Misidentification of Vibrio cholerae O155 isolated from imported shrimp as O serogroup O139 due to cross-agglutination with commercial O139 antisera

Fish and shellfish products imported into Denmark are routinely analyzed for pathogenic Vibrio spp., particularly Vibrio cholerae, if products originate from subtropical or tropical areas. A V. cholerae strain that agglutinated commercial O139 antiserum but not the O1, Inaba, or Ogawa antisera was isolated from imported raw frozen shrimp. The toxigenicity of the strain was analyzed, and the results of a polymerase chain reaction showed that the V. cholerae strain did not contain the virulence genes ctx, tcpA, and zot, which are normally found in V. cholerae O1 and O139. The strain was resistant to colistin and spectinomycin. The high susceptibility of the strain to antimicrobial agents was confirmed by the lack of an SXT element, a self-transmissible, chromosomal genetic element that is normally present in 0139 strains and encodes resistance to sulfonamides, trimethoprim, and streptomycin. The strain contained two plasmids, in contrast to other O139 strains, which normally do not contain plasmids. The characteristics of the strain led to further agglutination testing with other antisera that are not commercially available, and the strain was found to agglutinate O155 antiserum in repeated testing. Manufacturers of 0139 antiserum should be aware of the closely related O antigens of the O139, O22, and O155 serogroups and should be aware that their commercial diagnostic O139 antiserum must be absorbed to remove cross-reacting agglutinins of O22 and O155 strains.     

Effect of spacer arm length on protein retention on a strong cation exchange adsorbent

The retention of five proteins was compared on a set of three strong cation exchange adsorbents that differed in spacer arm chemical structure and length. The adsorbents included a commercial product, Amersham Biosciences SP Sepharose Fast Flow, containing a six-carbon spacer between the agarose matrix and the anionic ligand, and two custom-prepared materials. One of the custom adsorbents contained a spacer of about half the length of the SP Sepharose Fast Flow, and the other contained no spacer arm. The adsorbent with no spacer arm was found to be significantly more retentive for all of the test proteins examined, in both isocratic and gradient elution tests. Reducing the spacer arm length by half resulted in increased retention for four of the five proteins, but this increase was less than what was observed when the spacer arm was eliminated. Retention increases were obtained without increasing the density of the anionic charge groups and appear to result from an enhancement of electrostatic or secondary nonelectrostatic interactions, or both. The results indicate that spacer arm length may be a useful variable in manipulating stationary-phase retention properties.     

Binary monolayers of single-stranded oligonucleotides and blocking agent for hybridisation

The hybridisation of thiol-modified single-stranded DNA (SH-ssDNA) embedded in lipoate or hydrophilic polymer monolayers on gold have been studied through the technique of surface plasmon resonance (SPR). SH-ssDNA and the blocking agents were co-adsorbed on gold from the same solution or the surface was post-treated with the blocking agents. The assembling process is allowed to take place for only 10 min. Binding of non-complementary and complementary DNA is dependent both on the blocking agent and on the assembling method used. The lowest non-complementary binding and the highest complementary binding of DNA was obtained with a layer assembled from a binary solution of SH-ssDNA and the lipoate blocking agent.     

Late psychosocial improvement in a case of severe head injury with bilateral fronto-orbital lesions.

Describes the results of a 19-yr follow-up study of a head-injured female patient. At age 17 yrs, the S sustained a blunt head trauma with bilateral fronto-orbital lesions verified by late regional cerebral blood flow. The S presented an extreme state of regression for many months after injury. During the first posttraumatic years no behavioral disorders were reported, but after the 5th yr lack of control, including sexual disinhibition, and lack of motivation became severe problems. More than 10 yrs after the accident, a partner applied a simple program of behavioral modification to the S. After 2 yrs of rigid training, an unexpected improvement in psychosocial adaptation took place. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Comparative emission analysis of low-energy and zero-emission buildings

