In vivo/ex vivo cellular interactions with titanium and copper

Machined, commercially pure titanium (Ti) disks were coated with approximately 400 nm copper (Cu) by physical vapor deposition or left uncoated. The kinetics of inflammatory cell recruitment, distribution and viability was evaluated around Ti, Cu, and in sham sites after 1, 3, 12, 18, 24, and 48 h in a rat subcutaneous (s.c.) model. Further analysis of the cells on implant surfaces was performed by ex vivo incubation of the disks. Ti and Cu stimulated an increased recruitment of inflammatory cells in comparison with sham sites. A markedly higher amount of cells, predominantly polymorpho-nuclear granulocytes (PMN), was detected around Cu after 18 h and onwards. More cells were found at the implant surfaces than in the surrounding exudates after 18 h. The total amount of lactate dehydrogenase (LDH), an indicator of plasma membrane injury, was higher in Cu exudates after 18 h in comparison with Ti and sham. In contrast, no differences in the proportion of dead cells (trypan blue dye uptake) were detected in the exudates. Further, LDH levels were higher around Ti than Cu during the initial 18 h of ex vivo incubation. The results of this study indicate that the early inflammatory process associated with a cytotoxic material in soft tissues is largely attributed to the induction of a markedly strong and prolonged chemotactic response. In contrast, this process is characterized by a higher amount of inflammatory cells around a biocompatible material than in sham sites, but with a transient course and total LDH similar to sham sites.     

Docking of sulfonamides to carbonic anhydrase II and IV

Starting with a known active site of a protein and a database of compounds, one would like to quickly identify a few compounds that "dock" into the active site and obtain "good" binding free energies. The main goal of current automated docking procedures is to predict the "best" substrate-enzyme complex while other programs such as UHBD and DelPhi can be used to compute binding free energies. In this paper, we will focus on the application of docking methods and parameters to study substrate-enzyme interactions of a metalloenzyme system. Specifically, we report on the docking of sulfonamides to carbonic anhydrase II and IV, which are of interest due to their application in glaucoma therapy. Using a standard docking protocol, it is possible to correctly predict not only the orientation of inhibitors to a specific isozyme, but also determine the qualitative affinity for a group of inhibitor for an isozyme.     

Production and characterization of transparent MgAl2O4 prepared by hot pressing

Hot pressing has been investigated for the production of transparent MgAl₂O₄ aimed at the scaling up of the process. Other assessed techniques (hot isostatic pressing, spark plasma sintering) can hardly be used for the production of flat components with large dimensions and good planarity.Hot pressing of stoichiometric Al₂O₃–MgO powder mixtures has been preferred to the direct pressing of spinel powder for the readily availability of pure powders and to exploit the thermodynamic driving force of the spinel formation. LiF has been used as sintering additive.A thermodynamic investigation of the reactions involving LiF, MgO and Al₂O₃ has helped in the comprehension of the densification mechanisms affecting the transparency of spinel. Transparencies up to 70% in the visible range (highest value 78% at 1100 nm) have been obtained. Suitable soakings have been added for promoting the initial liquid phase sintering and the release of LiF through formation of vapour phases.     

Experimental investigation of water droplet–air flow interaction in a non-reacting PEM fuel cell channel

It has been well documented that water production in PEM fuel cells occurs in discrete locations, resulting in the formation and growth of discrete droplets on the gas diffusion layer (GDL) surface within the gas flow channels (GFCs). This research uses a simulated fuel cell GFC with three transparent walls in conjunction with a high speed fluorescence photometry system to capture videos of dynamically deforming droplets. Such videos clearly show that the droplets undergo oscillatory deformation patterns. Although many authors have previously investigated the air flow induced droplet detachment, none of them have studied these oscillatory modes. The novelty of this work is to process and analyze the recorded videos to gather information on the droplets induced oscillation. Plots are formulated to indicate the dominant horizontal and vertical deformation frequency components over the range of sizes of droplets from formation to detachment. The system is also used to characterize droplet detachment size at a variety of channel air velocities. A simplified model to explain the droplet oscillation mechanism is provided as well.     

