全文获取类型
收费全文 | 7458篇 |
免费 | 450篇 |
国内免费 | 14篇 |
学科分类
工业技术 | 7922篇 |
出版年
2023年 | 54篇 |
2022年 | 51篇 |
2021年 | 161篇 |
2020年 | 160篇 |
2019年 | 134篇 |
2018年 | 179篇 |
2017年 | 196篇 |
2016年 | 252篇 |
2015年 | 167篇 |
2014年 | 270篇 |
2013年 | 603篇 |
2012年 | 449篇 |
2011年 | 545篇 |
2010年 | 367篇 |
2009年 | 370篇 |
2008年 | 500篇 |
2007年 | 425篇 |
2006年 | 369篇 |
2005年 | 304篇 |
2004年 | 280篇 |
2003年 | 241篇 |
2002年 | 201篇 |
2001年 | 124篇 |
2000年 | 101篇 |
1999年 | 113篇 |
1998年 | 115篇 |
1997年 | 102篇 |
1996年 | 101篇 |
1995年 | 92篇 |
1994年 | 108篇 |
1993年 | 78篇 |
1992年 | 63篇 |
1991年 | 51篇 |
1990年 | 46篇 |
1989年 | 63篇 |
1988年 | 47篇 |
1987年 | 24篇 |
1986年 | 29篇 |
1985年 | 40篇 |
1984年 | 40篇 |
1983年 | 25篇 |
1982年 | 25篇 |
1981年 | 41篇 |
1980年 | 24篇 |
1979年 | 22篇 |
1978年 | 24篇 |
1977年 | 14篇 |
1975年 | 11篇 |
1974年 | 21篇 |
1973年 | 14篇 |
排序方式: 共有7922条查询结果,搜索用时 46 毫秒
991.
Stutts J Feaganes J Reinfurt D Rodgman E Hamlett C Gish K Staplin L 《Accident; analysis and prevention》2005,37(6):602-1101
Unobtrusive video camera units were installed in the vehicles of 70 volunteer drivers over 1-week time periods to study drivers’ exposure to distractions. The video data were coded based on a detailed taxonomy of driver distractions along with important contextual variables and driving performance measures. Results show distractions to be a common component of everyday driving. In terms of overall event durations, the most common distractions were eating and drinking (including preparations to eat or drink), distractions inside the vehicle (reaching or looking for an object, manipulating vehicle controls, etc.), and distractions outside the vehicle (often unidentified). Distractions were frequently associated with decreased driving performance, as measured by higher levels of no hands on the steering wheel, eyes directed inside rather than outside the vehicle, and lane wanderings or encroachments. Naturalistic driving studies can provide a useful supplement to more controlled laboratory and field studies to further our understanding of the effects of all types of distractions on driving safety. 相似文献
992.
An optimal system for temperature measurements by coherent anti-Stokes Raman spectroscopy (CARS) in turbulent flames and flows is presented. In addition to a single-mode pump laser and a modeless dye laser, an echelle spectrometer with a cross disperser is used. This system permits simultaneous measurement of the N2 CARS spectrum and the broadband dye laser profile. A procedure is developed to use software to transform this profile into the excitation profile by which the spectrum is referenced. Simultaneous shot-to-shot referencing is compared to sequential averaged referencing for data obtained in flat flames and in room air. At flame temperatures, the resultant 1.5% imprecision is limited by flame fluctuations, indicating that the system may have a single-shot imprecision below 1%. At room temperature, the 3.8% single-shot imprecision is of the same order as the best values reported for dual-broadband pure-rotational CARS. Using the unique shot-to-shot excitation profiles, simultaneous referencing eliminates systematic errors. At 2000 and 300 K, the 95% confidence intervals are estimated to be +/- 20 and +/- 10 K, respectively. 相似文献
993.
An atmospheric pressure matrix-assisted laser desorption/ionization (AP MALDI) source coupled to Fourier transform ion cyclotron resonance mass spectrometry (FT ICR MS) under UV laser and solid matrix conditions has been demonstrated to analyze a variety of labile oligosaccharides including O-linked and N-linked complex glycans released from glycoproteins. Spectra were acquired by both AP MALDI and vacuum MALDI and directly compared. The results presented here confirm that AP MALDI can generate significantly less energetic ions than vacuum MALDI and is able to produce the intact molecular ions with little or no fragmentation in both positive and negative ion mode analyses. Under certain conditions, noncovalent complexes of sialylated oligosaccharides were observed. The sensitivity attainable by AP MALDI was found to be comparable to conventional MALDI, and tandem mass spectrometry of oligosaccharides ionized by AP MALDI was shown to allow detailed structural analysis. Analysis of N-glycan mixtures derived from human fibrinogen further demonstrated that AP MALDI-FT ICR MS is ideal for the study of complex glycan samples as it provides high-accuracy, high-resolution mass analysis with no difficulty in distinguishing sample constituents from fragment ions. 相似文献
994.
