Easily constructed spectroelectrochemical cell for batch and flow injection analyses

The design and performance of an easily constructed spectroelectrochemical cell suitable for batch and flow injection measurements are described. The cell is fabricated from a commercially available 5-mm quartz cuvette and employs 60 ppi reticulated vitreous carbon as the working electrode, resulting in a reasonable compromise between optical sensitivity and thin-layer electrochemical behavior. The spectroelectrochemical traits of the cell in both batch and flow modes were evaluated using aqueous ferricyanide and compare favorably to those reported previously for similar cells.     

Multiple ion isolation applications in FT-ICR MS: exact-mass MSn internal calibration and purification/interrogation of protein-drug complexes

Two new applications using multiple ion isolations in the cell of a Fourier transform-ion cyclotron resonance mass spectrometer equipped with an electrospray ionization source are described. A procedure that uses multiple ion isolations of an analyte and calibrants for internal calibration at each stage in a MSn experiment, under high-resolution exact-mass conditions, for structural characterization/elucidation of angiotensin I and rapamycin is illustrated. Fragment ion mass accuracies < 1.0 ppm are demonstrated and routinely achieved. Purification of a mixture is illustrated by isolating multiple charge states of a protein-drug complex from residual protein for further MSn studies to elucidate the site of covalent drug bonding using IRMPD for a mixture of epidermal growth factor receptor (EGFr) protein and EGFr-drug complex. The procedure developed for multiple ion isolations is referred to as multi-CHEF, multiple correlated harmonic excitation fields, in which tailored waveforms are used to notch out multiple mass regions of a spectrum with minimal off-resonance excitation.     

Simulation and optimization of retention in ion chromatography using virtual column 2 software

A new software package, Virtual Column 2, is described for the simulation and optimization of the separation of inorganic anions by ion chromatography (IC). The software uses a limited amount of experimental retention data acquired according to a correct experimental design to predict retention times for analytes over a designated search area of eluent compositions. The experimental retention data are used to solve a new retention model, called the linear solvent strength model, empirical approach (LSSM-EA), which then enables prediction of retention times for all eluent compositions in the search area. The theoretical development of LSSM-EA and the processes used for solving the equations are discussed. Virtual Column 2 can be used for eluents containing one or two competing ions, and the software contains retention databases for up to 33 analytes on the Dionex AS9A-HC, AS4A-SC, and AS14A analytical columns with carbonate-bicarbonate eluents and the Dionex AS10, AS15, and AS16 analytical columns with hydroxide eluents (results for the AS10 and AS15 columns are not discussed in the present study). Virtual Column 2 has been evaluated extensively and is shown to give predicted retention times that in most cases agree with experimentally determined data to within 5%. The software has uses in practical IC method development, education and training in IC, and refinement of existing IC methodology. A free version of this program is available by download at www.virtualcolumn.com.     

Neuronal morphology data bases: morphological noise and assesment of data quality

For technical, instrumental and operator-related reasons, three-dimensional reconstructions of neurons obtained from intracellularly stained neuronal pieces scattered in serial sections are blurred by some morphological noise. This noise may strongly invalidate conclusions drawn from models built using the three-dimensional reconstructions and it must be taken into account when retrieving digitized neurons from available databases. We analyse the main generating sources of the noise and its consequences for the 'quality' of the data. We provide tools for detecting and evaluating the noise in any database providing sufficient information is given in the database. We propose a unified format for submitting data and a new neuron viewer/editor to analyse the digitized neurons with our tools.     

Author Index,Volume 8 (2002)

Authors Index

Author Index, Volume 8 (2002)     

Past, Present, and Future

Thomas L. Friedman. the World Is Flat: A Brief History of the Twenty-First Century. N ew York: Farrar, Straus and Giroux, 2005, 488 pp.

Nicholas G. Carr. Does IT Matter? Information Technology and the Corrosion of Competitive Advantage. Boston: Harvard Business School Press, 2004, 193 pp.

John Hagel III and John Seely Brown. the Only Sustainable Edge: Why Business Strategy Depends on Productive Friction and Dynamic Specialization. Boston: Harvard Business School Press, 2005, 218 pp.

John Markoff. What the Dormouse Said. New York: Viking, 2005, 301 pp.     

Virtual human animation in natural language visualisation

Simulation motion of Virtual Reality (VR) objects and humans has experienced important developments in the last decade. However, realistic virtual human animation generation remains a major challenge, even if applications are numerous, from VR games to medical training. This paper proposes different methods for animating virtual humans, including blending simultaneous animations of various temporal relations with multiple animation channels, minimal visemes for lip synchronisation, and space sites of virtual human and 3D object models for object grasping and manipulation. We present our work in our natural language visualisation (animation) system, CONFUCIUS, and describe how the proposed approaches are employed in CONFUCIUS’ animation engine.     

Temperature-dependent microtensile testing of thin film materials for application to microelectromechanical system

A specially designed microtensile apparatus capable of carrying out a series of tests on microscale thin films for microelectromechanical system (MEMS) applications at room temperature and at temperature up to 400°C has been developed and tested, and is described here. Several MEMS-applicable thin films were measured with it, including thermally grown silicon dioxide, gold, and gold–vanadium. The silicon dioxide was tested at room temperature. Gold and gold–vanadium films were tested at room temperature and at 200 and 400°C. Examples of these results are presented.

     

Transient experiments from the thermal-hydraulic ADS lead bismuth loop (TALL) and comparative TRAC/AAA analysis

TALL is a medium-size experimental facility constructed at KTH, to study the steady-state and transient thermal-hydraulics performance of LBE-cooled reactors, with the primary purpose of supporting the European transmutation demonstration (ETD) using LBE-cooled accelerator-driven systems (ADS). This paper presents the results of transient experiments performed on the TALL test facility, whose aim is to provide a data base for validation of computer codes which may be used for the analysis of the safety of those systems. This paper also presents the results of the post-test calculations, carried out at PSI, using the TRAC/AAA code. The transient experiments performed include the loss of heat sink, the loss of pump, the loss of both primary and secondary flows, overpower, overcooling, heater trip, and the operational transients of start-up and shut-down. The experimental results show the excellent natural circulation performance of a LBE-cooled system which should contribute to a good safety performance. The TRAC/AAA calculations provide results which agree well with the experimental data.