Level clipped high-order OFDM

The asymptotic behavior of level clipped orthogonal frequency-division multiplexing when its order N approaches infinity is analyzed. It is shown that if the clipping level approaches infinity faster than √lnN, then a zero bit-error rate penalty and arbitrarily large peak to-average power ratio (PAPR) gain are asymptotically obtained. Moreover, the expression for the probability of clipping is found, and using it, the upper bound of the probability of error is derived. This upper bound can be used to analyze the tradeoff between PAPR and probability of error     

Low-Frequency Ultrasound Effects on Intracellular Barriers in Nonviral Gene Delivery Processes

Gene therapy, the expression in cells of genetic material with therapeutic activity, has emerged as a promising approach for the treatment or prevention of human diseases. At the present time, major somatic gene-transfer approaches employ either viral or nonviral vectors. Nonviral vectors are less efficient at introducing and maintaining foreign gene expression, but have the profound advantage of being nonpathogenic and nonimmunogenic. In this study, we aimed to develop an efficient nonviral gene delivery system in which low-frequency ultrasound (LFUS) was applied to enhance gene expression of polyplexes formed with poly(2-dimethylaminoethyl methacrylate) and plasmid encoding for green fluorescent protein. Ultrasound (US), and in particular LFUS, can cause temporary membrane permeabilization and thereby enhance drug and gene entrance into viable cells. We evaluated possible additional favorable effects of LFUS on the polyplex transfection process, such as overcoming intracellular barriers. We found that pDMAEMA protected the plasmid DNA from ultrasonic degradation. Atomic force microscopy analysis also confirmed that the LFUS did not change the polyplexes’ morphology. We also attained an insight into the structure of polyplexes during LFUS exposure and found that LFUS induced a temporary partial detachment between the polymer chains and the plasmid. In addition, LFUS application on ovarian carcinoma cells transfected with the polyplexes induced a 27% enhancement in transfection efficiency. Based on these results, we propose that LFUS enhances the decomplexation of the polyplexes, and therefore, can be used to optimize transfection efficiency.     

Treating phobic children: Effects of EMDR versus exposure.

This study examined the efficacy of eye movement desensitization and reprocessing (EMDR) and exposure in the treatment of a specific phobia. Twenty-six spider phobic children were treated during 2 treatment phases. During the first phase, which lasted 2.5 hr, children were randomly assigned to either (a) an EMDR group (n?=?9), (b) an exposure in vivo group (n?=?9), or (c) a computerized exposure (control) group (n?=?8). During the 2nd phase, all groups received a 1.5-hr session of exposure in vivo. Therapy outcome measures (i.e., self-reported fear and behavioral avoidance) were obtained before treatment, after Treatment Phase 1, and after Treatment Phase 2. Results showed that the 2.5-hr exposure in vivo session produced significant improvement on all outcome measures. In contrast, EMDR yielded a significant improvement on only self-reported spider fear. Computerized exposure produced nonsignificant improvement. Furthermore, no evidence was found to suggest that EMDR potentiates the efficacy of a subsequent exposure in vivo treatment. Exposure in vivo remains the treatment of choice for childhood spider phobia. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Changes in chemical constituents during the maturation and ripening of two commercially important pomegranate accessions

Pomegranate juice is well known for its health beneficial compounds. This study was undertaken to investigate changes in the major chemical composition in arils and peels during fruit maturation in two Israeli commercial accessions, ‘Wonderful’ and ‘Rosh-Hapered.’ In both accessions, the levels of total phenolic, antioxidant activity and hydrolysable tannins were reduced in the peels during maturation, while the anthocyanin level increased. The results show that the sugar content in the aril juice increased in both accessions while the levels of acidity and of citric acid decreased. However, these two accessions differed in other parameters in the aril juice, i.e., while the antioxidant and total phenolic contents significantly decreased in ‘Rosh-Hapered’, these changes were not observed in ‘Wonderful’. The anthocyanin level, however, increased in ‘Wonderful’ but did not change in ‘Rosh-Hapered.’ This knowledge could help establish the optimum harvest date ensuring the maximum nutritional properties of pomegranates.     

Gaucher Disease Diagnosis Using Lyso-Gb1 on Dry Blood Spot Samples: Time to Change the Paradigm?

