全文获取类型
收费全文 | 48410篇 |
免费 | 2185篇 |
国内免费 | 3503篇 |
学科分类
医药卫生 | 54098篇 |
出版年
2024年 | 104篇 |
2023年 | 452篇 |
2022年 | 202篇 |
2021年 | 248篇 |
2020年 | 153篇 |
2019年 | 124篇 |
2018年 | 153篇 |
2017年 | 81篇 |
2016年 | 140篇 |
2015年 | 201篇 |
2014年 | 481篇 |
2013年 | 226篇 |
2012年 | 8448篇 |
2011年 | 9134篇 |
2010年 | 1575篇 |
2009年 | 668篇 |
2008年 | 4966篇 |
2007年 | 4981篇 |
2006年 | 4492篇 |
2005年 | 3962篇 |
2004年 | 3303篇 |
2003年 | 2929篇 |
2002年 | 2213篇 |
2001年 | 1321篇 |
2000年 | 1699篇 |
1999年 | 855篇 |
1998年 | 304篇 |
1997年 | 81篇 |
1996年 | 56篇 |
1995年 | 61篇 |
1994年 | 37篇 |
1993年 | 33篇 |
1992年 | 17篇 |
1991年 | 8篇 |
1990年 | 8篇 |
1989年 | 44篇 |
1988年 | 33篇 |
1987年 | 25篇 |
1986年 | 16篇 |
1985年 | 20篇 |
1984年 | 31篇 |
1983年 | 25篇 |
1982年 | 20篇 |
1981年 | 18篇 |
1980年 | 23篇 |
1979年 | 14篇 |
1965年 | 13篇 |
1964年 | 11篇 |
1963年 | 9篇 |
1962年 | 10篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
21.
Sun Weidong Hansell Stedman Chen Xiaoli Wei Yu Liu Meizhen Xu Chengbin H. Lee Sweeney 《中华医学杂志(英文版)》1998,(6):495
LigasechainreactionfordiagnosisoffamilialhypertrophiccardiomyopathySunWeidong孙维东,HanselStedman,ChenXiaoli,WeiYu,LiuMeizhen,Xu... 相似文献
22.
Good regulation of acupuncture in simple obesity patients with stomach-intestine excessive heat type
下载免费PDF全文
![点击此处可从《中国结合医学杂志》网站下载免费的PDF全文](/ch/ext_images/free.gif)
In order to investigate the regulatory effect of acupuncture on obesity patients with the Stomach-Intestine Excessive Heat Type, the pre-acupunctureal and post-acupunctural obesity index and biochemical indices of 718 simple obesity patients with Stomach-Intestine Excessive Heat Type were observed. It was showed that the marked weight loss effect was achieved in the cases by acupuncture, and the biochemical indices improved. It suggests that acupuncture had a optimal regulatory effect on the function of nerve, endocrine, digestion and energy metabolism. 相似文献
23.
Liu Wenen Tang Yin Wang Lange Tan Deming Xiangya Hospital Central South University Changsha 《中国现代医学杂志》2003,13(8):18-20
Extended -spectrum β -lactamases(ESBLs)aremainlyproducedbymembersofthefamilyEnter obacteriaceaewhichcanhydrolyzeβ -lactamantibi oticsincludingthethird - generationcephalosporinandaztreonam ,theESBLs- producingbacillishowedmedian -highresistancetoceftazidimeandaztreonamparticularly[1] .NowadaystheprevalenceofESBLs -producingstrainshavebeenreportedinmanyareasaroundtheworld[2 ] .ButthereisfewinformationabouttherelationshipbetweentheuseofantibioticandtheproductionofESBLs .Weperformedastud… 相似文献
24.
Effect of shenmai Injection on SIL-2R NK and LAK cells of patients with advanced carcinoma
下载免费PDF全文
![点击此处可从《中国结合医学杂志》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Serum interleukin-2 receptor (sIL-2R) level, activities of natural killer cell (NK) and lymphokine activated killer (LAK) cells were determined in 60 patients with advanced carcinoma (AC) before and after treatment with Shenmai Injection (SMI), forty healthy persons were taken as non-carcinoma control (NC). The results showed that: Serum sIL-2R level in AC were much higher than those in NC (P<0.05) and activities of NK and LAK cells in AC were much lower than those in NC (P<0.05) before treatment. There was no significant difference among gastric, colonic and lung cancer (P>0.05). After treatment with SMI, it was found that the level of sIL-2R in all patients were obviously lowered (P<0.05) while the activities of NK and LAK cells were signifficantly higher than that prior the treatment (P<0.05). Relevancy was not found between sIL-2R and NK, LAK cells. These data suggested that the immune function was compromised in AC, and SMI has a wide effect of immuno-regulation. 相似文献
25.
