Screening and characterization of microorganisms with glutaryl-7ADCA acylase activity

A screening of microorganisms producing glutaryl-7ADCA acylase, an enzyme able to hydrolyse glutaric acid selectively from glutaryl-3-deacetoxy-7-aminocephalosporanic acid (glutaryl-7ADCA), has been carried out in soil samples. Five microorganisms expressing acylase activity were isolated and classified as Bacillus cereus, Achromobacter xylosooxidans, Bacillus sp., Pseudomonas sp. and Pseudomonas paucimobilis. The screening was carried out by preparing enrichment cultures containing glutaryl-7-ADCA or cephalosporin C as the selective carbon source. Four model compounds (adipoyl-, glutamyl- and glutaryl-p-nitroanilide and glutarylcoumarin), mimicking the glutaryl-7ADCA beta-lactam moiety, were synthesized as substrates suitable for the rapid screening of the microorganisms (2500) isolated from the enrichment cultures. A total of 300 strains were active on the model substrates and only 5 displayed acylase activity on glutaryl-7ADCA. The fermentation parameters, such as pH and inducer concentration, for the optimal acylase expression and acylase specificity towards the model substrates were different for each strain.     

A fast-settling GaAs-enhanced frequency synthesizer

An indirect, phase-locked-loop (PLL), GaAs-enhanced frequency synthesizer with 700 ns loop settling time has been developed. The two-chip GaAs insertion reduced the size of an existing synthesizer from 90 in³ to only 30 in³. The 6.0 in×5.5 in.×0.9 in module contains a 400 gate GaAs programmable divider and a sample and hold (S/H) which improved the settling time by 77% and reduced the size by 67% over the current state-of-the-art synthesizer. Furthermore, the divider reduced power dissipation by 9.7 W and the S/H reduced power dissipation by 1.3 W     

试析加入WTO后我国自动喷水灭火系统设计规范之不足

分析了我国进入 WTO后 ,不容乐观的火灾发展趋势 ,指出了我国现行《自动喷水灭火系统设计规范》存在诸多不足 ,阐明了消防规范和消防管理体制离与国际接轨的路程还很长。列出了 15个问题 ,采用与 NFPA规范和国外认证消防产品技术资料比照的方法 ,来探讨国内规范的不足。     

An electrooptic intensity modulator with improved linearity

A highly linear electrooptic modulator has been designed, fabricated, and evaluated. The design of this modulator consists of only a simple modification to the directional coupler. Two-tone testing has demonstrated that, for an optical modulation depth of 30% per channel, the third-order intermodulation distortion is more than 30 dB lower than that of the conventional directional coupler or Mach-Zehnder modulators. This improvement was not observed to be accompanied by any increase in second harmonic distortion. Also included are results of two-tone computer simulations which predict the improvement in linearity of this device for a range of modulation depths.<>     

Calibration of bistatic polarimetric radar systems

A calibration technique for laboratory type polarimetric, bistatic instrumentation radars is presented. It describes the errors induced by the standard radiation transfer approach (I-SRT) in a way similar to that for the monostatic case. A 12-term error correction and absolute polarimetric calibration is performed with two external reference targets. Only the polarimetric bistatic reference of the first target must be theoretically determined. The scattering reference of the second target is determined by a measurement during the calibration process (single reference calibration). The simulation of a third cross-polarization measurement is performed by an antenna rotation and a remeasurement of the second target. Thus all data are gained for the determination of the error terms and measurements of unknown objects can be full polarimetrically calibrated. The procedures are shown for an adapted dihedral corner reflector. Misalignment errors are discussed     

Decision Support System for Stakeholder Involvement

Stakeholder involvement is essential to the development of a total maximum daily load (TMDL) and its implementation plan. A tool, beyond a simulation model, is needed to support the decision making process that requires negotiation and compromise among stakeholders. The decision support system (DSS) described herein has a TMDL module to calculate various combinations of point and nonpoint loads that can meet the water quality criteria. Its Consensus module allows stakeholders to formulate, evaluate, modify, and vote for alternatives. The DSS displays bar charts for pollution loads from various subwatersheds and attributes the nonpoint loads to land uses. The water quality consequence of the pollution loads is output in maps, which shows sections meeting criteria in green and those not in red. The DSS requires a front end effort of site specific adaptation and model calibration. An Internet-based stakeholder process was developed to allow more concerned citizens to participate in management decisions.     

IEEE 802.11 roaming and authentication in wireless LAN/cellular mobile networks

A wireless LAN service integration architecture based on current wireless LAN hot spots is proposed so that migration to a new service becomes easier and cost effective. The proposed architecture offers wireless LAN seamless roaming in wireless LAN/cellular mobile networks. In addition, a link-layer-assisted mobile IP handoff mechanism is introduced to improve the network/domain switching quality in terms of handoff delay and packet loss. An application layer end-to-end authentication and key negotiation scheme is proposed to overcome the open-air connection problem existing in wireless LAN deployment. The scheme provides a general solution for Internet applications running on a mobile station under various authentication scenarios and keeps the communications private to other wireless LAN users and foreign network. A functional demonstration of the scheme is given. The research results can contribute to rapid deployment of wireless LANs.     

Regulatorische Aspekte der dezentralen Erzeugung

Ohne Zusammenfassung Kurzfassung eines Vortrags der 41. Fachtagung der ?sterreichischen Gesellschaft für Energietechnik (OGE) im OVE, die am 5. und 6. November 2003 in Salzburg stattfindet.     

主动约束层阻尼板结构动力学建模研究

将有限元法和GHM法相结合，给出了建立主动约束层阻尼（ACLD）板结构动力学模型的新方法。建模时，考虑到粘弹材料（VEM）的纵向位移影响，采用GHM方法描述VEM的本构关系，解决了VEM的力学特性随温度和频率变化的难点，避免因VEM的复杂本构关系而产生的微分积分方程。算例表明本文给出的建模方法是准确的，ACLD结构能够有效控制结构振动。     

Alliance and Dropout in Family Therapy for Adolescents With Behavior Problems: Individual and Systemic Effects.

This study examined the relationship between alliance and retention in family therapy. Alliance was examined at the individual (parent and adolescent) and family levels (within-family differences). Participants were 34 families who received functional family therapy for the treatment of adolescent (aged 12-18 years) behavior problems. Families were classified as treatment dropouts (n=14) or completers (n=20). Videotapes of the first sessions were rated to identify parent and adolescent alliances with the therapist. Results demonstrated that individual parent and adolescent alliances did not predict retention. However, as hypothesized, dropout cases had significantly higher unbalanced alliances (parent minus adolescent) than did completer cases. These findings highlight the importance of alliances in functional family therapy and suggest that how the alliance operates in conjoint family therapy may be a function of systemic rather than of individual processes. (PsycINFO Database Record (c) 2010 APA, all rights reserved)