全文获取类型
收费全文 | 3043篇 |
免费 | 273篇 |
国内免费 | 7篇 |
学科分类
医药卫生 | 3323篇 |
出版年
2023年 | 26篇 |
2022年 | 12篇 |
2021年 | 96篇 |
2020年 | 67篇 |
2019年 | 74篇 |
2018年 | 92篇 |
2017年 | 56篇 |
2016年 | 54篇 |
2015年 | 73篇 |
2014年 | 98篇 |
2013年 | 124篇 |
2012年 | 229篇 |
2011年 | 174篇 |
2010年 | 103篇 |
2009年 | 84篇 |
2008年 | 150篇 |
2007年 | 167篇 |
2006年 | 161篇 |
2005年 | 147篇 |
2004年 | 134篇 |
2003年 | 132篇 |
2002年 | 113篇 |
2001年 | 75篇 |
2000年 | 64篇 |
1999年 | 64篇 |
1998年 | 29篇 |
1997年 | 22篇 |
1996年 | 13篇 |
1995年 | 24篇 |
1994年 | 25篇 |
1993年 | 22篇 |
1992年 | 40篇 |
1991年 | 60篇 |
1990年 | 36篇 |
1989年 | 54篇 |
1988年 | 35篇 |
1987年 | 33篇 |
1986年 | 37篇 |
1985年 | 38篇 |
1984年 | 34篇 |
1983年 | 33篇 |
1981年 | 21篇 |
1980年 | 21篇 |
1979年 | 27篇 |
1978年 | 20篇 |
1977年 | 15篇 |
1974年 | 11篇 |
1973年 | 18篇 |
1972年 | 10篇 |
1970年 | 12篇 |
排序方式: 共有3323条查询结果,搜索用时 15 毫秒
131.
Juliette K. Tinker Jie Yan Reece J. Knippel Panos Panayiotou Kenneth A. Cornell 《Toxins》2014,6(4):1397-1418
West Nile virus (WNV) causes potentially fatal neuroinvasive disease and persists at endemic levels in many parts of the world. Despite advances in our understanding of WNV pathogenesis, there remains a significant need for a human vaccine. The domain III (DIII) region of the WNV envelope protein contains epitopes that are the target of neutralizing antibodies. We have constructed a chimeric fusion of the non-toxic cholera toxin (CT) CTA2/B domains to DIII for investigation as a novel mucosally-delivered WNV vaccine. Purification and assembly of the chimera, as well as receptor-binding and antigen delivery, were verified by western blot, GM1 ELISA and confocal microscopy. Groups of BALB/c mice were immunized intranasally with DIII-CTA2/B, DIII, DIII mixed with CTA2/B, or CTA2/B control, and boosted at 10 days. Analysis of serum IgG after 14 and 45 days revealed that mucosal immunization with DIII-CTA2/B induced significant DIII-specific humoral immunity and drove isotype switching to IgG2a. The DIII-CTA2/B chimera also induced antigen-specific IgM and IgA responses. Bactericidal assays indicate that the DIII-CTA2/B immunized mice produced DIII-specific antibodies that can trigger complement-mediated killing. A dose escalation resulted in increased DIII-specific serum IgG titers on day 45. DIII antigen alone, in the absence of adjuvant, also induced significant systemic responses after intranasal delivery. Our results indicate that the DIII-CTA2/B chimera is immunogenic after intranasal delivery and merits further investigation as a novel WNV vaccine candidate. 相似文献
132.
Alexandre Janin Valrie Chanavat Pierre‐Antoine Rollat‐Farnier Claire Bardel Karine Nguyen Philippe Chevalier Jean‐Christophe Eicher Laurence Faivre Juliette Piard Emma Albert Severine Nony Gilles Millat 《Human mutation》2020,41(2):465-475
Hypertrophic cardiomyopathy (HCM) is the most common heritable cardiomyopathy, historically believed to affect 1 of 500 people. MYBPC3 pathogenic variations are the most frequent cause of familial HCM and more than 90% of them introduce a premature termination codon. The current study aims to determine the prevalence of deep intronic MYBPC3 pathogenic variations that could lead to splice mutations. To improve molecular diagnosis, a next‐generation sequencing (NGS) workflow based on whole MYBPC3 sequencing of a cohort of 93 HCM patients, for whom no putatively causative point mutations were identified after NGS sequencing of a panel of 48 cardiomyopathy‐causing genes, was performed. Our approach led us to reconsider the molecular diagnosis of six patients of the cohort (6.5%). These HCM probands were carriers of either a new large MYBPC3 rearrangement or splice intronic variations (five cases). Four pathogenic intronic variations, including three novel ones, were detected. Among them, the prevalence of one of them (NM_000256.3:c.1927+ 600 C>T) was estimated at about 0.35% by the screening of 1,040 unrelated HCM individuals. This study suggests that deep MYBPC3 splice mutations account for a significant proportion of HCM cases (6.5% of this cohort). Consequently, NGS sequencing of MYBPC3 intronic sequences have to be performed systematically. 相似文献
133.