A study on various symptoms of chronic hepatitis treated with radix astragali and its regulative effect to levels of serum hormone
下载免费PDF全文
![点击此处可从《中国结合医学杂志》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Ke-zhou Liu Zhong-hang Tong Ming-tai Zhang Wei-zheng Gu Zhi-rong Huang Bi-he Shen Pei-ren Cai Gui-lang Zhu Wen-nan He Lan-sheng Feng Lie-ming Ding Zhi Chen Jian-hua Jin Xiu-zhi Zhang Qian-yan Yu Shui-jin Tong Ping Ye 《中国结合医学杂志》1995,1(2):118-121
Sixty-seven cases of chronic hepatitis were treated with Radix Astragali. After treatment (2-month course), the clinical improvement rate in 38 cases of the Stagnation of the Liver-Qi and Deficiency of the Spleen type was 92.1%, and in 26 cases of the Deficiency of Liver Yin and Kidney Yin type was 88.5%, more effective than in the control group (P<0.05). The regulative effect to the levels of serum hormone was observed in the patients of the Stagnation of the Liver-Qi and Deficiency of the Spleen type treated with this medicine. The results showed that the levels of serum triiodothyronine, estradiol (female) and testosterone (male) were increased after treatment (1.40±1.38 ng/dl, 129.30±1.23 pg/ml and 496.24±1.47 ng/dl). Pre-treatment levels were 1.22±1.49 ng/dl, 104.60±1.45 pg/ml and 398. 17±1.55 ng/dl respectively (P<0.05); however, the level of serum prolactin (2.75±4.46 ng/ml) was lower after treatment than before treatment (3.20±3.82 ng/ml,P<0.05). No obvious changes were observed in the levels of serum follicle stimulating hormone, luteinizing hormone, thyroid stimulating hormone, thyroxine, triiodothyronine uptake ratio and cortisol after treatment. 相似文献
26.
Zhao Xiao-zhong 《中国结合医学杂志》1995,(1)
TheEffectsofAstragalusmembranaceusandTripterygiumhypoglaucumonNKActivityinSystemicLupusErythematosusZhaoXiao-zhong(赵小忠)(Depar... 相似文献
27.
BACKGROUND: At present, researches on differentiating from human adipose-derived adult stromal cells (hADASC) to neuron-like cells are focus on inducing by artificial-synthetic compound solution; however, hippocampal astrocyte conditioned medium (HCAM) can induce in vitro differentiation from hADASC to neuron-like cells is still unclear.
OBJECTIVE: To observe whether HCAM can induce in vitro differentiation from hADASC to neuron-like cells.
DESIGN: Randomized control study.
SETTING: Department of Neurology, Taixing People's Hospital; Central Laboratory, North China Coal Medical College.
MATERIALS: Donor of adipose tissue was donated by female volunteers suffering from caesarean section in the department of obstetrics & gynecology in our hospital and aged 20-35 years. Adipose tissue was collected from subcutaneous tissue of abdomen during the operation. In addition, 8 male newborn Wistar rats within 24 hours with average body mass of 20 g were provided by Animal Institute of Chinese Academy of Medical Sciences. Rabbit-anti-human Nestin polyclonal antibody, rabbit-anti-human glial fibriliary acidic protein (GFAP) polyclonal antibody, rabbit-anti-human neuro-specific enolase polyclonal antibody and mouse-anti-human microtubal associated protein 2 (MAP-2) polyclonal antibody were provided by Wuhan Boster Company.
METHODS: The experiment was carried out in the Central Laboratory of North China Coal Medical College from October 2004 to June 2005. hADASC was cultured with HCAM and its growth and morphological changes were observed under inverted phase contrast microscope. Immunocytochemistry, immunofluorescence and Western blotting were used to evaluate the expressions of Nestin, which was a specific sign of nerve precursor, neuro-specific enolase and MAP-2, which was a specific sign of nerve cell, and GFAP, which was a specific sign of neuroglial cells.
MAIN OUTCOME MEASURES: Nestin, which was a specific sign of nerve precursor, neuro-specific enolase and MAP-2, which was a specific sign of nerve cell, and GFAP, which was a specific sign of neuroglial cells.
RESULTS: On the 3rd day of culture, partial hADASC started deformation from slender shuttle-shape cells to neuron-like cells. It suggested that cells stretched out apophysis, which were mainly double-pole or multiple-pole cells. Five days later, immunohistochemical detection suggested that expression of Nestin (10.5±0.037) was found out in cells; meanwhile, expressions of GFAP (38.4±0.052) and neuro-specific enolase (NSE) (15.7±0.023) were also found out in cells; however, expression of MAP-2 was not observed. Western blot indicated that, 5 days after effect of HCAM, Nestin was found out in hADASC; meanwhile, expressions of GFAP and neuro-specific enolase were also found out; however, expression of MAP-2 was not observed.
CONCLUSION: HCAM can induce the differentiation from hADASC to neuron-like cells in vitro. 相似文献
28.
