脊柱推拿时咔哒声响的测量技术分析与设计

目的 为研究脊住推拿时所发出咔哒声响，提供一套可采集、定位和分析的检测仪器。方法 在回顾咔哒声响测量技术现状的基础上，分析研究了实现声响测量的技术路线，设计了由时钟脉冲发生器、发送／接收接口、校准波发生器、复合传感器、计算机、测定分析软件以及输出部件等组成的声响测量系统。结果 经初步试验，在40cm的距离上测到了关节发出的咔哒声响，说明技术上基本可行。结论 本仪器可以作为脊柱推拿所致咔哒声响的采集、定位和分析系统使用。     

Acute peripheral arterial and graft occlusion: treatment with selective infusion of urokinase and lysyl plasminogen

Thirty-five patients hospitalized for recent angiographically documented arterial occlusion in the legs (27 femoropopliteal arteries and eight grafts) benefited from local fibrinolytic therapy delivered at the site of the occlusion with a 4- or 5-F catheter. This therapy combined a continuous urokinase (UK) infusion of 1,000 U/kg/hour and a lysyl plasminogen (LYS-PLG) infusion of 15 microkatals every 30 minutes. Angiographically confirmed lysis was obtained in 85% of the cases. Only 3% of the patients had major and 6% had minor groin hematomas. Only two patients had concentrations of fibrinogen as low as 100 mg/dl. Intravascular infusion of UK-LYS-PLG is as effective as streptokinase. Its excellent tolerance makes it a good alternative in the treatment of acute ischemia in the lower limbs.     

乙状结肠扭转40例的诊断与治疗

目的 探讨乙状结肠扭转的病因、诊断及治疗方法。方法 对1990年1月-2002年5月诊治的40例乙状结肠扭转的临床资料进行回顾性分析。结果 术前明确诊断31例、误诊为粘连性肠梗阻3例、绞窄性小肠梗阻2例。消化道穿孔弥漫性腹膜炎2例、腹痛待查2例。行非手术治疗8例,其中4例复发;手术治疗32例,其中单纯复位术11例,2例复发;复位加固定术8例,1例复发;复位加系膜折叠术6例;Hartmann术3例;乙状结肠切除一期吻合术4例。治愈38例,死亡2例。结论 本病的诊断主要依靠临床表现及腹部X线检查。治疗以手术为主,可根据病情及扭转情况选择适当的术式。     

头孢呋辛酯制备工艺的改进

以头孢呋辛钠为原料，从制备（R，S）-乙酸1-溴乙酯开始。经酯化反应的溶剂沉淀过程无须分离直接制得高纯度非晶型的头孢呋辛酯。本制备方法简便，适于工为化生产。     

葛根对小白鼠免疫功能的影响

从免疫学方面探讨中药葛根的强身健体作用。方法 :用 10 0 %葛根水浸出液定期给小鼠灌胃 ,然后检测小鼠抗体产生能力、淋巴细胞转化率、巨噬细胞吞噬的功能。结果 :用药组小白鼠以上免疫学指标均有显著增强作用 ,与对照组相比有非常显著性差异 (P <0 .0 1)。未见有副作用。结论 :口服葛根可明显增强机体免疫功能。     

In situ hybridization of prostate-specific antigen mRNA in human prostate.

Prostate-specific antigen (PSA) mRNA was detected by in situ hybridization utilizing a 428 base pair [35S]-labelled cDNA probe from the 3' noncoding region of the PSA gene. Thirty six fresh surgical specimens were collected from patients undergoing radical retropubic prostatectomy for carcinoma of the prostate. Quantitative analysis of the levels of PSA mRNA in both the benign and malignant tissues was performed using an IBAS 2000 Image Analysis System. The results of this study demonstrated that there is a significant decrease in the expression of PSA mRNA in the carcinoma tissue when compared to the benign epithelium. The average binding (number of silver grains/1 x 10(4) microns. 2) for 20 specimens of malignant epithelium was 475 +/- 161 and 586 +/- 140 for 16 specimens of benign epithelium (p less than 0.05). Eleven patients had both benign and malignant tissue from the same surgical specimen available for study. From these paired specimens, the PSA mRNA expression was also significantly reduced in the malignant epithelium when compared to the benign epithelium, 445 +/- 162 and 588 +/- 135 respectively (p less than 0.005). The PSA protein was detected using a monoclonal antibody to PSA with an immunohistochemical staining technique. The PSA protein expression paralleled the expression of the PSA mRNA in the majority of the tissue sections. Many of the tumor specimens showed a heterogeneous expression of PSA, whereas all of the benign epithelium had a uniform high level of PSA expression. In conclusion, PSA mRNA and protein are located only within the glandular epithelial tissue, the expression of PSA protein parallels that of the PSA mRNA, and both the PSA protein and PSA mRNA are significantly decreased in the malignant epithelium when compared to benign prostatic epithelium.     

伽玛刀治疗垂体大腺瘤临床分析(附30例报告)

目的 研究伽玛刀治疗垂体大腺瘤的效果及影响疗效的因素。方法 30例垂体大腺瘤病人用1．0T磁共振定位，Gamma-plan计划系统规划，等剂量曲线45％～60％(平均49．7％)，周边剂量12～35Gy(平均22.9Gy)，中心剂量24～70Gy(平均45．4Gy)．Leksell伽玛刀实施放射外科治疗。分析治疗后垂体大腺瘤病例，总结其疗效和影响疗效的因素。结果 随访12～60个月，平均28个月，肿瘤完全消失6例(20％)，肿瘤缩小19例(63．3％)，无变化5例(16．7％)；19／20(95．5％)功能性垂体腺瘤内分泌症状改善，临床症状好转，1例过早绝经(年龄36岁)；5／10(50％)无功能性垂体腺瘤体积缩小．症状改善。结论 伽玛刀对于垂体大腺瘤仍是一种有效的治疗方法，其疗神与腺瘤的举类型、体积、照射剂量、剂量体剂、MRI信号特征密切相姜。     

广东省结核病流行菌株研究

目的：从分子流行病学的角度探讨广东省结核分支杆菌菌株流行的分布。方法：构建广东省74株临床分离的以RFLP为基础的结核分支杆菌的IS6110 DNA指纹图谱技术，确诊结核分支杆菌菌株的流行分布。结果：24．3％（18／74）的结核菌株的IS6110 DNA指纹相似值在1－0．65之间，鉴定结果为它们均是“北京家族”结核分支杆菌菌株。结论：广东省结核菌株流行主要存在着遗传关系接近，在基因水平上相关程度较高的“北京家族”结核分支杆菌菌株，且该家族菌株正以一定的比例在我省流行。     

臀、会阴及其周围Ⅲ度烧伤的早期切痂治疗体会

目的　总结臀、会阴及其周围Ⅲ度烧伤的早期切痂治疗的经验。方法　对 3 2例臀、会阴Ⅲ度烧伤患者于伤后 3～ 7d内行切痂 ,嵌皮、大张皮片或皮瓣修复创面的资料进行回顾分析。结果　本组病例皮瓣、皮片大部分成活好 ,外观、功能恢复满意。结论　臀、会阴及周围Ⅲ度烧伤早期切痂治疗可缩短疗程 ,减少外观及功能障碍 ,近远期效果好