术中剩余皮肤组织匀浆在网状皮移植中的应用

烧伤临床上进行皮肤移植手术时,因为术者对创面大小估计的误差、取皮质量、移植过程中皮片裁剪等因素,创面被充分覆盖后常有剩余供皮.为了充分利用这部分皮肤,我们将其制作为混悬匀浆,与网状皮肤混合移植于创面,观察其疗效.     

重组沙门菌表达结核分枝杆菌ESAT-6及其特异性免疫应答分析

Objective To determine the immune responses induced by recombinant Salmonella ty-phimurium expressing the secreting antigen ESAT-6 of Mycobacterium tuberculosis. Methods ESAT-6 cod-ing gene was cloned and identified by PCR and sequencing. Prokaryotic expression plasmid pYA33-esat car-rying the ESAT-6 coding sequence was constructed firstly and electro-transformed into an attenuated strain X4550 of Salmonella typhimurium, the recombinant bacteria was named as X4550(33-esat). C57BL/6 mice were immunized intranasally (I. N) with 108 CFU recombinant bacteria at day 0 and 18. Cells from spleen, lung, mesenteric lymph node (MLN) and Peyer's patch (PP) were collected from mice after second immu-nization, and the specific IFN-γ-secreting cells and IL-4-secreting cells were detected by ELISPOT assay u-sing ESAT-6 peptide as stimulus. Furthermore, CTL effects were in vivo evaluated by CFSE assay. Results The results showed that cellular immune responses specific for ESAT-6 could be detected by ELISPOT assay. In lung and PP cells, immune responses against ESAT-6 were biased toward Th1 type, the frequency of IFN-γ-secreting cells was much higher than that of IL-4-secreting cells. In splenocytes and MLN cells, the anti-gen specific immune responses acted as Thl and Th2 balance, the frequency of IFN-γ-secreting cells was close to that of IL-4-secreting cells. CFSE assay indicated that recombinant bacteria could induce the high level of CTL effects specific for ESAT-6 peptide. Conclusion These results suggested that recombinant Sal-monella typhimurium X4550(33-esat) not only can induce cellular immune responses, but also can elicit specific CTL responses after I. N immunization. It also provided the useful information for the control of infec-tious disease of tuberculosis.     

脑源性多器官功能衰竭

Ｎｅｕｒｏｇｅｎｉｃｍｕｌｔｉｐｌｅｏｒｇａｎｆａｉｌｕｒｅ脑源性多器官功能衰竭是由于脑的急性功能损害后所引起的脑以外２个或２个以上的器官功能衰竭［１］。本病发病机制复杂,死亡率高,但经过治疗在脑功能好转后,受损害的器官也有部分能好转。引起脑源性器官...