BACKGROUND: Under the normal circumstance, there exist some synapses with inactive functions in central nervous system (CNS), but these functions are activated following nerve injury. At the early stage of brain injury, the abnormal functions of brain are varied, and they have very strong plasticity and are corrected easily.
OBJECTIVE: To observe the changes of neuronal morphology in hippocampal CA1 region and memory function in newborn rats with hypoxic-ischemic encephalopathy(HIE) from ischemia 6 hours to adult.
DESIGN: Completely randomized grouping, controlled experiment.
SETTING: Taian Health Center for Women and Children; Taishan Medical College.
MATERIALS: Altogether 120 seven-day-old Wistar rats, of clean grade, were provided by the Experimental Animal Center, Shandong University of Traditional Chinese Medicine. Synaptophysin (SYN) polyclonal antibody was provided by Maixin Biological Company, Fuzhou.
METHODS: This experiment was carried out in the Laboratory of Morphology, Taishan Medical College between October 2000 and December 2003. ① The newborn rats were randomly divided into 2 groups: model group and control group, 60 rats in each group. Five rats were chosen from each group at postoperative 6 hours, 24 hours, 72 hours, 7 days, 2 weeks and 3 weeks separately for immunohistochemical staining. Fifteen newborn rats were chosen from each group at postoperative 4 weeks and 2 months separately for testing memory ability (After test, 5 rats from each group were sacrificed and used for immunohistochemical staining)② The right common carotid artery of newborn rats of model group was ligated under the anesthetized status. After two hours of incubation, the rats were placed for 2 hours in a container filled with nitrogen oxygen atmosphere containing 0.08 volume fraction of oxygen, thus, HIE models were created; As for the newborn rats in the control group, only blood vessels were isolated, and they were not ligated and hypoxia-treated. ③ Thalamencephal tissue sections of newborn rats of two groups were performed DAB developing and haematoxylin slight staining. Cells with normal nucleous in 250 μm-long granular layer which started from hippocampal CA1 region were counted with image analysis system under high-fold optical microscope (×600), and the thickness of granular layer was measured. The absorbance (A) of positive reactant of SYN in immunohistochemically-stained CA1 region was measured. Learning and memory ability were measured with step through test 3 times successively. ④ t test and paired t test were used for comparing intergroup and intragroup difference of measurement data respectively, and Chi-square for comparing the difference of enumeration data.
MAIN OUTCOME MEASURES: Comparison of cytological changes in hippocampal CA1 region and memory ability at different postoperative time points between two groups.
RESULTS: Totally 120 newborn rats were involved in the result analysis. ① Cell morphological changes in hippocampal CA1 region: In the control group, with aging, perikaryon, nucleus and nucleolus in cortex of parietal lobe were significantly increased, Nissl body was compacted, the amount of neurons was declined, but the A of SYN positive reactant was relatively increased. In the model group, at postoperative each time point, neurons were seriously shrunk and dark-stained, nucleus was contracted, chromatin was condensed, nucleolus was unclear, even cells disappeared, especially the cells in 6 hours and 24 hours groups. The amount of neurons with normal morphology in hippocampal CA1 region and granular layer thickness in the model group at postoperative each time point were significantly less or smaller than those in the control group at postoperative 6 hours respectively (t =3.002-1.254, P < 0.01). The A value of SYN positive reactant at postoperative 2, 3 and 4 weeks was significantly higher than that at previous time point (t =2.011-2.716,P < 0.05-0.01). ② Test results of learning and memory ability: In the first test, there was no significant difference in the ratio of rats which kept memory ability between two groups (P > 0.05); In the third test, the ratio of rats which kept memory ability in the model group was significantly lower than that in the control group at postoperative 4 weeks and 2 months[53%(8/15),100%(15/15);60%(9/15),93%(14/15),χ 2=2.863,2.901,P < 0.01].
CONCLUSION: The destroyed hippocampal structure induces the decrease of learning and memory ability of developmental rats. Early interference can increase the quality of neurons and also promote functional development of the nervous system. 相似文献
29.
Xu Jingying Liu Xinling Ma Zhuhong Chen LingDepartment of Acupuncture & Moxibustion General Hospital of PLA Beijing China 《世界针灸杂志》1994,(1)
In this paper,250 cases of headache,shoulder pain,lumbago and leg pain weretreated with 20%Angelicae Sinensis mixed with Vitamine B_(12) point-injection therapy.The total effective rate was 95.2%and in which the cure rate was 53.6%.Authors have found a close correlation between the effectiveness and the duration of disease. 相似文献
30.
Ni Yan-xia 《中国结合医学杂志》1995,(1)
TherapeuticEffectofBerberineon60PatientswithNon-InsulinDependentDiabetesMellitusandExperimentalResearchNiYan-xia(倪艳霞);LiuAn-q... 相似文献