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991.
This study investigates the effect of various dietary saturated fatty acid (SFA) profiles on plasma lipid parameters and tissue
fatty acid composition in rats. The experiment was designed to monitor polyunsaturated fatty acids (PUFA) levels, while examining
different amounts and types of SFA. Four isocaloric diets were prepared, containing 10–11 mol% of fatty acids (FA) as linoleic
acid (LNA) and 2.5 mol% as α-linolenic acid (ALA), leading to an identical and well-balanced LNA/ALA ratio. The initial rapeseed
oil/corn oil mixture providing ALA and LNA was enriched with olive oil to prepare the olive oil diet. The butterfat diet was
supplemented with butterfat, containing short-chain SFA (C4:0–C10:0, 17 mol% of FA), lauric acid (C12:0, 3.2 mol%), myristic
acid (C14:0, 10.5 mol%) and palmitic acid (C16:0, 14.5 mol%). The saturates diet was supplemented with trilaurin, trimyristin
and tripalmitin to obtain the same level of lauric, myristic and palmitic acids as the butterfat diet, without the short-chain
SFA. The trimyristin diet was enriched with trimyristin only. The results showed that the butterfat diet contributed to specific
effects, compared to the olive oil diet and the saturates and trimyristin diets: a decrease in plasma total, LDL- and HDL-cholesterol,
higher tissue storage of ALA and LNA, and a higher level of (n-3) highly unsaturated fatty acids in some tissues. This study
supports the hypothesis that in diets with identical well-balanced LNA/ALA ratios, short chain SFA may decrease circulating
cholesterol and increase tissue polyunsaturated fatty acid content in the rat. 相似文献
992.
A study of the electric field and temperature dependence of the breakdown and quasi-breakdown phenomena is presented for 3.5 nm ultra-thin SiO2 gate oxides. Using à methodology based on the competing mecanism concept between breakdown and quasi-breakdown processes, quasi-breakdown activation energy as well as acceleration factor are determined. It is demonstrated on these 3.5nm gate oxides that the quasi-breakdown temperature activation energy is almost temperature independent on the contrary to the breakdown one. Moreover, it has been shown that the temperature dependence of the breakdown acceleration factor and the electric field dependence of the temperature activation energy cannot be explained by a pure “E” and “1/E” models, but can be interpreted by the “E” model if at least two types of molecular defect states are considered. 相似文献
993.
Identification of the nonlinear excitation force acting on a bowed string using the dynamical responses at remote locations 总被引:1,自引:0,他引:1
For achieving realistic numerical simulations of bowed string instruments, based on physical modeling, a good understanding of the actual friction interaction phenomena is of great importance. Most work published in the field including our own has assumed that bow/string frictional forces behave according to the classical Coulomb stick-slip model, with an empirical velocity-dependent sliding friction coefficient. Indeed, the basic self-excited string motions (such as the Helmholtz regime) are well captured using such friction model. However, recent work has shown that the tribological behavior of the bow/string rosin interface is rather complex, therefore the basic velocity-dependent Coulomb model may be an over-simplistic representation of the friction force. More specifically, it was suggested that a more accurate model of the interaction force can be achieved by coupling the system dynamical equations with a thermal model which encapsulates the complex interface phenomena. In spite of the interesting work performed by Askenfelt [32], a direct measurement of the actual dynamical friction forces without disturbing the string motion is quite difficult. Therefore, in this work we develop a modal-based identification technique making use of inverse methods and optimization techniques, which enables the identification of the interface force, as well as the string self-excited motion, from the dynamical reactions measured at the string end supports. The method gives convincing results using simulated data originated from nonlinear computations of a bowed string. Furthermore, in cases where the force identifications are very sensitive to errors in the transfer function modal parameters, we suggest a method to improve the modal frequencies used for the identifications. Preliminary experimental results obtained using a basic bowing device, by which the string is excited with the stick of the bow, are then presented. Our identifications, from the two dynamical string reactions, are consistent as attested by the comparison of the two available versions of the string dynamical motion and of the friction force. Furthermore, the method seems adequate to investigate the interface force for the bowed string. 相似文献
994.
本实验探讨影响GUMS以及LC/MS/MS检测灵敏度的影响因素.对GUMS法的固相萃取(SPE)和衍生化条件进行优化,测试了SPE、加热时间、温度、溶液凡式归匕等因素.同时测试这些条件对LUMC/MC法的灵敏度.结果表明,碱基化为影响GUMS法灵敏度的主要因素,能提高灵敏度,对衍生化条件也有影响.通过对样本进行固相萃取,速日处理,GUMS法的灵敏度为0.5 ng/mL,LC/MC/MC法的为0.25 ng/mL.采用这种更灵敏的方法,尿液中莱克多巴胺的测试窗口期可由2d提高到6d.经过优化的GUMS法及LC/MS/MS方法灵敏度更高,适合猪尿中莱克多巴胺的检测. 相似文献
995.
