Two-step purification strategy for enhanced recovery of recombinant hepatitis B surface antigen from Pichia pastoris

Univariate screening on factors affecting the purification performance of recombinant hepatitis B surface antigen (HBsAg) on ion exchange chromatography (IEC) and size exclusion chromatography (SEC) and the establishment of a two-step purification strategy were performed. Amongst four IEC adsorbents examined, the use of Q Sepharose XL IEC adsorbent under optimized conditions together with optimized SEC purification was able to efficiently purify HBsAg. An established purification strategy comprising the two techniques further demonstrated adaptability for scale-up operations with a final total purification factor (PF) of 94.82 ± 16.20, HBsAg purity of 95.48% and recovery yield of 78.07%.     

Exploring the Biosynthesized Metal Nanoparticles for their Catalytic Degradation of Toxic Water Wastes and Antimicrobial Potential

Journal of Inorganic and Organometallic Polymers and Materials - In recent decades, the analysis of nanoparticles is of greater importance for their applications in various fields. This present...     

Tocotrienol Rich Fraction Reverses Age-Related Deficits in Spatial Learning and Memory in Aged Rats

Little is known about the effect of vitamin E on brain function. Therefore, in this study we evaluated the effect of tocotrienol rich fraction (TRF) on behavioral impairment and oxidative stress in aged rats. Thirty-six male Wistar rats (young: 3-months-old; aged: 21-months-old) were treated with either the control (olive oil) or TRF (200 mg/kg) for 3 months. Behavioral studies were performed using the open field test and Morris water maze (MWM) task. Blood was taken for assessment of DNA damage, plasma malondialdehyde (MDA) and vitamin E, and erythrocyte antioxidant enzyme activity. Brains were also collected to measure vitamin E levels. Results showed that aged rats exhibited reduced exploratory activity, enhanced anxiety and decreased spatial learning and memory compared with young rats. DNA damage and plasma MDA were increased, and vitamin E levels in plasma and brain were reduced in aged rats. Aged rats supplemented with TRF showed a markedly reduced level of anxiety, improved spatial learning and memory, reduced amount and severity of DNA damage, a reduced level of MDA, and increased levels of antioxidant enzyme activity and plasma/brain vitamin E compared with age-matched controls. In conclusion, TRF supplementation reverses spatial learning and memory decline and decreases oxidative stress in aged rats.     

Human mismatches and preferences for automation

The research reported in this paper is concerned with gaining a better understanding of human factors issues in machining and the automation of manufacturing tasks. Mismatches between operators' performance and the requirements of machining tasks were experimentally studied with respect to the relationships with various human characteristics, including skill, age, work experience, self-confidence and trust. Twelve hypotheses concerning interrelationships between these characteristics were evaluated and important relationships established. It is considered that this increased knowledge of the rate of mismatches and an understanding of the causes is essential for the successful design of new working environments, machines and tasks. Much of this change to the working environment is likely to involve some degree of automation of the operators' tasks and so a second and important aspect of the study was designed to establish the extent to which preferred levels of automation were related to the same human characteristics. Four further hypotheses relating preferred levels of automation to skill, age, work experience, self-confidence and trust were tested with results that, in some cases, were unexpected and in others contradict the findings of previous research.     

Application of electromagnetic impact technique for welding copper-to-stainless steel sheets

The ability to manufacture a product using different metal combinations greatly increases flexibility in design and production. Joining of dissimilar metal combinations like Copper-to-Stainless Steel (Cu-to-SS) is, however, a challenging task owing to the large differences in physical and chemical properties. The application of electromagnetic (EM) impact technique is demonstrated for welding copper (Cu) to stainless steel (SS) sheets. The welding Cu-to-SS is accomplished by using Al drivers to accelerate Cu and SS work sheets. The tensile shear strength test and the metallographic studies are carried out for Cu-to-SS EM welds.     

Studies on the growth and stability of silver nanoparticles synthesized by electron beam irradiation

Stable Ag nanoparticles have been synthesized by irradiating an aqueous solution of AgNO₃ and Poly-vinyl alcohol (PVA) with 8 MeV electrons from a Microtron. The rate of formation of nanoparticles could be controlled by changing either the irradiation dosage or the relative concentration of the precursors. The size, shape, and the rate of formation of the nanoparticles depend on the final dosage, as well as the weight ratio of AgNO₃ and PVA. The formation of Ag nanoparticles and their size were established through UV–Vis spectroscopy and Transmission electron microscopy (TEM) analysis, respectively. Increasing the irradiation dosage seem to favour the formation of polygonal nanostructures. Differential scanning calorimetry (DSC) measurements show that there exists a strong interaction between the PVA matrix and the Ag nanoparticles.     

Dielectric relaxation and conduction mechanism of complex perovskite Ca0.90Sr0.10Cu3Ti3.95Zn0.05O12 ceramic

In this work, Sr and Zn co-doped calcium copper titanate having nominal formula Ca_0.90Sr_0.10Cu₃Ti_3.95Zn_0.05O₁₂ has been synthesized and systematically studied for structure, dielectric and electrical properties. The phase purity of the prepared composition has been confirmed by X-ray diffraction (XRD) pattern and various structural parameters are presented here with Rietveld refinement data. The Scanning electron microscopy (SEM) image revealed the presence of homogeneous fine-grained microstructure investigated in the prepared ceramic. The impedance data well fitted with 2 R-CPE model confirmed the non-ideality of the system. Dielectric studies have shown the existence of two relaxation processes in low and high frequency domains. Low frequency dielectric response proposed as a combination of Maxwell-Wagner relaxation and intrinsic defect (V_o⁺⁺) while high frequency behavior has been interpreted in terms of polaronic relaxation caused by hopping process of an electron between Ti³⁺ and Ti⁴⁺ states. The frequency dependent behavior of conductivity has been well fitted to Dong’s model and interpreted in term of Overlapping Large Polaron Tunneling (OLPT) mechanism. The various parameters; relaxation angular frequency (ω₁) of Debye process, ionic hopping frequency (ω₂₎, dc conductivity (σ_o), cole- cole parameter (α) and frequency exponent (s) have been determined by Dong’s Model fitting.     

Complex methylene-blue-sensitized polyvinyl chloride: a polymer matrix for hologram recording

A new polymer matrix sensitized with methylene blue for use as an optical recording material is described here. The characterization is done to determine the optimal recording conditions. These films need no chemical development and are found to be stable for several months. The matrix has excellent shelf life and needs an exposure only as short as 20 s. Direct imaging was done on this material.     

Realization of a compact microstrip antenna: An optimization approach

A new compact square microstrip patch antenna is proposed using the optimization approach, namely, the genetic algorithm (GA). The antenna is designed at three different frequencies, 785 MHz., 1.57 GHz, and 2.46 GHz, for mobile communication, GPS, and Bluetooth applications, respectively. The simulations are carried out using IE3D from Zeland Software, which is based on the method of moments (MoM). The antenna consists of a probe‐fed truncated‐corner square patch with four inserted slits along the four diagonals and four angular grooves along the four edges of the patch. The proposed design has a reduced antenna size, as compared to the conventional microstrip antenna at a given operating frequency. The return loss, impedance, and axial‐ratio bandwidth are determined and compared with the conventional square‐patch antenna. © 2006 Wiley Periodicals, Inc. Int J RF and Microwave CAE, 2006.