Different designs and concepts of low-energy and zero-emission buildings (ZEBs) are being introduced into the Norwegian market. This study analyses and compares the life cycle emissions of CO₂ equivalents (CO₂e) from eight different single-family houses in the Oslo climate. Included are four ZEBs: one active house, two passive houses, and a reference house (Norwegian building code of 2010). Monthly differences in CO₂e emissions are calculated for the seasonally sensitive Norwegian context for electricity generation and consumption. This is used to supplant the previous applied symmetric weighting approach for CO₂e/kWh factors for import and export of electricity for the ZEB cases. All the ZEBs have lower use-stage emissions compared with the other buildings or the reference case. Embodied impacts are found to be 60–75% for the analysed ZEB cases, confirming the importance of embodied impacts in Norwegian ZEBs. The lowest total emissions were from the smallest ZEB, emphasizing area efficiency. The highest emissions were from the reference case. By abandoning the symmetric approach, a new perspective was developed for assessing the performance of ZEBs within the Norwegian context. One of four ZEB cases managed to balance out its annual energy-related emissions.     

Effects of cereal breakfasts on postprandial glucose,appetite regulation and voluntary energy intake at a subsequent standardized lunch; focusing on rye products

Background  

Rye products have been demonstrated to lower the acute insulin demand, induce a low and prolonged blood glucose response (high Glycemic Profile, GP) and reduce subclinical inflammation. These products may therefore contribute to a lowered risk of obesity, type 2 diabetes and cardio vascular disease. The objective of the present paper was to evaluate the mechanism for a reduced postprandial insulin demand with rye products, and to explore possible appetite regulating properties.     

The impact of a call centre on communication in a programme and its projects

Call centres are increasingly being utilised in public sector programmes to facilitate and manage communication between numerous stakeholders. Yet, the impact of call centres on projects has not been investigated. This paper reports on a survey with 92 respondents that assessed the impact of a call centre for a repair and maintenance programme. An empirically verified model is presented to illustrate the relationship between call centre communication and project performance. A balance of frequent informal and formal communication is shown to reduce mistrust and conflict of interest resulting from each party trying to maximise his respective economic position in the principal–agency relationship. The data provides evidence that a call centre improves the communication, collaboration and trust in project principal–agency relationships which, in turn, is perceived to contribute to project performance.     

Evaluation of energy and dietary intake estimates from a food frequency questionnaire using independent energy expenditure measurement and weighed food records

Background  

We have developed a food frequency questionnaire (FFQ) for the assessment of habitual diet, with special focus on the intake of fruit, vegetables and other antioxidant-rich foods and beverages. The aim of the present study was to evaluate the relative validity of the intakes of energy, food and nutrients from the FFQ.     

Urban infrastructure: four perspectives on Swedish development

From an international perspective, Sweden enjoys a leading position concerning urban infrastructure as the industrialized world is struggling with similar problems. In many countries, the situation is critical. By taking advantage of the short channels of communication between the central and the local governmental bodies, researchers and industry in Sweden can develop knowledge of interest to the entire international community. This feature article combines four essays by leading Swedish experts in the field of infrastructure development.     

Nonpharmacological interventions for insomnia in older adults: A meta-analysis of treatment efficacy.

The benefit of nonpharmacological interventions for insomnia in old age was investigated. A total of 13 single-outcome studies from 1966–1998 involving 388 patients (mean age exceeding 60 years, minimum age in sample, 50 years) were included in a meta-analysis of treatment efficacy. This analysis demonstrated that behavioral interventions produce improvements in sleep parameters of older insomniacs, measured in terms of sleep-onset latency, number of nocturnal awakenings, time awake after sleep onset, and total sleep time. Clinical improvements seen at posttreatment were maintained at followups (averaging 6 months). It is concluded that behavioral treatments produce significant and long-lasting improvements in the sleep pattern of older insomniacs. (PsycINFO Database Record (c) 2010 APA, all rights reserved)