An experimental evaluation of traumatic globe rupture

PURPOSE: The purpose of this investigation was to evaluate the surgeon's ability to assess various types of globe injury, to determine the force necessary to rupture the globe with these types of injuries, and to determine typical orbital retraction forces used in the clinical setting. MATERIALS AND METHODS: Forty-four enucleated globes from recently killed cows were divided into four equal groups-one uninjured control group, one group with a through-and-through scleral laceration, another group with a subtotal scleral laceration, and the last group with an 18-gauge needle perforation. Twenty-seven boarded or board eligible oral and maxillofacial surgeons were asked to assess one sample from each of the four groups. They were then asked to retract a simulated globe on a custom-fabricated jig to determine clinical retraction forces. Ten globes from each of the four groups were then subjected to forces until rupture on an Instron 8501M mechanical testing unit. Accuracy of the clinical assessment was determined, and means and standard deviations of the retraction forces and globe rupture forces were derived. RESULTS: Through-and-through lacerations were assessed by surgeons with 100% accuracy, subtotal lacerations with 96% accuracy, uninjured globes with 74% accuracy, and perforated globes with 15% accuracy. Globe rupture occurred at 16.72+/-7.87 kg in the control group, 20.36+/-7.87 kg in the perforated group, 15.38+/-6.06 kg in the subtotal laceration group, and 4.94+/-2.56 kg in the through-and-through laceration group. Statistically significant differences (P < .001) were noted between the total laceration group and all other groups. The mean retraction force was 0.35+/-0.47 kg, which was statistically less than the force used in all of the rupture groups (P < .001). CONCLUSIONS: Severe injuries (through-and-through lacerations) were assessed with 100% accuracy by the clinicians, and less severe injuries with less accuracy. Rupture forces for globes with perforations and subtotal lacerations were no different than for the control group, but substantially less than for the total laceration group. The simulated clinical retraction forces were substantially more than the rupture forces in all of the groups, including the through-and-through laceration group.     

Extensive direct-tandem organization of a long repeat DNA sequence on the Y chromosome of chinook salmon (Oncorhynchus tshawytscha)

A long repetitive DNA sequence (OtY8) has been cloned from male chinook salmon and its genomic organization has been characterized. The repeat has a unit length of 8 kb and is present approximately 300 times per diploid male nucleus. All internal fragments within the 8-kb repeat segregate from father to son, suggesting that the entire repeat unit is located on the Y chromosome. The organization of this sequence into an 8-kb repeat unit is restricted to the Y chromosome, as are several male-specific repeat subtypes identified on the basis of restriction-site variation. The repeat possesses only weak internal sequence similarities, suggesting that OtY8 has not arisen by duplication of a smaller repeat unit, as is the case for other long tandem arrays found in eukaryotes. Based on a laddered pattern arising from partial digestion of genomic DNA with a restriction enzyme which cuts only once per repeat unit, this sequence is not dispersed on the Y chromosome but is organized as a head-to-tail tandem array. Pulse-gel electrophoresis reveals that the direct-tandem repeats are organized into at least six separate clusters containing approximately 12 to 250 copies, comprising some 2.4 Mb of Y-chromosomal DNA in total. Related sequences with nucleotide substitutions and DNA insertions relative to the Y-chromosomal fragment are found elsewhere in the genome but at much lower copy number and, although similar sequences are also found in other salmonid species, the amplification of the repeat into a Y-chromosome-linked tandem array is only observed in chinook salmon. The OtY8 repetitive sequence is genetically tightly associated with the sex-determination locus and provides an opportunity to examine the evolution of the Y chromosome and sex determination process in a lower vertebrate.     

Infant abuse and neglect in monkeys—a discussion of definitions, epidemiology, etiology, and implications for child maltreatment: Reply to Cicchetti (1998) and Mason (1998).

In response to D. Cicchetti's (see record 1998-01884-002) and W. A. Mason's (see record 1998-01884-003) commentaries, this article scrutinizes infant maltreatment in monkeys and its public health implications. Studies of infant abuse and neglect in monkeys have used operational definitions based on (a) adult behavior or (b) adult behavior and infant outcome (depending on data available for analysis). Direct comparisons between the incidence of maltreatment in monkey and human populations can be only tentative as a result of differences in operational definitions of maltreatment. A simplified version of the ecological-transactional model of maltreatment can be used in research with nonhuman primates, and different species can be used to model different aspects of the human phenomenon. Although abuse and neglect take different forms in animals and humans, research with animal models can make an important contribution to elucidating the adaptive function, if any, of child maltreatment and the proximate mechanisms underlying its occurrence. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Genetic manipulation of antibiotic-producing Streptomyces

The genetic manipulation of Streptomyces species has been facilitated by the development of versatile cloning vectors, robust gene transfer systems and transposable elements. These molecular genetic tools have been used to construct antibiotic-producing strains with improved properties and recombinants for the production of hybrid glycopeptide and macrolide antibiotics, novel anthelminthic agents and novel cytotoxic anthracyclines.     