For years, the gold standard for diagnosing Gaucher disease (GD) has been detecting reduced β-glucocerebrosidase (GCase) activity in peripheral blood cells combined with GBA1 mutation analysis. The use of dried blood spot (DBS) specimens offers many advantages, including easy collection, the need for a small amount of blood, and simpler transportation. However, DBS has limitations for measuring GCase activity. In this paper, we recount our cross-sectional study and publish seven years of experience using DBS samples and levels of the deacylated form of glucocerebroside, glucosylsphingosine (lyso-Gb1), for GD diagnosis. Of 444 screened subjects, 99 (22.3%) were diagnosed with GD at a median (range) age of 21 (1–78) years. Lyso-Gb levels for genetically confirmed GD patients vs. subjects negative to GD diagnosis were 252 (9–1340) ng/mL and 5.4 (1.5–16) ng/mL, respectively. Patients diagnosed with GD1 and mild GBA1 variants had lower median (range) lyso-Gb1, 194 (9–1050), compared to GD1 and severe GBA1 variants, 447 (38–1340) ng/mL, and neuronopathic GD, 325 (116–1270) ng/mL (p = 0.001). Subjects with heterozygous GBA1 variants (carrier) had higher lyso-Gb1 levels, 5.8 (2.5–15.3) ng/mL, compared to wild-type GBA1, 4.9 (1.5–16), ng/mL (p = 0.001). Lyso-Gb1 levels, median (range), were 5 (2.7–10.7) in heterozygous GBA1 carriers with Parkinson’s disease (PD), similar to lyso-Gb1 levels in subjects without PD. We call for a paradigm change for the diagnosis of GD based on lyso-Gb1 measurements and confirmatory GBA1 mutation analyses in DBS. Lyso-Gb1 levels could not be used to differentiate between heterozygous GBA1 carriers and wild type.     

Do Adolescent Exposure to Cannabinoids and Early Adverse Experience Interact to Increase the Risk of Psychiatric Disorders: Evidence from Rodent Models

There have been growing concerns about the protracted effects of cannabis use in adolescents on emotion and cognition outcomes, motivated by evidence of growing cannabis use in adolescents, evidence linking cannabis use to various psychiatric disorders, and the increasingly perceived notion that cannabis is harmless. At the same time, studies suggest that cannabinoids may have therapeutic potential against the impacts of stress on the brain and behavior, and that young people sometimes use cannabinoids to alleviate feelings of depression and anxiety (i.e., “self-medication”). Exposure to early adverse life events may predispose individuals to developing psychopathology in adulthood, leading researchers to study the causality between early life factors and cognitive and emotional outcomes in rodent models and to probe the underlying mechanisms. In this review, we aim to better understand the long-term effects of cannabinoids administered in sensitive developmental periods (mainly adolescence) in rodent models of early life stress. We suggest that the effects of cannabinoids on emotional and cognitive function may vary between different sensitive developmental periods. This could potentially affect decisions regarding the use of cannabinoids in clinical settings during the early stages of development and could raise questions regarding educating the public as to potential risks associated with cannabis use.     

Understanding Declare models: strategies,pitfalls, empirical results

Declarative approaches to business process modeling are regarded as well suited for highly volatile environments, as they enable a high degree of flexibility. However, problems in understanding and maintaining declarative process models often impede their adoption. Likewise, little research has been conducted into the understanding of declarative process models. This paper takes a first step toward addressing this fundamental question and reports on an empirical investigation consisting of an exploratory study and a follow-up study focusing on the system analysts’ sense-making of declarative process models that are specified in Declare. For this purpose, we distributed real-world Declare models to the participating subjects and asked them to describe the illustrated process and to perform a series of sense-making tasks. The results of our studies indicate that two main strategies for reading Declare models exist: either considering the execution order of the activities in the process model, or orienting by the layout of the process model. In addition, the results indicate that single constraints can be handled well by most subjects, while combinations of constraints pose significant challenges. Moreover, the study revealed that aspects that are similar in both imperative and declarative process modeling languages at a graphical level, while having different semantics, cause considerable troubles. This research not only helps guiding the future development of tools for supporting system analysts, but also gives advice on the design of declarative process modeling notations and points out typical pitfalls to teachers and educators of future systems analysts.