Absence of human herpesvirus 8 and Epstein-Barr virus genome sequences in cutaneous epithelial neoplasms arising in immunosuppressed organ-transplant patients

Recent studies have implicated herpesvirus 8 and Epstein-Barr virus in the development of cutaneous malignancies in immunosuppressed patients. In order to examine the strength of this association, we examined 37 malignant, pre-malignant and benign cutaneous epithelial neoplasms removed from immunosuppressed organ recipients for the presence of human herpesvirus 8 and Epstein-Barr viral genome sequences using polymerase chain reaction (PCR) and in situ hybridization. We examined 2 actinic keratoses, 1 benign keratosis, 11 invasive squamous cell carcinomas, 17 squamous cell carcinomas in situ and 6 basal cell carcinomas. We also examined 4 basal cell carcinomas, 1 invasive squamous cell carcinoma and 3 squamous cell carcinomas in immunocompetent hosts. In contrast to findings reported by other investigators, we were unable to detect viral genome sequences in any of the biopsies examined. Our findings suggest that human herpesvirus 8 and Epstein-Barr virus likely do not play an etiologic role in cutaneous epithelial oncogenesis in immunocompromised patients.     

An Indexing Method for Answering Queries on Moving Objects

We consider the problem of indexing a set of objects moving in d-dimensional spaces along linear trajectories. A simple external-memory indexing scheme is proposed to efficiently answer general range queries. The following are examples of the queries that can be answered by the proposed method: report all moving objects that will (i) pass between two given points within a specified time interval; (ii) become within a given distance from some or all of a given set of other moving objects. Our scheme is based on mapping the objects to a dual space, where queries about moving objects are transformed into polyhedral queries concerning their speeds and initial locations. We then present a simple method for answering such polyhedral queries, based on partitioning the space into disjoint regions and using a B+-tree to index the points in each region. By appropriately selecting the boundaries of each region, we guarantee an average search time that matches a known lower bound for the problem. Specifically, for a fixed d, if the coordinates of a given set of N points are statistically independent, the proposed technique answers polyhedral queries, on the average, in O((N/B)^1−1/d⋅(log _B N)^1/d+K/B) I/O's using O(N/B) space, where B is the block size, and K is the number of reported points. Our approach is novel in that, while it provides a theoretical upper bound on the average query time, it avoids the use of complicated data structures, making it an effective candidate for practical applications. The proposed index is also dynamic in the sense that it allows object insertion and deletion in an amortized update cost of log _B(N) I/O's. Experimental results are presented to show the superiority of the proposed index over other methods based on R-trees. recommend Ahmed Elmagarmid     

On selecting a low-order model using the dominant mode concept

A technique for selecting a low-order system to approximate a high-order model has been suggested by Davison [2]. A critical component in this technique is the criterion used to select the most appropriate order and modes for the low-order approximation. Criteria have been discussed and analyzed by Mahapatra [5], [6], Rao, Lamba, and Rao [7], and Elrazaz and Sinha [3]. In this note we overcome deficiencies in the criteria that have been proposed and we introduce a new criterion which is rigorously justified. The criterion we suggest is also applicable when eigenvalues of the system are nonreal.     

Changes in fatty acid and essential oil composition of sage (Salvia officinalis L.) leaves under NaCl stress

Fatty acids and essential oils from hydroponically cultivated Salvia officinalis leaves were analyzed by GC and GC–MS. Four different levels of NaCl (25, 50, 75 and 100 mM) were applied. The first results showed that salt treatment reduced significantly the plant growth by 61% and the total fatty acids (TFA) content by 32% at 100 mM NaCl. Alpha-linolenic, gadoleic, palmitic and oleic acids were the major fatty acids. Moreover, the polyunsaturated fatty acids decreased, while the monounsaturated ones increased with respect to increasing salinity. Regarding the essential oil composition, the main compounds were α-and β-thujone, 1,8-cineole, camphor, α-humulene, viridiflorol and manool at all salt treatments. The yield had a maximum increase at 75 mM NaCl. Hence, sage can be considered as moderately salt sensitive.     

微波及辐射真空干燥过程中的干燥动力学及能量消耗

The general objective of this work is to analyze energy input in a vacuum process with the incorporation of microwave heating. Thus, necessary criteria for designing an efficient freeze-drying operation are considered through the analysis of strategies based on the combination of different intensities of raxiiant and microwave heating.The other aim of this research topic is to study the kinetics of drying in relation to mass transfer parameters.Five freeze-drying strategies using both heating systems were used. Consequently, energy input could be related to diffusivity coefficients, temperature and pressure profiles during dehydration of the product and analyzed in comparison to a conventional freeze-drying process.     

Lamin-Related Congenital Muscular Dystrophy Alters Mechanical Signaling and Skeletal Muscle Growth

Laminopathies are a clinically heterogeneous group of disorders caused by mutations in the LMNA gene, which encodes the nuclear envelope proteins lamins A and C. The most frequent diseases associated with LMNA mutations are characterized by skeletal and cardiac involvement, and include autosomal dominant Emery–Dreifuss muscular dystrophy (EDMD), limb-girdle muscular dystrophy type 1B, and LMNA-related congenital muscular dystrophy (LMNA-CMD). Although the exact pathophysiological mechanisms responsible for LMNA-CMD are not yet understood, severe contracture and muscle atrophy suggest that mutations may impair skeletal muscle growth. Using human muscle stem cells (MuSCs) carrying LMNA-CMD mutations, we observe impaired myogenic fusion with disorganized cadherin/β catenin adhesion complexes. We show that skeletal muscle from Lmna-CMD mice is unable to hypertrophy in response to functional overload, due to defective fusion of activated MuSCs, defective protein synthesis and defective remodeling of the neuromuscular junction. Moreover, stretched myotubes and overloaded muscle fibers with LMNA-CMD mutations display aberrant mechanical regulation of the yes-associated protein (YAP). We also observe defects in MuSC activation and YAP signaling in muscle biopsies from LMNA-CMD patients. These phenotypes are not recapitulated in closely related but less severe EDMD models. In conclusion, combining studies in vitro, in vivo, and patient samples, we find that LMNA-CMD mutations interfere with mechanosignaling pathways in skeletal muscle, implicating A-type lamins in the regulation of skeletal muscle growth.     

New approach to mimic rheological actual shear rate under wall slip condition

Engineering with Computers - The presence of wall slip in concentrated suspensions affect the rheological measurements such as shear stress, shear rate, and viscosity. The measured shear rate will...