Russell SC Czerwieniec G Lebrilla C Steele P Riot V Coffee K Frank M Gard EE 《Analytical chemistry》2005,77(15):4734-4741
Bioaerosol mass spectrometry (BAMS) performs single-cell analysis in real time. However, the specificity of BAMS mass signatures has been limited by low sensitivity at high masses. To increase the mass range and sensitivity of BAMS, a novel design was developed that utilizes a linear flight tube with delayed extraction and an electrostatic ion guide. This study quantifies the sensitivity limits of the novel BAMS design and evaluates the feasibility of BAMS to detect higher mass biomarkers from single cells. All experiments were carried out using MALDI aerosol particles that were nebulized from solution. Sensitivity was assessed by generating particles with decreasing amounts of analyte via serial dilutions. The amount of analyte contained within each particle was calculated based on particle size, density, and molarity of the analyte within solution. A variety of biomolecular ions were studied and signals obtained from particles containing 300 zmol of maltopentaose, 132 zmol of alpha-cyclodextrin, and 14 zmol (approximately 8400 molecules) of gramicidin S are reported. The detection of 14 zmol of gramicidin S is to the best of our knowledge a record in sensitivity for MALDI TOF-MS. 相似文献
995.
Steele PT Srivastava A Pitesky ME Fergenson DP Tobias HJ Gard EE Frank M 《Analytical chemistry》2005,77(22):7448-7454
Bioaerosol mass spectrometry is being developed to analyze and identify biological aerosols in real time. Mass spectra of individual Bacillus endospores were measured with a bipolar aerosol time-of-flight mass spectrometer in which molecular desorption and ionization were produced using a single laser pulse from a Q-switched, frequency-quadrupled Nd:YAG laser that was modified to have an approximately flattop profile. The flattened laser profile allowed the minimum fluence required to desorb and ionize significant numbers of ions from single aerosol particles to be determined. For Bacillus spores, this threshold had a mean value of approximately 1 nJ/microm(2) (0.1 J/cm(2)). Thresholds for individual spores, however, could apparently deviate by 20% or more from the mean. Threshold distributions for clumps of MS2 bacteriophage and bovine serum albumin were subsequently determined. Finally, the flattened profile was observed to increase the reproducibility of single-spore mass spectra. This is consistent with the general conclusions of our earlier paper on the fluence dependence of single-spore mass spectra and is particularly significant because it is expected to enable more robust differentiation and identification of single bioaerosol particles. 相似文献
996.
Supported liquid membranes are used here to establish steady-state concentration profiles across ion-selective membranes rapidly and reproducibly. This opens up new avenues in the area of nonequilibrium potentiometry, where reproducible accumulation and depletion processes at ion-selective membranes may be used to gain valuable analytical information about the sample. Until today, drifting signals originating from a slowly developing concentration profile across the ion-selective membrane made such approaches impractical in zero current potentiometry. Here, calcium- and silver-selective membranes were placed between two identical aqueous electrolyte solutions, and the open circuit potential was monitored upon changing the composition of one solution. Steady state was reached in approximately 1 min with 25-microm porous polypropylene membranes filled with bis(2-ethylhexyl) sebacate doped with ionophore and lipophilic ion exchanger. Ion transport across the membrane resulted on the basis of nonsymmetric ion-exchange processes at both membrane sides. The steady-state potential was calculated as the sum of the two membrane phase boundary potentials, and good correspondence to experiment was observed. Concentration polarizations in the contacting aqueous phases were confirmed with stirring experiments. It was found that interferences (barium in the case of calcium electrodes and potassium with silver electrodes) induce a larger potential change than expected with the Nicolsky equation because they influence the level of polarization of the primary ion (calcium or silver) that remains potential determining. 相似文献
997.
