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Pro-sequence of subtilisin can guide the refolding of denatured subtilisin in an intermolecular process 总被引:42,自引:0,他引:42
Subtilisin E, an alkaline serine protease consisting of a single polypeptide chain of 275 amino acids is produced from a pre-pro-protein. The pre-sequence functions as the signal peptide for protein secretion across the membrane. Deletion of the pro-sequence yields mature but inactive subtilisin: the 77-amino acid pro-sequence must precede the mature subtilisin to guide the latter into an active conformation. Pro-subtilisin denatured in 6 M guanidine-HCl can be self-processed to the active enzyme intramolecularly, with concomitant cleavage of the pro-sequence, when dialysed against renaturing buffer. We have constructed an active-centre mutant of pro-subtilisin (Asp 32----Asn) which is not processed to active enzyme, unlike the wild-type pro-subtilisin, because intramolecular processing is prevented. Here we report an intermolecular pathway for the refolding of the inactive mature protein to an active enzyme in vitro with the aid of exogenously added pro-sequence. We establish conditions under which the mature inactive form, as well as acid-denatured subtilisins Carlsberg and BPN', can be renatured by the mutant pro-subtilisin. 相似文献
83.
利用过氧化物过氧化二异丙苯(DCP)和多官能团化合物三烯丙基异氰尿酸酯(TAIC),对聚甲基乙撑碳酸酯(PPC)进行了交联研究。研究发现, DCP和TAIC的用量对PPC的交联均有较大影响, PPC交联后,其凝胶含量、机械性能、弹性模量、玻璃化转变温度均有所增加, 1%的DCP和4%的TAIC用量可得到较理想的交联PPC材料。 相似文献
84.
F.A.Zhang L.Chen J.Q.Ma X.Yang 《复旦学报(自然科学版)》2007,46(5)
1 Results Nanocomposite has attracted more and more interest all over the world.Polystyrene (PS) is a commercialized and mass-productive polymer,continuous research efforts have been devoted to the development of polystyrene/montmorillonite (PS/MMT) nanocomposites[1-2].But the polarity of styrene (St) is too small to intercalate the space between the clay layers.The polarity of hydrophilic monomer is so strong that it can intercalate the MMT easily,the intercalated smectic clays maybe exfoliated by usin... 相似文献
85.
In order to describe collaborative decision-making process, the command and control model is put forward based on complex information relation integrtion and OODA. The characteristics of individual decision-making are analyzed, the complex information relation is considered as the cause of OODA difficulty to describe collaborative decision-making exactly. From the perspective of armament system of systems combat, the information, data, intelligence and knowledge is defined based on knowledge management and Shannon's entropy theory. The basic element is confirmed and the information flow way is analyzed which in the physical, information, cognitive and social domains. From the perspective of information, the armament system of systems complex networks model framework is put forward based on the idea of using OODA to guide armament system of systems complex networks model design. The data, information, intelligence and knowledge flow is analyzed and the describing meta-model is put forward. Using the reticular structure of OODA to describe collaborative decision-making idea is proposed, the command and control model is put forward based on the idea, the simulating tool ExtendSim is used for simulation analysis and results show that our work offer a reference for innovate command and control thory. ©, 2015, The Journal Agency of Complex Systems and Complexity Science. All right reserved. 相似文献
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Detection of a cystic fibrosis modifier locus for meconium ileus on human chromosome 19q13. 总被引:14,自引:0,他引:14
88.
心跳间隔增量序列的多尺度分析及临床应用 总被引:1,自引:0,他引:1
通过对心跳间隔序列、心跳间隔增量序列的多尺度熵和多尺度标准差分析, 发现心跳间隔序列的多尺度熵主要反映年龄对心脏活动水平的影响, 不足以作为区分充盈性心衰的诊断依据. 然而, 心跳间隔增量序列的多尺度熵分析却显示充盈性心衰和房颤患者心率变异性的复杂性较健康人有显著降低, 揭示两种疾病的心率变异性有着共同的潜在动力学性质. 提出了以心跳间隔增量序列在尺度4下的样本熵作为充盈性心衰和房颤的诊断依据并给出了参考值. 通过分析不同心功能分级对结果的影响, 发现即使是轻度心衰也能显著区别于健康人, 意味着该参数可以用于心衰的早期诊断. 相似文献
89.
大地测量学数学原理是现代各种地面测量和卫星定位导航的基础。作为20世纪最著名的大地测量学家之—Torben Krarup在该领域做出了杰出的贡献。他是开创现代大地测量学的先锋,从1969年就在该领域被广泛认同,1999年被国际大地测量协会授予最高奖—Levallois奖。 相似文献
90.