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目的 探讨银离子(Ag+)、铜离子(Cu2+)、锌离子(Zn2+)对5种常见念珠菌(白色念珠菌、热带念珠菌、近平滑念珠菌、克柔念珠菌和光滑念珠菌)的抗菌作用。方法 使用去离子水分别将硝酸银、氯化铜和氯化锌配制为0.1 mol·L-1的水溶液。取保存的白色念珠菌标准菌株ATCC-60193、近平滑念珠菌、光滑念珠菌、热带念珠菌、克柔念珠菌,加入溶菌肉汤培养液,使用比浊仪调配菌液浊度为0.5麦氏单位,然后使用无菌去离子水将菌液稀释至106 CFU·L-1。取配制好的0.1 mol·L-1的硝酸银、氯化铜和氯化锌溶液,分别倍比稀释为6个浓度(10-2、10-3、10-4、10-5、10-6、10-7 mol·L-1),分别取各浓度的金属离子溶液100μL与预制好的5种念珠菌悬液100μL进... 相似文献
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Ruiter DJ Roald B Underwood J Prat J;UEMS Section of Pathology/European Board of Pathology 《Virchows Archiv : an international journal of pathology》2004,444(3):278-282
Critical shortages of trained histopathologists limit the capacity of cancer and other clinical services. A survey of histopathology training in 18 European countries conducted by the European Union of Medical Specialists Section of Pathology/European Board of Pathology revealed a considerable shortage of both trained and trainee histopathologists in several of the responding countries. Demographic data indicate a high preponderance of trained histopathologists over 55 years of age and a notable proportion of part-time personnel. Although the training capacity in some countries has been increased, the immediate future of histopathology and the clinical services dependent on the specialty is worrisome. The histopathology workload is growing, and, in some countries, recruitment of trainees is insufficient for future needs. To avert the serious consequences for dependent clinical specialties and their patients, there should be a concerted European action to promote and expand histopathology training. 相似文献
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组织芯片是从组织块中挑选典型部位的组织以获得数百个圆柱状的样本,并按次序排列在一个新的石蜡块上,制作成HE切片,然后按研究的需要进行免疫组化及原位杂交等研究。组织芯片技术最重要的特点是:一次可以同时对多个组织块检测研究而对原来的组织材料破坏很小。此外,利用组织芯片仪还可以进行组织切片的精确定位和多种类型的分子生物学分析。 相似文献
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Zola H Swart B Boumsell L Mason DY;IUIS/WHO Subcommittee 《Journal of immunological methods》2003,275(1-2):1-8
The 7th International Workshop on Human Leucocyte Differentiation Antigens (HLDA7) studied a number of newly characterised molecules relevant to human leucocyte differentiation and function. The HLDA organisation, which devised and continues to maintain the CD nomenclature, is responsible, under the auspices of IUIS and WHO, for the nomenclature of all leucocyte differentiation markers. The 7th Workshop redefined a number of (principally carbohydrate) molecules, and assigned CD names to approximately 80 new molecules. This update lists, in tabular form, the redefined and newly assigned names, together with antibodies, which have been confirmed under Workshop conditions as specific for the new and redefined molecules. The major features of the cellular expression patterns are summarised, and a LocusLink accession number provided to enable the reader to access more detailed information through http://www.ncbi.nlm.nih.gov/LocusLink. 相似文献
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Ekouevi DK Rouet F Becquet R Inwoley A Viho I Tonwe-Gold B Bequet L Dabis F Leroy V;ANRS / Ditrame Plus Study Group 《Journal of acquired immune deficiency syndromes (1999)》2004,36(2):755-757
The aim of this study performed in Abidjan, C?te d'Ivoire, was to describe the distribution of CD4+ T-cell lymphocytes (CD4) in HIV-1-infected (HIV+) pregnant women diagnosed during prenatal voluntary counseling and testing and to assess whether HIV-related immunodeficiency influenced the acceptance of an antiretroviral (ARV) package (zidovudine beginning at 36 weeks of amenorrhea plus intrapartum nevirapine) to prevent mother-to-child transmission. Between April and June 2002, a CD4 count was systematically performed in all HIV+ women (n=221) in 5 antenatal clinics carrying out voluntary counseling and testing. No difference in CD4 count was found in HIV+ women who did not return for their test result (n=50) and those who were informed of their positive serostatus (n=171) (median CD4 count: 389/mm3 vs. 420/mm3; P=0.19). We also found a lack of difference in CD4 count in those who accepted ARV (n=72) and those who did not but knew their HIV status (n=99) (median CD4 count: 405/mm3 vs. 425/mm3; P=0.47). The overall uptake of the intervention (31.9%) appeared to be independent of the maternal immune status. 相似文献
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Yamanaka H Teruya K Tanaka M Kikuchi Y Takahashi T Kimura S Oka S;HIV/Influenza Vaccine Study Team 《Journal of acquired immune deficiency syndromes (1999)》2005,39(2):167-173
Influenza vaccine is recommended for HIV-1-infected patients. The present prospective study was conducted to evaluate the clinical efficacy and immunologic responses to the vaccine. From November 1 to December 27, 2002, 262 HIV-1-infected patients received a trivalent influenza subunit vaccine, whereas 66 did not. Influenza illness occurred in 16 vaccinated and 14 nonvaccinated patients (incidence = 6.1% [95% confidence interval (CI): 4%-10%] in vaccinated vs. 21.2% [CI: 13%-35%] in nonvaccinated persons, P < 0.001; relative risk = 0.29 [CI: 0.14-0.55]). Influenza vaccine provided clinically effective protection against influenza illness in HIV-1-infected patients. In baseline antibody-negative patients, anti-H1 and anti-H3 antibody responses to the vaccination were significant in those patients with a CD4 count >200 cells/muL compared with those with a CD4 count <200 cells/muL (P < 0.05). In contrast, in baseline antibody-positive patients, good antibody responses were observed irrespective of CD4 counts, like the healthy controls. Based on these results, annual vaccination is recommended. Specific CD4 responses correlated with HIV-1 viral load (VL), especially in patients treated with highly active antiretroviral therapy (HAART) compared with those without HAART (P < 0.01), although the clinical efficacy did not correlate with HIV-1 VL. HAART may enhance the immunologic efficacy of influenza vaccine. 相似文献