Surface alterations of the bovine oocyte and its investments during and after maturation and fertilization in vitro

Surface characteristics of the bovine oocyte and its investments before, during, and after maturation, and fertilization in vitro were evaluated by scanning electron microscopy (SEM). Oocyte diameters were also measured during SEM analysis of the oocyte. The cumulus cells manifested a compact structure with minimal intercellular spaces among them in the immature oocytes. These became fully expanded with increased intercellular spaces after maturation in vitro, but contracted again after fertilization. The zona pellucida (ZP) showed a fibrous, open mesh-like structure in the maturing and matured oocytes. The size and number of meshes on the ZP decreased dramatically after fertilization. The vitelline surface of immature oocytes was characterized by distribution of tongue-shaped protrusions (TSPs) varying in density. After 10 and 22 hr of maturation incubation, oocyte surface microvilli (MV) increased to become the predominant surface structure, and TSPs decreased substantially. The vitelline surface of fertilized oocytes (at 6 and 20 hr) was similar to that of the matured oocytes, but unfertilized oocytes had less dense MV than did fertilized oocytes (at 20 hr). The diameter of the oocytes decreased from 99 to 80 microns during maturation and increased to 106 microns after insemination (P < 0.05). Membrane maturation was characterized by surface changes from a TSP-predominant pattern to a MV-predominant pattern. Thus, the bovine oocyte maturation process was found to involve the expansion of cumulus cells and the maturation of the ZP, which changes dramatically upon fertilization. Also, volumetric changes occurred in ooplasm processed for SEM following oocyte maturation and insemination.     

2DEG base hot electron transistors fabricated using MBE and in situion beam lithography

A vertical hot electron transistor incorporating a two-dimensional electron gas (2DEG) base has been fabricated in the GaAs-AlGaAs materials system. The difficulties caused by the need to form selective ohmic contacts to the different conducting layers have been overcome using a combination of in situ focused ion beam (FIB) isolation and molecular beam epitaxial (MBE) regrowth. This has allowed a high yield of working devices to be achieved with a typical common emitter current gain of h_FE=6 at low temperatures     

A case of clofazimine enteropathy

A case of clofazimine enteropathy is described. A young male received clofazimine 200 mg daily for four years. He was admitted in a pigmented, emaciated state with abdominal pain, diarrhoea and weight loss. At laparotomy his abdominal organs were stained with dark brown-black pigment due to heavy infiltration with clofazimine crystals. Despite withdrawal of clofazimine his symptoms failed to settle. He developed oedema and hypoalbuminaemia. He died following a cerebral infarction.     

A matrix population model to aid agency response to grass carp (Ctenopharyngodon idella) in the Great Lakes Basin - Lake Erie

Managers and researchers have identified a reproducing population of grass carp (Ctenopharyngodon idella) in the western basin of Lake Erie, generating concern over the potential threat to ecosystem function in the Great Lakes Basin. Capture histories indicate that grass carp may be present at low levels in other areas of Lake Erie, necessitating a large scale, multi-jurisdictional response. As a result, a group of experts and decision makers began a structured decision making exercise to collaboratively address the threat and identify potential response actions. To aid this process, we developed a spatially-explicit periodic matrix population model to project grass carp abundance, and probabilistically evaluate specific management actions. We evaluated four potential management response actions ranging from no action, diffuse removal efforts, and concentrated removal efforts with and without a barrier on the Sandusky River to reduce spawning success. Based on our current knowledge, concentrated removal including a barrier on the Sandusky River provides the most likely path to achieving and maintaining a management target of no more than 10 fish/ha. Our understanding of grass carp ecology in Lake Erie is growing. This model and parameter development methods were designed to flexibly accommodate new information as our understanding of grass carp ecology evolves, or management objectives change. Ultimately, this modeling framework and use of Bayesian methods could facilitate management response efforts for other invasive species occurring over large scales and multiple jurisdictions.     

The controlled rounding problem: Relaxations and complexity issues

Summary Controlled rounding is a procedure that perturbs tabular data collected from respondents in such a way as to preserve the anonymity of the respondents while maintaining the integrity of the data. Controlled rounding techniques are regularly used by the United States Bureau of the Census and its counterparts in other countries. This paper discusses the complexity of the three-dimensional controlled rounding problem. In particular, the three-dimensional, zero-restricted controlled rounding problem is shown to be NP-complete. As zero-restricted controlled roundings may fail to exist, various relaxations of this basic rounding problem have been defined. The paper introduces a sequence of such relaxations and proceeds to address the existence of solutions and complexity issues for the relaxed problems.

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Zusammenfassung Die Technik des kontrollierten Rundens (Controlled Rounding) wird von Behörden wie dem United States Bureau of Census benutzt, um in mehrdimensionalen Tabellen oder Matrizen erfaßte statistische Daten durch Approximation so zu verändern, daß sowohl die Anonymität von Einzeldaten (Matrixeinträgen) als auch die Integrität der Gesamtdaten (Zeilensummen, Spaltensummen etc.) gewährleistet ist. In dieser Arbeit behandeln wir die Komplexität und Lösbarkeit des dreidimensionalen Rundungsproblems und diskutieren eine Hierarchie von Relaxationen, die bei Nichtlösbarkeit des Ausgangsproblems alternative, nahezu zulässige Lösungen liefern.

     

Investigation of fusion reactions in palladium and titanium tritide using galvanostatic,coulometric, and hydrogen permeation techniques

We describe several electrochemical methods used to investigate the possibility of cold fusion phenomena in palladium and titanium tritide cathodes. We performed long-term (up to 77 days) electrolysis experiments with electrochemical cells of the University of Utah type at current densities as high as 1 A/cm², while monitoring neutron and tritium levels. With some cells, we pulsed the current to determine if neutron bursts would result. In another cell, we used titanium tritide as the cathode to determine if D-T reactions yielding neutrons would occur. In no instance were levels of neutrons or tritium significantly above background except in the titanium tritide cell where isotopic exchange, occcurring between the electrode and the electrolyte, resulted in significant tritium levels. We also combined x-ray photoelectron spectroscopy (XPS) and electrochemical hydrogen permeation experiments to determine the effectiveness of various Pd surface treatment procedures on the resultant electrochemical hydrogen absorption efficiency. Electroanalytical and thermal desorption/gas analysis techniques indicated the maximum loading of H in Pd was to a ratio of HPd=0.8.