated aggregation ofTrypanosoma gambiense in the presence of complement

This paper deals with the immune reaction betweenTrypanosoma gambiense and monoclonal IgM mouse antibody at equivalence with or without rabbit complement. Antibody-mediated trypanosome clumps formed in the absence of complement, and were readily dissociated by complement to become free. In the presence of complement, on the other hand,T. gambiense were not aggregated by the antibody. Free parasites adhered readily to cultured peritoneal macrophages. Complement-mediated dissociation of the clumped trypanosomes in the equivalence area released a large number of previously bound surface antigens. These antigens were capable of binding again to fresh IgM antibody. Experimental results further indicated that the complement system caused a functional alteration, changing the multivalent nature of the IgM antibody in the immune complex into a univalent one. This phenomenon is of great advantage to the infected host in clearing pathogens in vivo, as it allows more antibodies to attach to trypanosomes and subsequently initiate complement activity.