Transglutaminase-synthesized spermine derivative of substance P recognizes rat portal vein neurokinin-3 receptors

This study presents the investigation of the relations between sensation seeking (41) and anhedonia in 80 subjects who practice a risk taking sport, bengy jump. These relations were examined according to different variables which characterize the practice of bengy jump: the total number of previous jumps and the motivations to jump (1. personal motivation, 2. professional motivation--working in the jumping organization, and 3. group motivation--for example being leaded to jump by friends). We also assessed the use of psychoactive substances. All jumpers (men and women) have higher scores than control subjects in Thrill and adventure seeking dimension. In men, the more the subjects score on this dimension, the less they score on the physical anhedonia scale (8). In women, Experience Seeking and Boredom Susceptibility dimensions are positively correlated with physical anhedonia and with the number of jumps. Moreover, women with personal motivation are those who score the highest on Thrill and Adventure Seeking and Boredom Susceptibility dimensions. These results suggest that in women, sensation seeking through bengy jumping may be linked to a boredom tendency and driven by a need to compensate for a difficulty in experiencing emotions and sensations from common and mundane stimuli. In men, high frequency of bengy jumping was negatively related to the use of psychoactive substances, suggesting that bengy jump, in a compulsive form recalling an addictive behavior, is a specific and preferential way of stimulation seeking.     

Fractal behaviour of some geological media

 This paper describes the use of fractal geometry in determining the characteristics of some geological media, such as clayey and pumice soils. Attention is drawn to the determination of the specific surface of some clays of southern Italy and of pumice of the Vesuvius eruption of 79 a.d. The analysis proposed gives the Minkowski dimension of soils expressed by the power law, or the equation M_δ(F)≈Cδ^–s; this is a dimensionless number. The values of Minkowski dimension obtained have been compared with the values obtained by physical methods (mercury porosimetry and the bottle helium test). The analyses conducted prove that, for the range of magnification adopted, the media examined behave as irregular or fractal sets. Received: 10 July 1999 · Accepted: 8 October 1999     

Kuwanon‐L as a New Allosteric HIV‐1 Integrase Inhibitor: Molecular Modeling and Biological Evaluation

HIV‐1 integrase (IN) active site inhibitors are the latest class of drugs approved for HIV treatment. The selection of IN strand‐transfer drug‐resistant HIV strains in patients supports the development of new agents that are active as allosteric IN inhibitors. Here, a docking‐based virtual screening has been applied to a small library of natural ligands to identify new allosteric IN inhibitors that target the sucrose binding pocket. From theoretical studies, kuwanon‐L emerged as the most promising binder and was thus selected for biological studies. Biochemical studies showed that kuwanon‐L is able to inhibit the HIV‐1 IN catalytic activity in the absence and in the presence of LEDGF/p75 protein, the IN dimerization, and the IN/LEDGF binding. Kuwanon‐L also inhibited HIV‐1 replication in cell cultures. Overall, docking and biochemical results suggest that kuwanon‐L binds to an allosteric binding pocket and can be considered an attractive lead for the development of new allosteric IN antiviral agents.     

Microwave-assisted polyol synthesis of sub-micrometer Y2O3 and Yb-Y2O3 particles for laser source application

Sub-micrometer powder (100–150 nm diameter) of Yb-doped yttrium oxide was obtained, for the first time, by microwave-assisted polyol (diethylene glycol, DEG) method. This method is based on fast and homogeneous increase of temperature, due to the microwave heating, and on addition of the hydrolysing agent (water) at high temperature. This promotes a fast nucleation followed by a controlled growth of nuclei. Different procedures were used to process the as-synthesized powders. In some cases washing by ultrapure water was used to dissolve nitrate and DEG by-products, this treatment allowed the use of a lower calcination temperature (150–200 °C less) to obtain the crystalline phase. Analysis of the calcined powder showed different levels of structures: from nanocrystal (10–15 nm), to primary particles (100–150 nm), to micrometer soft aggregates (2–4 μm). The microwave-assisted polyol method resulted an easy way to dope yttria with the desired amount of Yb³⁺. This work was carried out in order to prepare particles to be used as rare-earth doped Y₂O₃ and YAG polycrystalline transparent ceramic for laser source applications.     

Deployment of RSS-Based Indoor Positioning Systems

Location estimation based on Received Signal Strength (RSS) is the prevalent method in indoor positioning. For such positioning systems, a massive collection of training samples is needed for their calibration. The accuracy of these methods is directly related to the placement of the reference points and the radio map used to compute the device location. Traditionally, deploying the reference points and building the radio map require human intervention and are extremely time-consuming. In this paper we present an approach to reduce the manual calibration efforts needed to deploy an RSS-based localization system, both when using only one RF technology or when using a combination of RF technologies. It is an automatic approach both to build a radio map in a given workspace by means of a signal propagation model, and to assess the system calibration that best fits the required accuracy by using a multi-objective genetic algorithm.