Genetic strain variations in the metaphase-II phenotype of mouse oocytes matured in vivo or in vitro
Ibáñez E Sanfins A Combelles CM Overström EW Albertini DF 《Reproduction (Cambridge, England)》2005,130(6):845-855
The interplay between genetic and epigenetic factors plays a central role in mammalian embryo production strategies that superimpose ex vivo or in vivo manipulations upon strain background characteristics. In this study, we examined the relationship between genetic background and the phenotypic properties of mouse metaphase-II (M-II) oocytes that were matured under in vivo (IVO) or in vitro conditions, either in a basal (IVM) or a supplemented (IVM + ) medium. Differences existed amongst inbred (C57BL/6), outbred (CF-1, Black Swiss, NU/NU) and hybrid lines (B6D2F1) induced to superovulate with regard to cytoplasmic microtubule organizing center (MTOC) number but not spindle size or shape, except for larger and asymmetrical spindles in Black Swiss oocytes. When oocytes were matured in culture, meiotic spindle and cytoplasmic phenotypic properties of M-II oocytes were affected relative to in vivo conditions and between strains. Specifically, measures of meiotic spindle size, shape, polar pericentrin distribution and cytoplasmic MTOC number all revealed characteristic variations. Interestingly, the overall reduction in cytoplasmic MTOC number noted upon IVM was concomitant with an overall increase in spindle and polar body size. Maturation under IVM + conditions resulted in a further decrease in cytoplasmic MTOC number, but spindle and polar body characteristics were intermediate between IVO and IVM. How these oocyte phenotypic properties of maternal origin may be linked to predictive assessments of fecundity remains to be established. 相似文献
998.
Studies in cell culture and laboratory animals have shown that green tea and its major component, epigallocatechin-3-gallate, inhibit cell growth and reduce tumor incidence. However, results of epidemiological studies have generated inconsistent, sometimes conflicting data regarding protection by green tea against human cancers. To clarify the findings of these laboratory studies in application to humans, we conducted a pilot intervention study with three heavy smokers (> 10 cigarettes/day) and three nonsmokers (never smokers) in order to evaluate the molecular and cellular effects of drinking green tea using human oral cells as an investigative tool. Green tea total extract (400-500 mg/cup, 5 cups/day) was administered in drinking water to the subjects for four weeks. Two oral cytology samples were taken weekly for measurements of tobacco carcinogen-induced DNA damage, including bulky adducts and oxidized bases, cell growth, DNA content, and apoptosis. The study showed that during the course of green tea administration smoking-induced DNA damage was decreased, cell growth was inhibited, and the percentage of cells in S phase was reduced, cells accumulated in G1 phase (cyclin D1 positive), DNA content became more diploid and less aneuploid, and p53, Caspase-3, and TUNEL, markers of apoptosis, were increased. The study, although preliminary, indicates that drinking green tea reduced the number of damaged cells in smokers by inducing cell growth arrest and apoptosis, a mechanism similar to that observed in cultured cells and animals. These results warrant a large-scale intervention trial to further verify the role of green tea in the prevention of oral cancer in smokers. 相似文献
999.
Lemarchand E Dormieux L Ulm FJ 《Philosophical transactions. Series A, Mathematical, physical, and engineering sciences》2005,363(1836):2581-2602
Expansive reactions damage porous materials through the formation of reaction products of a volume in excess of the available space left by the reactants and the natural porosity of the material. This leads to pressurizing the pore space accessible to the reaction products, which differs when the chemical reaction is through-solution or topochemical or both in nature. This paper investigates expansive reactions from a micromechanical point of view, which allows bridging the scale from the local chemo-mechanical mechanisms to the macroscopically observable stress-free expansion. In particular, the study of the effect of morphology of the pore space, in which the chemical expansion occurs locally, on the macroscopically observable expansion is the main focus of this paper. The first part revisits the through-solution and the topochemical reaction mechanism within the framework of micro-macro-homogenization theories, and the effect of the microscopic geometry of pores and microcracks in the solid matrix on the macroscopic chemical expansion is examined. The second part deals with the transition from a topochemical to a through-solution-like mechanism that occurs in a solid matrix with inclusions (cracks, pores) of different morphology. 相似文献
1000.
We present fabrication routes for a new type of surface specimen that exhibits a micro pattern with a gradient in chemical contrast between the pattern domains. Design elements in the specimen allow chemical contrast in the micro pattern to be related to well-established surface characterization data, such as contact angle measurements. These gradient specimens represent a reference tool for calibrating image contrast in chemically sensitive scanning probe microscopy techniques and a platform for the high-throughput analysis of polymer thin film behavior. 相似文献