Laboratory Measurements and a Theoretical Analysis of the TCT Fading Channel Radio System

This paper documents the laboratory and theoretical performance of a pilot-aided digital radio system. The technique considered transmits a midband pilot tone to improve the receiver data detection performance in a multipath fading channel and is referred to as the tone calibrated technique (TCT). We report on the performance of a 860 MHz prototype system carrying 2.4 kbit/s data under Rician fading conditions. Both experimental and analytical results show that the error floor experienced with nonpilot-aided transmission methods is effectively removed by the TCT scheme, resulting in significant performance gains at high signal-to-noise ratio (SNR) values. The paper also examines the TCT system performance under typical operating conditions and presents a new analysis of the TCT theoretical error probability.     

Evolutionary link between glycogen phosphorylase and a DNA modifying enzyme

We report here an unexpected similarity in three-dimensional structure between glucosyltransferases involved in very different biochemical pathways, with interesting evolutionary and functional implications. One is the DNA modifying enzyme beta-glucosyltransferase from bacteriophage T4, alias UDP-glucose:5-hydroxymethyl-cytosine beta-glucosyltransferase. The other is the metabolic enzyme glycogen phosphorylase, alias 1.4-alpha-D-glucan:orthophosphate alpha-glucosyltransferase. Structural alignment revealed that the entire structure of beta-glucosyltransferase is topographically equivalent to the catalytic core of the much larger glycogen phosphorylase. The match includes two domains in similar relative orientation and connecting helices, with a positional root-mean-square deviation of only 3.4 A for 256 C alpha atoms. An interdomain rotation seen in the R- to T-state transition of glycogen phosphorylase is similar to that observed in beta-glucosyltransferase on substrate binding. Although not a single functional residue is identical, there are striking similarities in the spatial arrangement and in the chemical nature of the substrates. The functional analogies are (beta-glucosyltransferase-glycogen phosphorylase): ribose ring of UDP-pyridoxal ring of pyridoxal phosphate co-enzyme; phosphates of UDP-phosphate of co-enzyme and reactive orthophosphate; glucose unit transferred to DNA-terminal glucose unit extracted from glycogen. We anticipate the discovery of additional structurally conserved members of the emerging glucosyltransferase superfamily derived from a common ancient evolutionary ancestor of the two enzymes.     

Null cell adenomas and oncocytomas of the pituitary gland

This review highlights the most interesting features of null cell adenomas and oncocytomas of the pituitary gland. Recently, application of sensitive methods have documented a very low amount of active hormone production in these clinically nonfunctioning tumours. However, further studies are needed to clarify the histogenesis of null cell adenomas and oncocytomas.     

Idiotype immunization combined with granulocyte-macrophage colony-stimulating factor in myeloma patients induced type I, major histocompatibility complex-restricted, CD8- and CD4-specific T-cell responses

Idiotypic structures expressed on the myeloma Ig protein might be regarded as a tumor-specific antigen. Five patients with IgG myeloma were immunized with the purified serum M-component by repeated intradermal injections together with soluble granulocyte-macrophage colony-stimulating factor (GM-CSF). All patients developed an idiotype (Id)-specific T-cell immunity, defined as blood T cells predominantly secreting interferon-gamma (IFN-gamma) and interleukin-2 (IL-2) (type I cells). Id-specific DNA synthesis was induced in one patient. Delayed-type hypersensitivity against the Id was not evoked. The specific IFN-gamma/IL-2 T-cell response was inhibited (46% to 100%) by a major histocompatibility complex (MHC) class I monoclonal antibody (MoAb) in all five patients. A 5% to 37% inhibition by an MHC class II MoAb was seen in four patients. CD4+ as well as CD8+ T cells enriched by magnetic microbeads contained Id-specific cells. The T cells recognized peptides corresponding to the complementarity-determining regions 1, 2, and 3 of the heavy chain of the Id. There was a transient rise of B cells producing IgM anti-idiotypic antibodies in all patients. The results indicate that immunization of myeloma patients using the autologous M-component and soluble GM-CSF may evoke an Id-specific predominantly MHC class I-restricted type I T-cell response.     

Combining concatenation and formant synthesis for improved intelligibility and naturalness in text-to-speech systems

A general method which combines formant synthesis by rule and time-domain concatenation is proposed. This method utilizes the advantages of both techniques by maintaining naturalness while minimizing difficulties such as prosodic modification and spectral discontinuities at the point of concatenation. An integrated sampled natural glottal source (Matsui et al., 1991) and sampled voiceless consonants were incorporated into a real-time text-to-speech formant synthesizer. In special cases, voicing amplitude envelopes and formant transitions dirived from natural speech were also utilized. Several listening tests were performed to evaluate these methods. We obtained a significant overall improvement in intelligibility over our previous formant synthesizer. Such improvements in intelligibility were previously obtained with a Japanese text-to-speech system using a related hybrid system (Kamai and Matsui, 1993), indicating the applicability of this method for multi-lingual synthesis. The results of subjective analyses showed that these methods can alo improve naturalness and listenability factors.     

The Role of Institutional Review Boards in Facilitating Research, Marketing of Drugs and Devices, and Protecting Human Subjects

Concern for outside review of medical research involving human subjects has been documented as far back as the early Nineteenth Century. It was not until 1966, however, when applicants for Public Health Service grant funding were subjected to such a requirement, that researchers were required to undergo review. Institutional Review Boards (IRB) are the bodies charged with performing the mandated screening functions by the Department of Health and Human Services (HHS) for federally funded projects and by the Food and Drug Administration (FDA) in the drug and device approval process.

This paper explores an IRB's responsibilities under the HHS and FDA regulations generally, and emphasizes several critical attendant issues including: the manufacturer's IRB; IRB members' individual liability; compensation for research injuries; civil rights protections afforded subjects; impact upon academic and health practitioner research; and effect upon drug and device approvals.