全文获取类型
收费全文 | 4019篇 |
免费 | 185篇 |
国内免费 | 17篇 |
学科分类
医药卫生 | 4221篇 |
出版年
2023年 | 25篇 |
2022年 | 14篇 |
2021年 | 64篇 |
2020年 | 55篇 |
2019年 | 48篇 |
2018年 | 70篇 |
2017年 | 68篇 |
2016年 | 75篇 |
2015年 | 66篇 |
2014年 | 92篇 |
2013年 | 100篇 |
2012年 | 216篇 |
2011年 | 216篇 |
2010年 | 116篇 |
2009年 | 125篇 |
2008年 | 211篇 |
2007年 | 212篇 |
2006年 | 199篇 |
2005年 | 237篇 |
2004年 | 229篇 |
2003年 | 217篇 |
2002年 | 223篇 |
2001年 | 101篇 |
2000年 | 119篇 |
1999年 | 112篇 |
1998年 | 45篇 |
1997年 | 47篇 |
1996年 | 43篇 |
1995年 | 33篇 |
1994年 | 31篇 |
1993年 | 49篇 |
1992年 | 88篇 |
1991年 | 76篇 |
1990年 | 82篇 |
1989年 | 52篇 |
1988年 | 49篇 |
1987年 | 54篇 |
1986年 | 39篇 |
1985年 | 46篇 |
1984年 | 37篇 |
1983年 | 25篇 |
1981年 | 14篇 |
1979年 | 29篇 |
1978年 | 13篇 |
1977年 | 14篇 |
1976年 | 15篇 |
1975年 | 23篇 |
1974年 | 12篇 |
1971年 | 11篇 |
1969年 | 13篇 |
排序方式: 共有4221条查询结果,搜索用时 22 毫秒
71.
72.
Severe exacerbation of hepatitis after short-term corticosteroid therapy in a patients with "latent" chronic hepatitis B 总被引:2,自引:0,他引:2
Shiota G Harada K Oyama K Udagawa A Nomi T Tanaka K Tsutsumi A Noguchi N Kishimoto Y Horie Y Suou T Kawasaki H 《Liver》2000,20(5):415-420
We present a case of severe exacerbation of hepatitis after short-term corticosteroid therapy for chronic inflammatory demyelinating polyneuropathy (CIPD) with "latent" chronic hepatitis B showing no HBV-related antigens and antibodies. After corticosteroid pulse therapy for CIPD, the patient had severe exacerbation of hepatitis twice. Although she did not show any hepatitis B virus (HBV)-related antigens or antibodies, sequences of HBV were detected in serum and liver by a nested polymerase chain reaction. A sequence analysis of HBV at the second exacerbation showed that the G-to-A point mutation at nucleotide 1896 that converted codon 28 from tryptophan (TGG) to a stop codon (TAG) in the precore region resulted in amino acid change, which has been frequently observed in fulminant hepatitis and severe hepatitis in Japan. 相似文献
73.
Haruna T Yoshida H Nakamura TY Xie LH Otani H Ninomiya T Takano M Coetzee WA Horie M 《Circulation research》2002,91(3):232-239
Phosphatidylinositol 4,5-bisphosphate (PIP2) stimulates ATP-sensitive K+ (K(ATP)) channel activity. Because phospholipase C (PLC) hydrolyzes membrane-bound PIP2, which in turn may potentially decrease K(ATP) channel activity, we investigated the effects of the alpha1-adrenoceptor-G(q)-PLC signal transduction axis on pinacidil-activated K(ATP) channel activity in adult rat and neonatal mouse ventricular myocytes. The alpha1-adrenoceptor agonist methoxamine (MTX) reversibly inhibited the pinacidil-activated K(ATP) current in a concentration-dependent manner (IC50 20.9+/-6.6 micromol/L). This inhibition did not occur when the specific alpha1-adrenoceptor antagonist, prazosin, was present. An involvement of G proteins is suggested by the ability of GDPbetaS to prevent this response. Blockade of PLC by U-73122 (2 micromol/L) or neomycin (2 mmol/L) attenuated the MTX-induced inhibition of K(ATP) channel activity. In contrast, the MTX response was unaffected by protein kinase C inhibition or stimulation by H-7 (100 micro mol/L) or phorbol 12,13-didecanoate. The MTX-induced inhibition became irreversible in the presence of wortmannin (20 micro mol/L), an inhibitor of phosphatidylinositol-4 kinase, which is expected to prevent membrane PIP2 replenishment. In excised inside-out patch membranes, pinacidil induced a significantly rightward shift of ATP sensitivity of the channel. This phenomenon was reversed by pretreatment of myocytes with MTX. Direct visualization of PIP2 subcellular distribution using a PLCdelta pleckstrin homology domain-green fluorescent protein fusion constructs revealed reversible translocation of green fluorescent protein fluorescence from the membrane to the cytosol after alpha1-adrenoceptor stimulation. Our data demonstrate that alpha1-adrenoceptor stimulation reduces the membrane PIP2 level, which in turn inhibits pinacidil-activated K(ATP) channels. 相似文献
74.
