Mapping of continuous floristic gradients in grasslands using hyperspectral imagery

Transitions between plant species assemblages are often continuous with the form of the transition dependent on the ‘slope’ of environmental gradients and on the style of self-organization in vegetation. Image segmentation can present misleading or even erroneous results if applied to continuous spatial changes in vegetation. Even methods that allow for multiple-class memberships of pixels presuppose the existence of ideal types of species assemblages that constitute mixtures—an assumption that does not fit the case of continua where any section of a gradient is as ‘pure’ as any other section like in modulations of grassland species composition.Thus, we attempted to spatially model floristic gradients in Bavarian meadows by extrapolating axes of an unconstrained ordination of species data. The models were based on high-resolution hyperspectral airborne imagery. We further modelled the distribution of plant functional response types (Ellenberg indicator values) and the cover values of selected species. The models were made with partial least squares (PLS) regression analyses. The realistic utility of the regression models was evaluated by full leave-one-out cross-validation.The modelled floristic gradients showed a considerable agreement with ground-based observations of floristic gradients (R²=0.71 and 0.66 for the first two axes of ordination). Apart from mapping the most important continuous floristic differences, we mapped gradients in the appearance of plant functional response groups as represented by averaged Ellenberg indicator values for soil pH (R²=0.76), water supply (R²=0.66) and nutrient supply (R²=0.75), while models for the cover of single species were weak.Compared to many other vegetation attributes, plant species composition is difficult to detect with remote sensing techniques. This is partly caused by a lack of compatibility between methods of vegetation ecology and remote sensing. We believe that the present study has the potential to increase compatibility as neither spectral nor vegetation information gets lost by a classifying step.     

Performing Aboriginality: Desiring Pre-contact Aboriginality in Victoria, 1886–1901

This paper re-investigates notions of performed Aboriginality in relation to photographs made at Lake Tyers Mission Station, Victoria, Australia, and argues that Nicholas Caire's photographs reveal complex Aboriginal subjectivities. The photographs, made originally in 1886 and distributed to tourists, were later reproduced and circulated in book format in 1897. The first presentation of the photographs, whilst focusing on historical Aboriginality, contains traces of cross-cultural hybridity. However, the later presentation of the work reinforces historical and traditional material culture over cross-cultural dialogue. This paper argues that the desire to find historical notions of Aboriginality on mission stations in Victoria was not just due to the establishment of hierarchical racial theories in the latter part of the nineteenth century (generating the idea that Aborigines could not change and adapt to notions of ‘civilisation’) and doubts about the success of mission stations, but also because there was an interest in Aborigines who had experienced little assimilation from more remote parts of the continent of Australia. This curiosity in pre-contact Aboriginality fuelled tourism in Victoria to accessible mission stations such as Lake Tyers.     

Vibrant Photography: Photographs,actants and political ecology

Jane Bennett’s thesis in Vibrant Matter argues that we need to find ways to acknowledge and engage with the agency of all factors that create ecologies and environments, including non-human actants. This essay argues that Richard Misrach and Kate Orff’s project Petrochemical America signals some important strategies that are useful for developing visual political ecologies and understanding non-human actants, including the non-living. Although some of the images are typical scenes of environmental devastation, human and social links are made to foster the understanding that we are looking at an image of ecology rather than nature ravaged. In addition to the titles of images, captions and overall contextualization of the project, Kate Orff’s team SCAPE produce a series of diagrams called Throughlines that situate the subject of petrochemical creation and its impact in the Mississippi River corridor. The cumulative impact of Misrach’s photographic approach, combined with the effective and systematic linking of his images to other forms of relevant information by Kate Orff, produces an understanding of environment as not only inextricably linked to human activity and habitation, but which also contains differing agencies constituting a broader ecology.     

