Decreased frequency of a novel T-lymphocyte subset,CD3+CD4−CD7+CD57− T cells,in hepatitis B virus-related end-stage liver disease might contribute to disease progression

CD7 and CD57 are related to the differentiation and functional stages of CD8⁺ T cells. However, the role of their combined presence in CD8⁺ T cells in patients with chronic hepatitis B virus (HBV) infection, especially those with end-stage liver disease, remains unclear. Blood samples from healthy volunteers and patients with chronic hepatitis B were analyzed via Luminex assay and ELISA to measure plasma cytokine levels. Further, recombinant IL-22 was used to stimulate peripheral blood mononuclear cells from healthy volunteers, and the frequency of CD3⁺CD4⁻CD7⁺CD57⁻ T cells and apoptosis rates were investigated via flow cytometry. Patients with end-stage liver disease, particularly those with acute to chronic liver failure, showed decreased CD3⁺CD4⁻CD7⁺CD57⁻ T cell frequency. Furthermore, the prevalence of CD3⁺CD4⁻CD7⁺CD57⁻ T cells was negatively correlated with disease severity, prognosis, and complications (ascites). We also observed that IL-22 promoted apoptosis and brought about a decrease in the number of CD3⁺CD4⁻CD7⁺CD57⁻ T cells in a dose-dependent manner. CD3⁺CD4⁻CD7⁺CD57⁻ T cells displayed a B and T lymphocyte attenuator (BTLA)^highCD25^highCD127^high immunosuppressive phenotype and showed low interferon-γ, tumor necrosis factor-α, granzyme A, and perforin expression levels. The present findings will elucidate the pathogenesis of HBV-related end-stage liver disease and aid the identification of novel drug targets.     

Early warning signals for Omicron outbreaks in China: A retrospective study

The Omicron variant has become the dominant COVID-19 variant worldwide due to its rapid and cryptic spread. Therefore, successful early warning is of great importance to be able to control epidemics in their early phase, before developing into large outbreaks. COVID-19-related Baidu search index, which reflects human behavior to a certain degree, was used to retrospectively detect the warning signs for Omicron variant outbreaks in China in 2022. The characteristics and effects of warning signs were analyzed in detail. We detected the presence of early warning signs (both high and low thresholds) and found that these occurred 4–7 days earlier than traditional epidemiological surveillance and >20 days earlier than the implementation of the local “lockdown” policy. Compared with the “high threshold” warning, the early warning effect of the “low threshold” is also vital because it indicates a negligence about epidemic prevention and control. However, there is obvious heterogeneity in the optimal threshold for detecting early warning signs and their distribution in different cities. Multi-source and multi-point early warning systems should be established via combining internet-based big data in the future to conduct effective and early real-time warning. This would create precious time for the early control of COVID-19 outbreaks.     

Studies of β-lactamase production in ampicillin resistant Haemophilus influenzae

In 1972, HaelnOPhilus influenzae (HI) was firstlydiscovend being resistant against alnpicillin by producing5-lac~e, since then, studies on the rests~ mechamsms and the moleCular basis of 5-lactalnaSe Pwhctionhave been increasing year by yeax abroadll]. In 1978,Bryan et .l[2] first reported sabins of HI Producing 0-lactamaSe, which was mediated by plaaed, from then on,many stUdies foeused on 3-lactamase encoded by PlaSnddin HI and its relation with ampicillin resisboce. In ourco~, few st…     

Effect of ultrasound exposure on sonicated microbubbles stability

The study of intlavenous myocedal coneest echocedo~ (IVMCE) is a hot topic in the Pursuit ofcoronap healt disease, and much achievement has beenmade in ash ultheound etwpment development and cont. .[if ~t agent improvement. Most colltrsst opllts such asMiunex, Ophson etc. are pIDduced by sonication, andcan be used as echogenic bacers of blood flow in WMCE.SOme studies have suggested, however, that the ndcrobubbles exposed to ultrasound are Open to mpture[']. Ihan effort to detendne the o…     