Miyashita N Fukano H Hara H Yoshida K Niki Y Matsushima T 《Internal medicine (Tokyo, Japan)》2002,41(1):30-33
Two cases of recurrent pneumonia due to Chlamydia pneumoniae are described. C. pneumoniae was continuously detected from the nasopharynx in both patients by the polymerase chain reaction and/or culture even with appropriate antibiotic therapy during the first episode. After eradication of C. pneumoniae with long-term macrolide therapy, the respiratory symptoms of both patients completely disappeared and no relapse was observed. These data indicate that new treatment strategies may be necessary to eradicate the organism in patients prone to persistent infection. 相似文献
75.
Makoto Seki Akio Yanagisawa Eiji Ninomiya Yasuro Ninomiya Hirotoshi Ohta Akio Saiura Junji Yamamoto Toshiharu Yamaguchi Akiko Aruga Keiko Yamada Koichi Takano Rikiya Fujita Masayuki Ikeda Keiko Sasaki Yo Kato 《Journal of hepato-biliary-pancreatic sciences》2005,12(3):254-262
Background/Purpose
Between 1988 and 2003, 38 patients underwent biliary resection for pancreaticobiliary maljunction (PBM). We reviewed the histopathologic findings for the surgically resected specimens to compare the clinical and pathologic features and assess the relationship between changes in the background biliary epithelium and the development of neoplasms.Methods
Papillary hyperplasia (PHP) seen in the biliary epithelium of patients with PBM, was classified into grades 0–III in the gallbladder and grades 0–II in the extrahepatic bile duct, according to the extent, and was assessed for links with tumors in the same specimens.Results
The incidence of gallbladder carcinoma was 13/21 in grades I–II, versus 0/16 in grade III, while the incidence of bile duct carcinoma was 4/20 in grade I versus 0/5 in grade II. Furthermore, these incidences for patients below age 50 years and age 50 or older were 1/18 versus 12/20, and 0/14 versus 6/17, respectively.Conclusions
PHP of the biliary epithelium in PBM patients is an important precursor lesion, especially for gallbladder cancer, and the risk becomes greater with age, regardless of the type of pancreatobiliary junction (PBJ) and its location in the biliary tract. 相似文献76.
Low-dose ethanol attenuates gut ischemia/reperfusion-induced liver injury in rats via nitric oxide production 总被引:2,自引:0,他引:2
Horie Y Yamagishi Y Kato S Kajihara M Kimura H Ishii H 《Journal of gastroenterology and hepatology》2003,18(2):211-217
BACKGROUND AND AIMS: The acute administration of low-dose ethanol was demonstrated to attenuate liver injury elicited by gut ischemia/reperfusion (I/R). Nitric oxide (NO) has been found to be a modulator of adhesive interactions between leukocytes, platelets, and endothelial cells, but there has been much controversy about the effects of ethanol on NO regulation. The objective of this study was to investigate the role of NO in ethanol-reduced hepatic microvascular dysfunction elicited by gut I/R. METHODS: Male Wistar rats were exposed to 30 min of gut ischemia followed by 60 min of reperfusion. Intravital microscopy was used to monitor leukocyte recruitment and non-perfused sinusoids (NPS). Plasma alanine aminotransferase (ALT) activities were measured 6 h after the onset of reperfusion. In another set of experiments, ethanol (10%, 1 g/kg) was administered before ischemia. RESULTS: Gut I/R elicited increases in the number of stationary leukocytes, NPS, and plasma ALT activities; all of which were attenuated by pretreatment with ethanol or an NO donor. Gut I/R caused the apoptosis of hepatocytes, which was prevented by pretreatment with ethanol. Pretreatment with an NO synthase inhibitor diminished the protective effects of ethanol. The administration of ethanol increased plasma nitrite/nitrate levels. CONCLUSION: These results suggest that low-dose ethanol attenuates the gut I/R-induced hepatic microvascular dysfunction and sequential liver injury by increasing sinusoidal NO levels. 相似文献
77.
