Clinical significance and prognosis of MYC translocation in diffuse large B-cell lymphoma

Recent studies have suggested that chromosomal aberrations of the MYC gene locus indicate an unfavorable prognosis in diffuse large B‐cell lymphoma (DLBCL). However, there have been few reports on MYC translocation in Chinese patients. One hundred and six cases of DLBCLs were analyzed using interphase fluorescent in situ hybridization. Immunophenotyping analysis (CD20, CD3, CD10, Bcl‐6, Mum‐1) was also performed. MYC translocation was identified in 13 (12.3%) out of 106 cases. All MYC⁺ DLBCLs showed a non‐germinal center B‐cell type. MYC⁺ DLBCLs showed significantly poorer overall survival (OS) and progression‐free survival, with a median OS and progression‐free survival time of 4.7 and 3.2 months, respectively (p < 0.001). Multivariate analysis using a Cox proportional hazard model confirmed that MYC⁺ (for OS, Hazards ratio 5.254; 95% CI, 2.354–11.723, p < 0.001) was the strongest independent predictor. DLBCL with MYC translocation is a subgroup of non‐germinal center B‐cell DLBCL with poor outcome. This may be a clinical characteristic that is specific to Chinese patients. Because only a few patients received rituximab, its usefulness could not be assessed. Future studies with larger numbers of patients are required. Copyright © 2011 John Wiley & Sons, Ltd.     

超脉冲等离子切除治疗高危重度前列腺增生

目的：探讨超脉冲等离子（PKSP）切除治疗高危重度前列腺增生（BPH）的疗效及安全性。方法：应用超脉冲等离子切除治疗高危重度BPH患者48例。结果：本组均安全耐受手术。手术时间40～120 min,平均67 min。灌注液量9 000～45 000 ml,平均9 850 ml。手术前后血清钠、Hb、HCT应用统计学处理,差异无统计学意义（P〉0.05）。术后3～5 d拔除导尿管,患者均顺利排尿。术后1个月时,IPSS评分下降至6～15分,平均6.8分;QOL平均降至2.0分;MFR上升到14～26 ml/s,平均上升17.6 ml/s,手术前后比较,差异均有统计学意义（均P〈0.01）。结论：PKSP切除治疗高危重度BPH的疗效确切,安全可靠。     

International Union of Basic and Clinical Pharmacology. LXXXV: calcium-activated chloride channels

Calcium-activated chloride channels (CaCCs) are widely expressed in various tissues and implicated in physiological processes such as sensory transduction, epithelial secretion, and smooth muscle contraction. Transmembrane proteins with unknown function 16 (TMEM16A) has recently been identified as a major component of CaCCs. Detailed molecular analysis of TMEM16A will be needed to understand its structure-function relationships. The role this channel plays in physiological systems remains to be established and is currently a subject of intense investigation.     

Biocompatible Poly(D,L‐lactide)‐block‐Poly(2‐methacryloyloxyethylphosphorylcholine) Micelles for Drug Delivery

Well‐defined amphiphilic PLA‐b‐PMPC diblock copolymers were synthesized. Bimimetic micelles were prepared and applied for release of anti‐cancer drugs (DOX). TEM and DLS analysis revealed a regular spherical shape with small diameter (less than 50 nm) of the micelle. The biocompatibility of PLA‐b‐PMPC micelles was studied, and it was found that the micelles possessed excellent cytocompatibility due to the zwitterionic phosphorylcholine group. DOX could be efficiently loaded into the micelles with a loading efficiency of 44–67%. The DOX‐loaded micelles showed lower cytotoxicity than free drugs and efficiently delivered and released the drug into cancer cells. With these properties, the PLA‐b‐PMPC polymer micelles are attractive as drug carriers for pharmaceutical application.

     

N-乙酰半胱氨酸保护PC12细胞对抗化学性低氧诱导的损伤

目的 探讨活性氧( ROS)清除剂N-乙酰半胱氨酸(NAC)能否保护PC12细胞对抗化学性缺氧引起的损伤.方法 应用化学性低氧模拟物氯化钴(CoCl2)处理PC12细胞,建立化学性缺氧损伤PC12细胞的实验模型.在CoCl2处理PC12细胞前60 min将NAC加入培养基中,作为预处理.应用CCK-8比色法检测细胞存活...     

广东省2005-2010年登革热流行特征分析

目的:掌握广东省登革热流行特征,为防控措施的制订提供科学依据。方法:收集国家疾病监测信息报告管理系统2005-2010年登革热基本信息,以及登革热病例个案流行病学调查资料与实验室检测结果,用描述性流行病学方法分析登革热发病的时间、空间、人群分布及血清学、病原学特征。结果:2005-2010年全省共报告登革热病例1718例,包括1568例本地感染病例和150例境外输入病例。共报告25起暴发事件,累计1311例病例,暴露总人数190余万。20～39岁成人发病率最高[(4.75～5.67)/100万],20岁以下低年龄组发病率较低。输入病例全年各月均有发生,94.7%的病例分布于广州、深圳等珠三角地区;本地感染病例则主要集中在8～10月,随年份不同分布于珠三角、粤东和粤西部分地区。2005-2010年从138份急性期血标本分离到67株登革热病毒,其中登革1型63株、登革2型2株、登革3型1株、登革4型病毒1株。期间采集监测点流行前、后期正常人血清标本2327份,检出登革热特异性抗体IgG阳性51份,总抗体阳性率2.19%。结论:广东省2005-2010年登革热输入病例与本地病例均有发生,发病患者群的流行病学与健康人血清抗体水平均没有体现登革热呈地方性的特征。     

深低温冷冻骨的形态学及免疫组织化学检测

背景:深低温冷冻技术能够降低同种异体骨的免疫原性,对修复骨缺损具有重要意义。目的:观察深低温冷冻对骨材料中骨形态发生蛋白2表达的影响。方法:18只新西兰兔随机分为3组,深低温冷冻3个月组,深低温冷冻6个月组,新鲜骨对照组,取右侧胫骨中上1/3,分别于-80℃保存保存3个月,6个月及不保存,进行苏木精-伊红染色和骨形态发生蛋白2免疫组织化学染色。结果与结论:深低温冷冻后骨材料形态学,与新鲜骨差异不大;深低温冷冻3个月及6个月骨组织中骨形态发生蛋白2均有表达,但较新鲜骨降低。