OLTIPRAZ对小鼠日本血吸虫病的初步治疗

Oltipraz is a new drug against Schistosomiasis mansoni and was used to treat mice Schistosorniasis japonica in our laboratory. The results showed that 48 h after oral administraction of oltipraz at a single dose of 900 mg/kg, 97% Schistosomes in mice infected with Schistosomiasis japonica schifted to the liver of the host. About half of these worms returned to the mesenteric veins in 96 h. Infected mice was given oltipraz orally at the dose of 900 mg/kg.d for 3～5 days, and killed in 28 days after the last dose. Over 95% total worm reduction rate was found.Schistosomes in infected mice treated with oltipraz were collected for histological observation. The results showed that the tegument of schistosomes were damaged and host cells invaded into the worm body; and granuloma formation of dead worm was observed.During the treatment, food uptake and body weight of the infected mice were decreased, but regained soon after the cessation of the treatment.     

麝香的药理研究 Ⅱ.麝香及其有效成分的抗炎作用

麝香水提物对小鼠巴豆油耳部炎症,大鼠琼脂性关节肿、酵母性关节肿、佐剂型多发性关节炎均具非常显著的抑制作用。对大鼠烫伤性血管渗透性增加、羧甲基纤维素引起的腹腔白细胞游走亦具非常明显的抑制作用。静脉注射麝香1号对巴豆油小鼠耳炎症的50%抑制剂量为0.63 mg/kg,为氢化可的松作用强度的36倍,如以克分子剂量相比,则为氢化可的松的500倍以上。静脉注射麝香水提物对小鼠的LD_(50)及95%可信限为848±104 mg/kg。     

Alteration and abnormal expression of the c-myc oncogene in human multiple myeloma

Structural alterations of the c-myc oncogene in human Burkitt's lymphoma and mouse plasmacytoma suggest that this oncogene is involved in several B cell neoplasms. The possibility of c-myc alterations in human myeloma has not been explored, probably because the low proliferative activity characteristic of this tumor impairs the propagation of representative cell lines for the performance of adequate cytogenetic studies. This report describes alterations in the c-myc locus with concomitant elevated expression of mRNA in the tumor cells of two of 37 patients with multiple myeloma. In one case, somatic cell hybrid studies revealed that the cloned rearranged DNA was entirely derived from chromosome 8, thus indicating a novel mechanism of c-myc activation different from that in Burkitt's lymphoma. Seven other patients exhibited five- to 12-fold overexpression of c-myc RNA when compared with normal marrow cells. Elevated mRNA expression in about one fourth of our patients suggests that the c-myc oncogene has a pathogenetic role in the evolution of multiple myeloma.     

Characterization of MR spectroscopic imaging of the human head and limb at 2.0 T

Using section-select and phase-encoding gradients, the authors obtained phosphorus chemical shift images of the human head and limb. Phosphorus spectra were acquired from planar sections divided into voxels as small as 7 cm3 in calf muscle and 27 cm3 in brain, with total examination times, including setup and proton locator imaging, of roughly 1 hour. Both spin-echo and free induction decay (FID) methods were employed; the FID gave superior results. Signal-to-noise ratios for the beta-adenosine triphosphate and phosphocreatine resonances were as high as 10:1 and 13:1 from volumes of 27 cm3 in brain.     

Characterization of the specificity and duration of T cell tolerance to intranasally administered peptides in mice: a role for intramolecular epitope suppression

Mucosal administration of antigens in experimental animals leads to the induction of peripheral T cell tolerance. We have previously reported that in H-2b mice, intranasal (i.n.) or oral administration of a peptide containing the immunodominant T cell epitope will down-regulate the function of CD4+ T cells reactive with Der P 1, a major target antigen in both B and T cell responses to house dust mite. In the present study we have investigated the tolerogenicity of peptides containing both dominant and subdominant determinants when given i.n. to nalve mice. Induction of tolerance by the nasally administered immunodominant peptide leads to a diminution in all T cell-derived cytokines and modulation of delayed-type hypersensitivity responses, but IgE production did not seem to be affected, furthermore the induction of T cell tolerance was stable, lasting beyond 6 months. We have also examined the specificity of intramolecular epitope suppression which is a feature of mucosal tolerance induced by nasally administered peptides and demonstrate that regulatory CD4+ T cells may exert their suppressive effect by linked recognition of epitopes on the same or neighbouring antigen-presenting cells.