Systemic and local evidence of increased Fas-mediated apoptosis in ulcerative colitis 总被引:10,自引:0,他引:10
Yukawa M Iizuka M Horie Y Yoneyama K Shirasaka T Itou H Komatsu M Fukushima T Watanabe S 《International journal of colorectal disease》2002,17(2):70-76
BACKGROUND AND AIMS: Recent studies suggest that Fas-mediated apoptosis is involved in the pathogenesis of inflammatory bowel disease (IBD). This study was conducted to clarify whether soluble forms of Fas (sFas) and Fas ligand (sFasL) are concerned with inflammation in IBD. METHODS AND PATIENTS: Concentration of serum sFas and sFasL was measured by enzyme-linked immunosorbent assay in 10 patients with ulcerative colitis (UC), 10 with Crohn's disease (CD) in both active and remission stages, and 20 controls. Expression of Fas and sFas in colonic mucosa was examined by western blot. Distribution of Fas and FasL in colonic mucosa was examined by immunohistochemistry in 20 UC, 20 CD, and 10 non-IBD colitis patients and in 10 controls. Apoptotic cells were examined by TUNEL. RESULTS: Concentration of systemic sFas was significantly lower in active UC than controls. The number of FasL-containing cells was significantly higher in active UC than in remission UC, non-IBD colitis, and controls. Apoptotic cells were increased in active UC. CONCLUSIONS: Our results demonstrate that systemic and local Fas-mediated apoptosis is promoted in UC, which might be involved in the pathogenesis in UC. 相似文献
78.
Goshi Shiota Ken‐ichi Harada Kenji Oyama Akihide Udagawa Takahiro Nomi Kiwamu Tanaka Atsushi Tsutsumi Naoya Noguchi Yosuke Kishimoto Yutaka Horie Takeaki Suou Hironaka Kawasaki 《Liver international》2000,20(5):415-420
Abstract: We present a case of severe exacerbation of hepatitis after short‐term corticosteroid therapy for chronic inflammatory demyelinating polyneuropathy (CIPD) with “latent” chronic hepatitis B showing no HBV‐related antigens and antibodies. After corticosteroid pulse therapy for CIPD, the patient had severe exacerbation of hepatitis twice. Although she did not show any hepatitis B virus (HBV)‐related antigens or antibodies, sequences of HBV were detected in serum and liver by a nested polymerase chain reaction. A sequence analysis of HBV at the second exacerbation showed that the G‐to‐A point mutation at nucleotide 1896 that converted codon 28 from tryptophan (TGG) to a stop codon (TAG) in the precore region resulted in amino acid change, which has been frequently observed in fulminant hepatitis and severe hepatitis in Japan. 相似文献
79.
Hypoxia‐inducible factor‐3α promotes angiogenic activity of pulmonary endothelial cells by repressing the expression of the VE‐cadherin gene 下载免费PDF全文
80.
Christiane Bergamasco RD Lilian Mika Horie RD Raquel Susana Torrinhas BD Dan L. Waitzberg MD PhD 《JPEN. Journal of parenteral and enteral nutrition》2015,39(8):941-947
Background: The daily consumption of dietary fiber is frequently below suggested recommendations. Using a double‐blind, controlled, randomized study, we assessed the efficiency and tolerance of a fiber‐enriched orange juice to supplement fiber intake in women. Materials and Methods: After 1 week of noninterventional observation, 192 healthy adult women ingested 400 mL of orange juice for 21 days, which either was not (placebo group) or was enriched with fiber (fiber group). Orange juice ingestion was registered daily and controlled for each week during the study period. Macronutrient, fiber, and energy intake were determined using a 3‐day food record, validated food chemical composition databases, and the “Pro Diet” software. Gastrointestinal symptoms were self‐evaluated daily by scoring 4 grades of symptom intensity and using a visual analog scale to grade pain severity. Results: No changes were observed for macronutrient and energy ingestion. For the placebo group (n = 97), the total fiber intake record was under the daily recommended value. In contrast, the fiber group (n = 95) displayed higher comparative values of total and soluble fiber consumption (P ≤ .001), achieving the daily recommended values of fiber intake. Both groups reported an increased frequency of slight bloating and rumbles over time (P ≤ .05). The fiber group also experienced a higher frequency of slight flatulence over time (P = .002). Conclusion: Consumption of fiber‐enriched orange juice was efficient to achieve the daily fiber intake recommendation for women, was not accompanied by intense adverse events, and may represent a suitable method to supplement fiber intake in woman. 相似文献