Integrative Analysis of Metabolomic,Proteomic and Genomic Data to Reveal Functional Pathways and Candidate Genes for Drip Loss in Pigs

The aim of this study was to integrate multi omics data to characterize underlying functional pathways and candidate genes for drip loss in pigs. The consideration of different omics levels allows elucidating the black box of phenotype expression. Metabolite and protein profiling was applied in Musculus longissimus dorsi samples of 97 Duroc × Pietrain pigs. In total, 126 and 35 annotated metabolites and proteins were quantified, respectively. In addition, all animals were genotyped with the porcine 60 k Illumina beadchip. An enrichment analysis resulted in 10 pathways, amongst others, sphingolipid metabolism and glycolysis/gluconeogenesis, with significant influence on drip loss. Drip loss and 22 metabolic components were analyzed as intermediate phenotypes within a genome-wide association study (GWAS). We detected significantly associated genetic markers and candidate genes for drip loss and for most of the metabolic components. On chromosome 18, a region with promising candidate genes was identified based on SNPs associated with drip loss, the protein “phosphoglycerate mutase 2” and the metabolite glycine. We hypothesize that association studies based on intermediate phenotypes are able to provide comprehensive insights in the genetic variation of genes directly involved in the metabolism of performance traits. In this way, the analyses contribute to identify reliable candidate genes.     

Effect of pretreatment temperature on the surface modification of diatomite with trimethylchlorosilane

Diatomite samples from Costa Rica were purified using acidic treatments with hydrochloric acid, thermally treated (400–1000 °C) and then silylated with trimethylchlorosilane in toluene under inert atmosphere. The purification process allows to decrease the concentration of metals presented in the crude diatomite, as is confirmed by X-ray Fluorescence (XRF) Analysis. The silylated materials were analyzed by using Hyperpolarized ¹²⁹Xe Nuclear Magnetic Resonance Spectroscopy (HP ¹²⁹Xe NMR), Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Thermogravimetric Analysis (TGA), rehydration tests, and contact angle measurements. XRD measurements indicate that diatomite is mainly amorphous, but presents several crystalline phases (kaolinite, cristobalite, and quartz). Pretreatments at high temperatures cause changes in those crystalline phases, resulting in more amorphous materials. However, there is no difference in the overall structure of purified and thermally treated diatomite samples with respect to the silylation products. In addition, SEM measurements show no effect over the pore structure of the materials. On the other hand, TGA measurements and rehydration tests show lower losses of water for silylated materials prepared using higher pretreatment temperatures. Moreover, HP ¹²⁹Xe NMR, FTIR, and contact angle measurements evidence a modification due to covalent attachment of Si(CH₃)₃-groups to the surface, which increases for higher pretreatment temperatures. The results provide valuable information about external factors that influence the surface modification of diatomite. This can be useful to control modifications that can be achieved in a similar way.     

Microwave-assisted synthesis and characterization of bioactive chitosan scaffolds doped with Au nanoparticles for mesenchymal stem cells culture

In this work we present a novel strategy for chitosan-based scaffolds. Chitosan is a versatile biopolymer obtained from waste biomass known of its favorable biological properties. Thus it can replace other polymers in the preparation of bioactive scaffolds. To increase its durability chitosan can be crosslinked into form of the hydrogel yet application of toxic crosslinkers may lead to loss of biocompability. Mesenchymal stem cells can be used in cell therapy for advanced wound treatment. However their culture requires special biomaterials application. In this article a novel microwave-assisted synthesis method for bioactive chitosan scaffolds is presented.     

Screening of different agroindustrial by-products for industrial enzymes production by fermentation processes

Agroindustrial by-products are an abundant source of biocompounds that contain valuable nutrients, which are not exploited. In this work, lignocellulosic wastes (LW) were used in submerged fermentation (SmF) and solid-state fermentation (SSF) by Aspergillus niger NRRL3 to obtain valuable enzymes required in industries. SmF using soya bean hulls (SH), wheat bran (WB) and a by-product of wheat flour (F) produced the highest activities of endo-1,4-β-xylanase (Xyl) and endo-1,4-β-D-glucanase (EG) being at least 3 times lower than those obtained by SSF. The highest ratio of Xyl to EG was obtained in SmF with F. Xyl obtained by SmF with WB was the most thermally resistant. The enzymatic extract obtained in SmF using SH presented a high power of saccharification. The production of enzymes for further application such as bioethanol generation process revalue these LW and can help offset growing environmental problems.