A sensitive method for the quantification of acrolein and other volatile carbonyls in ambient air 总被引:1,自引:0,他引:1
Acrolein, an unsaturated aldehyde found in both indoor and outdoor air, is considered one of the greatest noncancer health risks of all organic air pollutants. Current methods for determining acrolein often employ sorbent-filled cartridges containing a carbonyl derivatizing agent (e.g., dinitrophenylhydrazine). These methods are of limited use for unsaturated compounds due to the formation of unstable derivatives, coelution of similar compounds, long sample collection times, and ozone interferences that result in poor sensitivity, selectivity, and reproducibility. The goal of this research was to develop an analytical method for determining ppt concentrations of acrolein and other carbonyls in air with short sampling times (10 min). The method uses a mist chamber to collect carbonyls by forming water-soluble carbonyl-bisulfite adducts. The carbonyls are then liberated from the bisulfite, derivatized, and quantified by gas chromatography/electron capture negative ionization mass spectrometry. The method was applied to determine atmospheric acrolein concentrations at three sites in northern California reflecting hemispheric background concentrations, biogenic-dominated regions, and urban environments. The resulting acrolein concentrations were 0.056, 0.089, and 0.29 microg/m3, respectively, which are all above the EPA Reference Concentration of 0.02 microg/m3. The minimum detection limit of 0.012 microg/m3 is below that of other published methods. Methacrolein, methyl vinyl ketone, crotonaldehyde, glyoxal, methyl glyoxal, and benzaldehyde were also quantified. 相似文献
996.
Cationic and anionic nanoparticles selectively target peptides with low and high isoelectric points, respectively. Additionally, their high surface area-to-volume ratios make these nanoparticles (approximately 2-nm core diameter) very efficient extraction and concentration agents. Upon extraction, the peptide-bound nanoparticles can be analyzed by MALDI-MS to provide highly sensitive detection of the targeted peptides. We demonstrate that MALDI-MS can detect peptide concentrations as low as 500 pM from 250-microL solutions using these nanoparticle scaffolds as extraction and concentration agents. 相似文献
997.
Frequency changes induced by bias or temperature modulation of injection diode lasers can provide an economical and effective method of applying phase-stepping interferometry to optical metrology. However, the intrinsic frequency instability of these devices limits their use in gauge block interferometry where precise and repeatable phase steps must be maintained simultaneously on two discontinuous surfaces and over relatively long path lengths. We demonstrate a method using a visible injection diode laser, the frequency of which is locked by using a Fabry-Perot interferometer. Small changes to the length of the Fabry-Perot interferometer shift the frequency of the laser producing proportional and repeatable phase steps to the gauge block interferogram. This method has been successfully implemented with a Fizeau-type gauge block interferometer with a phase measurement resolution of 0.005 lambda. The phase data are then processed to map the surface form of gauge blocks up to 100 mm in length and to objectively assess surface shape parameters. 相似文献
998.
We analyze the effect of spurious diffraction orders when generating functional multifoci patterns produced by illuminating a phase-only hologram with a single Gaussian beam. Using a practical device for encoding a hologram generates an undesirable zero order and high-diffraction orders at the Fourier plane. This translates to the fact that a significant fraction of the incident light does not necessarily convert to functional multifoci patterns. In most applications, the zero order can be avoided by generating foci patterns shifted off the optical axis, which further increases the amount of light distributed to spurious high-diffraction orders owing to the reduction of light directed to the desired foci pattern. We analyze the amount of light dispersed to spurious orders and show that these unwanted orders can be a major limiting factor for most applications based on arbitrary multifoci patterns. 相似文献
999.
The disaccharide trehalose is accumulated by microorganisms, such as yeasts, and multicellular organisms, such as tardigrades, when conditions of extreme drought occur. In this way these organisms can withstand dehydration through the formation of an intracellular carbohydrate glass, which, with its high viscosity and hydrogen-bonding interactions, stabilizes and protects the integrity of complex biological structures and molecules. This property of trehalose can also be harnessed in the stabilization of liposomes, proteins and in the preservation of red blood cells, but the underlying mechanism of bioprotection is not yet fully understood. Here we use positron annihilation lifetime spectroscopy to probe the free volume of trehalose matrices; specifically, we develop a molecular picture of the organization and mobility of water in both amorphous and crystalline states. Whereas in amorphous matrices, water increases the average intermolecular hole size, in the crystalline dihydrate it is organized as a confined one-dimensional fluid in channels of fixed diameter that allow activated diffusion of water in and out of the crystallites. We present direct real-time evidence of water molecules unloading reversibly from these channels, thereby acting as both a sink and a source of water in low-moisture systems. We postulate that this behaviour may provide the overall stability required to keep organisms viable through dehydration conditions. 相似文献
1000.
Dixit SK Goicochea NL Daniel MC Murali A Bronstein L De M Stein B Rotello VM Kao CC Dragnea B 《Nano letters》2006,6(9):1993-1999
Incorporation of CdSe/ZnS semiconductor quantum dots (QDs) into viral particles provides a new paradigm for the design of intracellular microscopic probes and vectors. Several strategies for the incorporation of QDs into viral capsids were explored; those functionalized with poly(ethylene glycol) (PEG) can be self-assembled into viral particles with minimal release of photoreaction products and enhanced stability against prolonged irradiation. 相似文献