雪莲水溶性多糖提取工艺的研究

雪莲多糖是一种水溶性的功能性天然多糖,作者比较了料液比、温度、提取时间等因素对新疆雪莲组织培养物中雪莲多糖提取率的影响。结果表明,雪莲水溶性多糖的最佳提取工艺条件为料液比为1 g∶12 mL,温度85℃,时间2 h.粗多糖最大得率为7.08%。此粗多糖总糖质量分数为15.37%,从糖含量的角度,新疆雪莲组织培养物提取的粗多糖是野生雪莲提取的粗多糖的优良替代物品。     

再论肠内营养在消化道肿瘤早期应用的重要性

肠内营养（EN）是经胃肠道、用口服或管饲来提供代谢需要的营养基质及其他各种营养素的支持方式。肠黏膜因缺血、感染、损伤与营养不良而发生肠屏障功能障碍,从而引起肠源性感染,肠道细菌移位导致的肠源性感染成为外科领域中的一个重要研究课题。“只要肠道有营养功能时,就要采用肠内营养”的原则已越来越在临床得到体现。     

光纤药物溶出度监测仪在线监测米非司酮片的溶出度

目的：采用光纤化学传感技术,实时、在位监测米非司酮片的溶出度.方法：运用E1%/1cm值法监测米非司酮片的溶出全过程.结果：本法监测的溶出过程曲线与〈中国药典〉2005年版方法手动取样测定所拟合的曲线基本一致.结论：光纤药物溶出度监测仪可以准确测定固体药物的体外溶出度,真实地反映药物溶出的全过程.     

星状神经节阻滞治疗肩-手综合征的临床观察

目的:探讨星状神经节阻滞治疗肩-手综合征的临床疗效.方法:将52例肩-手综合征患者随机分为对照组(26例),实验组(26例),对照组进行常规治疗,实验组在对照组基础上联合星状神经节阻滞.评估2组治疗前后的视觉模拟评分(VAs)、关节活动度、肩臂水肿情况等.结果:2组治疗后VAS评分均较治疗前显著性下降(P<0.05),但实验组较对照组有显著性差异(P<0.05).实验组临床疗效优良率为88.5%,对照组为44.3%,2组有显著性差异(P<0.05).结论:星状神经节阻滞治疗肩-手综合征效果显著,并且操作简单,起效快.     

医用臭氧治疗腰椎间盘突出症临床观察

椎间盘突出症是临床常见病,多发病。现在随着医学技术的发展,微创治疗腰椎间盘突出症已成为一种趋势,为广大医生和患者所接受。经皮穿刺医用臭氧（O3）椎间盘内注射治疗椎间盘突出症是近几年来国内兴起的一项新技术,因其安全性高,不破坏损伤脊柱的结构,不易发生血管损伤,感染几率小等优点而备受关注。我们2006年1月-2007年1月用医用臭氧注射治疗椎间盘突出症患者112例,取得了满意的治疗效果,现报告如下。     

核苷类似物治疗早中期HBV-ACLF患者随机对照临床研究

目的 研究核苷类似物抗病毒治疗慢加急性乙型肝炎肝衰竭（HBV-ACLF）早中期患者的疗效和安全性.方法 采用前瞻性随机开放平行对照临床实验设计,设基础治疗组、拉米夫定＋基础治疗组及恩替卡韦＋基础治疗组三组进行对照观察研究.结果 治疗1个月时三组生存率相近,但拉米夫定、恩替卡韦治疗组临床好转率显著高于基础治疗组;治疗随访6个月拉米夫定及恩替卡韦累积生存率分别为65.8%、60.1%,显著高于基础治疗组42%（P〈0.05）.治疗前HBV DNA〉107IU/L患者接受抗病毒治疗组累积生存率高于基础治疗组,治疗前MELD评分〉30患者累积生存率低于MELD评分≤30患者,但MELD评分〉30患者抗病毒治疗的疗效反应性更好.结论 核苷类似物恩替卡韦及拉米夫定治疗HBV-ACLF早中期患者可以显著提高治疗1个月的好转率,降低3个月、6个月的病死率,提高患者生存率.     

HBV基因型与HBV感染慢性化、重症化的关系

Objective To explore the association between HBV genotype and chronic/severe liver disease with HBV infection in Chinese patients.Methods Serum samples were collected from 2922 patients with HBV infection.HBV genotyping was performed with type-specific primers polymerase chain reaction,and the virological and biochemical markers were detected,which differences in the genotypes between various clinical types of HBV infection and liver function and virological markers between various HBV genotyping were analyzed.Results The genotype B,C,BC combinations,D of 2922 patients with HBV infection accounted for 15.9%,83.5%,0.41%,0.21% respectively.The ratio of genotype B in acute hepatitis group was higher(P=0.003),which the ratio of genotype C in the cirrhosis group and the hepatocellular carcinoma group was higher(P=0.000,0.000).The difference in ratio of genotype C was not statistically significant between acute-on-chronic liver failure group and chronic hepatitis group.HBeAg-positive rate,viral load and liver function markers of B,C genotype group in acute hepatitis group and chronic hepatitis group were not significant different.HBeAg-positive rates of genotype C in acute-on-chronic liver failure group,cirrhosis group,hepatoeellular carcinoma group were higher than that of genotype B(P=0.000,0.024,0.003).Viral load of genotype C in hepatocellular carcinoma group was higher than that of genotype B(P=0.025).Cholinesterase levels of genotype C in the acute-on-chronic liver failure group and the hepatocellular carcinoma group was lower than that of genotype B(P=0.0004、0.02).Conclusion There were HBV genotype B,C,B/C combinations and D in Chinese patients with HBV infection,with genotype B and C being the major ones.Compared with HBV genotype B,genotype C in Chinese patients with HBV infection was more likely to chronic infection,evolved to cirrhosis and hepatocellular carcinoma, but genotype difference was not observed in occurrence of acute-on-chronic liver failure.Genotype was not significant effect in acute and chronic hepatitis B,but HBeAg-positive rate/viral load was higher and liver damage was more severe in severe and end-stage genotype C HBV infection patients.     

手法治疗实验性膝骨关节炎扫描电镜研究

观察手法防治兔膝骨关节炎扫描电镜下超微结构的变化，评价手法治疗的效果。方法：采用结扎股静脉，臂下静脉方法造成免骨内高压膝关节骨关节炎模型，术后第二天进行手法治疗，每日３次。结果实验组骨关节炎改变较对照组明显减轻。结论手法确能有效地防治骨关节炎。     

中药对去势兔骨代谢影响的骨计量学研究

运用骨计量学观察了中药（骨松Ⅱ号）对兔切除卵巢诱导的骨质疏松症实验模型的影响，并与尼尔雌醇进行对照。结果显示：骨松Ⅱ号与尼尔雌醇组在ＢＶ／ＴＶ、ＯＶ／ＢＶ、ＯＢＩ、ｄＬｓ／ＢＳ和ＢＦＲ（Ｔ）等参数方面明显高于造模组（ＯＶＸ）和／或正常对照组（ＳＨＡＭ），骨松Ⅱ号组ＯＶ／ＢＶ、ＯＢ％以及ＯＢＩ等参数较尼尔雌醇组明显升高。提示：骨松Ⅱ号能增加成骨细胞数量，以产生较多骨质，使骨质疏松症模型兔的骨代谢恢复正平衡，达到防治骨质疏松症的目的；其作用一定程度上优于尼尔雌醇。