134.
Hélène Louis-Dit-Picard Ilektra Kouranti Chloé Rafael Irmine Loisel-Ferreira Maria Chavez-Canales Waed Abdel-Khalek Eduardo R. Argaiz Stéphanie Baron Sarah Vacle Tiffany Migeon Richard Coleman Marcio Do Cruzeiro Marguerite Hureaux Nirubiah Thurairajasingam Stéphane Decramer Xavier Girerd Kevin O’Shaugnessy Paolo Mulatero Gwena?lle Roussey Ivan Tack Robert Unwin Rosa Vargas-Poussou Olivier Staub Richard Grimm Paul A. Welling Gerardo Gamba Eric Clauser Juliette Hadchouel Xavier Jeunemaitre 《The Journal of clinical investigation》2020,130(12):6379
135.
136.
137.
138.
Justin Pulford Stella Black Amanda Wheeler Janie Sheridan Peter Adams 《International journal of mental health and addiction》2010,8(3):493-502
This paper examines the post-treatment support practices, attitudes and preferences of outpatient alcohol and other drug (AOD)
treatment staff as well as perceived barriers to implementing a post-treatment support service in an outpatient AOD treatment
context. Data were collected via semi-structured interview and group discussion (n = 23). Findings suggest that post-treatment support was rarely provided by participating AOD treatment staff or their respective
services. However, there was widespread support for implementing such services, and it was generally believed that implementation
would be most successful if: multiple post-treatment support options were made available; if one or more of these options
were based on the maintenance of an established client/clinician relationship; and if one of the options involved proactive
(service-led) telephone support. A number of barriers to possible implementation were identified, although none were considered
insurmountable. 相似文献
139.
Michael A. Caruso Jeffery D. Kittilson Mark A. Sheridan 《General and comparative endocrinology》2010,169(3):258-268
Insulin (INS) plays a critical role in the growth, development, and metabolism of vertebrates. In this study, a cDNA encoding a novel insulin receptor (IR) subtype was isolated, cloned, and sequenced from the liver of rainbow trout. A 1525-bp cDNA encoding a partial amino acid sequence of the β-subunit including the transmembrane domain, the tyrosine kinase domain, and the 3′ untranslated region (UTR) was obtained and designated IR2 based on comparison with known IR subtypes, including the three previously reported IR subtypes of trout. Trout IR2 shares 90.0%, 82.8%, and 84.3% nucleotide identity with previously characterized trout IR1, IR3 and IR4, respectively. Quantitative real-time PCR revealed that the four IR mRNAs were differentially expressed, both in terms of distribution among tissues as well as in terms of abundance within selected tissues of juvenile trout. IR1 mRNA was most abundant in spleen, liver, kidney, and muscle (white, red and cardiac), but least abundant in adipose. IR3 mRNA was most abundant in liver, spleen, kidney, and pancreas; in other tissues, levels of IR3 mRNA were uniformly abundant. By contrast, levels of IR2 and IR4 mRNA were uniformly abundant in most tissues, except in spleen where levels of IR4 were significantly lower. All IR subtypes were detected over the course of embryonic development. In head and tail regions, levels of IR2 and IR3 mRNA declined from pre-hatch (29 days post-fertilization, dpf) to post-hatch (68-90 dpf), whereas levels of IR1 and IR4 remained relatively unchanged. These findings contribute to our understanding of the evolution, distribution, and function of insulin receptors. 相似文献
140.
Diane Pourchot M.D. Christine Bodemer M.D. Ph.D. Alice Phan M.D. Ph.D. Anne‐Claire Bursztejn M.D. Ph.D. Smaïl Hadj‐Rabia M.D. Ph.D. Franck Boralevi M.D. Ph.D. Juliette Miquel M.D. Thomas Hubiche M.D. Eve Puzenat M.D. Anne‐Laure Souillet M.D. Ingrid Kupfer M.D. Maryam Piram M.D. Alain Beauchet M.D. Emmanuel Mahé M.D. Groupe de Recherche de la Société Française de Dermatologie Pédiatrique 《Pediatric dermatology》2017,34(1):58-63