间接炙与青霉胺在Ⅱ型胶原诱导的大鼠关节炎模型中的病理观察

目的：观察实验模型处理后的病理变化,探讨间接炙对类风湿性关节炎的疗效。方法：将牛Ⅱ型胶原复制成功的大鼠关节炎模型,分成间接炙,青霉胺,病理组等三组动物。观察各组动物实验前后的关节指数变化,处死后各组踝关节以上关节的滑膜炎症,关节软骨及骨损害等病理变化。结果：关节指数,间接灸组与病理组有显著差异（P＜0．05）;关节炎,在Ⅲ,Ⅳ级中,间接灸组与病理组有显著差异（P＜0．05）。结论：间接炙与青霉胺对牛Ⅱ型胶原复制的大鼠关节炎模型有相同的作用,能控制滑膜炎症,减轻关节软骨和骨的损害。     

BMP对异种骨移植局部TNF-α和IL-6基因表达的调节

观察骨形态发生蛋白在异种骨移植局部对ＴＮＦ－α和ＩＬ－６ｍＲＮＡ表达的调节作用。方法将含有一定抗原性的牛松质骨载体与不同剂量的牛ＢＭＰ复合,植入小鼠股部肌袋,于术后５,１０和２０ｄ取材,制备石蜡切要用核酸分子原位杂交的方法,定性定位检测植骨局部ＴＮＦ－α和ＩＬ－６ｍＲＮＡ表达情况,通过图像分析,确定ＴＮＦ－α和ＩＬ－６ｍＲＮＡ表达强度,分析植骨局部不同计量ＢＭＰ对ＴＮＦ－α和ＩＬ－６ｍＲＮＡ表达强     

不同致伤条件撞击对兔钝性胸部创伤伤情影响的实验研究

探讨不同致伤参数对兔钝性胸部创伤后胸部筘器官伤情的影响。方法采用兔钝性胸部创伤型,设置不同的致伤参数,观察其对胸部各器 伤情影响及其对死亡率的影响。结果驱动压力越大,胸部各器官伤情越重;撞击面积越大,对胸壁和肺的损伤较重,但心脏伤情并不随之加重;收缩末期撞击对心脏的损伤较重,舒张末期撞击大血管系统的影响较大,死亡率与驱动压力、撞击面积成正;舒张末期撞击死亡率上升。结论致伤参数不同,对钝化胸部创伤后     

参附注射液对兔脊髓缺血损伤的保护作用

观察参附注射液对脊髓缺血性损伤的保护作用。方法１８只雄性新西兰大白兔,随机分为３组：Ａ组（ｎ＝６）即保护组,Ｂ组（ｎ＝６）即治疗组,Ｃ组（ｎ６）即对照组。Ａ组和Ｂ组分别于缺血前３０ｍｉｎ内或再灌注３０ｍｉｎ内持续恒速静脉输入参附注射液１０ｍＬ．ｋｇ＾－１;采用肾上主动脉阻断法造成脊髓缺血（２０ｍｉｎ）;术后观察神经功能变化并记录１,４,８,１２,２４和４８ｈ神经功能评分,４８ｈ处死动物后取脊髓（Ｌ     

一种新的融合表达载体的构建

利用Ｌ－ａｎｓＢ基因片段构建了融合表达载体ｐＥＣ,其中Ｌ－ａｎｓＢ基因中编码唯一酸水解位点的序列已通过定点突变消除,并在它的３’端引入了新的酸水解位点编码序列和方便外源基因插入的克隆位点ＢａｍＨＩ和ＨｉｎｄⅢ,外源基因可从克隆位点ＢａｍＨＩ和ＨｉｎｄⅢ处插入ｐＥＣ中,表达的融合蛋白用酸水解法加工后仅被切割成一大一小两个片段,不会导致产物混杂,ｈＧＲＦ对它进行验 结果显示ｈＧＲＦ基因不仅能按正确的读     

双氯酚酸钠米索前列醇片的抗炎作用研究

双氯酚酸钠米索前列醇片（ＤＳＭＴ）（２０,１０ｍｇ／ｋｇ）连续２ｄ灌胃给药可显著地抑制二甲苯所致鼠耳肿胀（Ｐ〈０．０１,Ｐ〈０．０５）;ＤＳＭＴ（１５,７．５ｍｇ／ｋｇ）灌胃给药均可非常显著抑制角叉菜胶至炎后３ｈ内大鼠足肿胀;ＤＳＭＴ高、低剂量组（１５,７．５ｍｇ／ｋｇ）灌胃给药７ｄdisplay structure