Susceptibility of heterozygous MnSOD gene-knockout mice to oxygen toxicity

OBJECTIVE: To study type IV collagen of skin and serum in patients with ALS. BACKGROUND: Collagen abnormalities of skin have been reported in ALS patients. However, little is known concerning type IV collagen in ALS. METHODS: We studied type IV collagen immunoreactivity of skin and measured serum levels of the 7S fragment of the N-terminal domain of type IV collagen (7S collagen) in patients with ALS and control subjects. RESULTS: The basement membrane as well as blood vessels of skin in ALS patients was weakly positive for type IV collagen as compared with those of diseased control subjects. This weak immunostaining became more pronounced as ALS progressed. The optical density for type IV collagen immunoreactivity in ALS patients was significantly lower (p < 0.001) than in diseased control subjects and was significantly decreased with duration of illness (r = -0.85, p < 0.01). Serum 7S collagen levels in patients with ALS were significantly decreased (p < 0.01) as compared with those in diseased and healthy control subjects and were negatively and significantly associated with duration of illness (r = -0.81, p < 0.001). There was an appreciable positive correlation between concentrations of serum 7S collagen and the density for type IV collagen immunoreactivity in ALS patients (r = 0.81, p < 0.02). CONCLUSIONS: These data suggest that a metabolic alteration of type IV collagen may take place in the skin of ALS patients and that the decreased levels of serum 7S collagen may reflect a decreased type IV collagen immunoreactivity of skin in patients with ALS.     

Three-phase fluidization: Some knowledge gaps

The most recent review by østergaard — on the fluid mechanics of three-phase fluidized beds — is updated and broadened, and the more manifest knowledge gaps are identified. A systematic classification of the various modes of three-phase fluidization is presented in order to provide some perspective on the research which has been done and on that which has yet to be done.     

Creeping flow relative to permeable spheres

The several possible solutions to the problem of creeping flow relative to an isolated permeable sphere are discussed and compared quantitatively. The most satisfactory solutions is that based upon Brinkman's extension of Darcy's Law. This solution is generalized, using Happel's free-surface model, to cover the practically important problem of flow relative to a swarm of permeable spheres. A physically realistic and mathematically consistent expression for the hydrodynamic resistance experienced by a typical sphere within such a system is derived. This expression is then used to predict the settling rate and the overall permeability of the swarm.