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Laboratory conditions have been identified that cause the rapid death of cultures of cyanobacteria producing urease. Once the death phase had initiated in the stationary growth phase, cells were rapidly bleached of all pigmentation. Null mutations in the ureC gene, encoding the alpha subunit of urease, were constructed, and these mutants were no longer sensitive to growth in the presence of urea. High levels of peroxides, including lipid peroxides, were detected in the bleaching cells. Exogenously added polyunsaturated fatty acids triggered a similar death response. Vitamin E suppressed the formation of peroxides and delayed the onset of cell bleaching. The results suggest that these cyanobacterial cells undergo a metabolic imbalance that ultimately leads to oxidative stress and lipid peroxide formation. These observations may provide insights into the mechanism of sudden cyanobacterial bloom disappearance in nature. 相似文献
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The purpose of this study was to assess factors associated with the use of family planning at first sexual intercourse among young adults aged 15 to 24 in urban Ecuador. The study population consisted of 1443 young adults (494 females and 949 males) in the cities of Quito and Guayaquil, interviewed by the 1988 Ecuador Young Adult Reproductive Health Survey, who reported having experienced consensual sexual intercourse. Approximately 11% of females and 15% of males reported using contraception at first intercourse. Binary logistic regression was performed to assess jointly the effect of multiple factors on contraceptive use at first intercourse. The regression model was first run on the entire study population and then separately for males and females. In the overall population, the following variables were significantly related to using family planning at first sex: being male; being from Guayaquil; older age; father's completion of secondary school. Having lost one's virginity to a prostitute was significantly associated with non-use of family planning. Males were 3.6 times more likely than females to use family planning during their first sexual intercourse. For each year older a young adult was at first sex, his or her odds of using family planning was multiplied by a factor of 1.3. Twenty-eight per cent of males in this study experienced their first sexual intercourse with a prostitute, and these young men were highly unlikely to use family planning. A male who experienced first intercourse with his girlfriend was more than five times as likely to use contraception than a male who lost his virginity to a prostitute. 相似文献
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Jetten Jolanda; Spears Russell; Manstead Antony S. R. 《Canadian Metallurgical Quarterly》1998,74(6):1481
We tested the prediction, derived from an integration of social identity and self-categorization principles, that the relation between in-group distinctiveness and positive differentiation is curvilinear. Moderate distinctiveness is argued to provide the critical combination of intergroup difference (self-categorization theory) and intergroup similarity ( social identity theory ) to elicit intergroup differentiation. Intergroup distance (differences in group central tendencies) and group variability were manipulated orthogonally, resulting in different levels of intergroup distinctiveness (low, intermediate, and high). In line with predictions, in-group bias and positive differentiation were greatest, for both minimal and natural groups, when the combination of group variability and intergroup distance produced intermediate levels of group distinctiveness. The potential of this integrative model to account for disparate findings is discussed. (PsycINFO Database Record (c) 2011 APA, all rights reserved) 相似文献
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Antony D. Radford 《Computer aided design》1981,13(6):345-350
Designing windows in buildings is a multicriteria, multivariable design problem with many constraints. This paper examines the different natures of the window design information that can be provided through mathematical simulation and optimization models and considers some approaches to choosing a solution. 相似文献
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