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Rafaelle Spear Ludovic Boytard Renaud Blervaque Maggy Chwastyniak David Hot Jonathan Vanhoutte Bart Staels Yves Lemoine Nicolas Lamblin Fran?ois-René Pruvot Stephan Haulon Philippe Amouyel Florence Pinet 《International journal of molecular sciences》2015,16(5):11276-11293
Abdominal aortic aneurysm (AAA) is an inflammatory disease associated with marked changes in the cellular composition of the aortic wall. This study aims to identify microRNA (miRNA) expression in aneurysmal inflammatory cells isolated by laser microdissection from human tissue samples. The distribution of inflammatory cells (neutrophils, B and T lymphocytes, mast cells) was evaluated in human AAA biopsies. We observed in half of the samples that adventitial tertiary lymphoid organs (ATLOs) with a thickness from 0.5 to 2 mm were located exclusively in the adventitia. Out of the 850 miRNA that were screened by microarray in isolated ATLOs (n = 2), 164 miRNAs were detected in ATLOs. The three miRNAs (miR-15a-3p, miR-30a-5p and miR-489-3p) with the highest expression levels were chosen and their expression quantified by RT-PCR in isolated ATLOs (n = 4), M1 (n = 2) and M2 macrophages (n = 2) and entire aneurysmal biopsies (n = 3). Except for the miR-30a-5p, a similar modulation was found in ATLOs and the two subtypes of macrophages. The modulated miRNAs were then evaluated in the plasma of AAA patients for their potential as AAA biomarkers. Our data emphasize the potential of miR-15a-3p and miR-30a-5p as biomarkers of AAA but also as triggers of ATLO evolution. Further investigations will be required to evaluate their targets in order to better understand AAA pathophysiology. 相似文献
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Scientometrics - This study investigates a potential relationship between highly-cited scholarly papers and the number of citations received by other papers with which they share a journal issue.... 相似文献
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Metallurgist - We consider domestic and foreign standards for rolled metals used in bridge building. Domestic standards contain elevated requirements to the reliability of rolled metals in terms of... 相似文献
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Kolmachikhina E. B. Sviridov A.V. Naumov K. D. 《Russian Journal of Non-Ferrous Metals》2020,61(5):488-493
Russian Journal of Non-Ferrous Metals - This article is devoted to the influence of sodium lignosulfonate (SL), anionic surfactants (sodium dodecylsulfate (SDS), sodium dodecylbenzene sulfonate... 相似文献
69.
Grigor’eva N. G. Serebrennikov D. V. Bubennov S. V. Kutepov B. I. 《Catalysis in Industry》2020,12(1):47-55
Catalysis in Industry - The oligomerization of 1-pentene is an efficient process for producing environmentally friendly high-quality fuel components. The catalytic properties of FAU, OFF, MOR, BEA,... 相似文献
70.
Amlabu B. A. Umaru S. Dauda M. Obada D. O. Csaki S. Bansod N. D. Dodoo-Arhin D. Fasanya O. O. 《SILICON》2020,12(6):1311-1324
Silicon - In this study, an evaluation has been made into the influence of milling time on the thermo-physical and fracture properties of a MgO-Al2O3-SiO2 system. The powdery and compacted... 相似文献