以右心衰为首发症状的右室流出道平滑肌肉瘤1例

 平滑肌肉瘤常常发生于富含平滑肌的气管,发生于心脏的平滑肌肉瘤极为少见,本例发生在右室流出道,并累及肺动脉瓣以右心衰为首发症状临床罕见。     

玻璃酸钠治疗肩周炎的临床应用

目的：探讨佰备玻璃酸钠（上海景峰制药有限公司）治疗肩周炎的临床应用。方法：采用玻璃酸钠关节腔内注射并进行临床观察。结果本组320例病人治愈260例（81．2％）,好转50例（15．6％）,无效10例（3．2%）均为冻结期的病例,有效率为96．8％。结论：玻璃酸钠关节腔内注射治疗肩周炎疗效较好,值得临床推广。     

淫羊藿苷对线粒体损伤模型大鼠脑内β-淀粉样蛋白和神经营养因子的影响

该研究主要是观察中药淫羊藿苷(ICA)对线粒体损伤模型大鼠脑内β-淀粉样蛋白(Aβ)含量和神经营养因子表达的影响.SD大鼠随机分为假手术组、模型组、ICA低、高剂量组(12,36 mg· kg-1).各组大鼠皮下埋植Alzet微泵,假手术组给予生理盐水,其余各组给予线粒体呼吸链复合体Ⅳ抑制剂叠氮钠(0.Smg·kg-1 ·h-1),共28 d,建立大鼠线粒体损伤模型.生化法测定大鼠海马线粒体复合体Ⅳ(即细胞色素C氧化酶)活性;EHSA法测定大鼠脑内Aβ含量;Westem blot法和免疫组织化学法测定大鼠脑内神经营养因子及其受体的表达.结果显示叠氮钠微泵灌注可引起模型大鼠脑内线粒体细胞色素C氧化酶(COX)活性明显下降,Aβ含量升高,神经生长因子(NGF)、脑源性神经营养因子(BDNF)及其受体TrkB的表达减少.ICA灌胃给药明显改善模型大鼠的上述异常.结果表踢ICA能够增强叠氮钠模型大鼠脑内线粒体活性,抑制Aβ产生,增强神经营养因子表达,提示ICA具有良好的治疗阿尔茨海默病的应用前景.     

椎体加压钉固定对脊柱侧凸山羊椎体生长板影响的组织学观察

目的:定量分析椎体加压钉(Staple)固定矫正山羊脊柱侧凸后椎体生长板的组织形态学变化,探讨Staple同定对侧凸椎体生长板的影响.方法:用单侧椎弓根螺钉不对称拴系的方法对10只7～10周龄雌性山羊建立脊柱侧凸模型.术前、术后均行脊柱后前位和侧位X线平片检查,术后每4周检查一次,观察山羊脊柱弯曲情况.8～10周后,将成功建立脊柱侧凸模型的山羊随机分为对照组和Staple治疗组,两组山羊均取出后路拴系,Staple治疗组取出拴系后在最大弯曲椎体的凸侧前路置入记忆合金Staple.继续观察8周后完整获取两组山羊的顶椎上生长板、顶椎上一个椎体的下生长板以及两者之间的椎间盘,聚甲基丙烯酸甲酯(PMMA)包埋,不脱钙切片,甲苯胺蓝染色,镜下测量生长板生长方向上增殖层、肥大层高度.结果:10只山羊中有9只术后8～10周内进展为明显的脊柱侧凸,Staple治疗组(n=5)和对照组(n=4)的Cobb角分别为47.2°±11.2°、51.0°±17.7°,两组无显著性差异(P>0.05).Staple治疗组在Staple置入后即刻Cobb角为34.8°±12.4°,与置入前比较无明显差异(P>0.05);置入后8周为15.6°±11.7°,与置入后即刻比较明显变小(P<0.05);对照组在拴系取出术后即刻和术后8周Cobb角无明显变化(P>0.05).Staple治疗组生长板凸侧肥大层高度为29.17±4.38μm.凹侧为42.46±8.43μm,两侧有显著性差异(P<0.05);凸侧增殖层高度为75.70±18.36μm,凹侧为81.65±12.49μm,两侧无显著性差异(P>0.05).对照组生长板肥大层与增殖层高度两侧均无显著性差异(P>0.05).结论:Staple固定矫正脊柱侧凸重要的细胞学机制可能是逆转了生长板肥大层两侧原有的差异,使凸侧高度低于凹侧.     

新诊断2型糖尿病患者体重与血脂的相关性研究

目的探讨新诊断2型糖尿病(T2DM)患者体重与血脂谱的特点。方法对136例新诊断T2DM患者作为研究对象,根据体重指数(BMI)分为体重正常组、超重组和肥胖组;又根据腰围及腰臀比分为正常组及肥胖组,分别比较各组间血脂谱的特点。结果新诊断的(T2DM)患者中血脂异常117例(86.03%),与正常组比较,肥胖组中甘油三脂(TG)、总胆固醇(TC)、低密度脂蛋白(LDL)及尿酸(UA)较正常组增高,其中TG、TC及UA差异有统计学意义(P<0.05)。结论新诊断T2DM患者血脂异常发生率高,且随体重增加,与TG、TC及UA升高呈正相关。     

Expression of intercellular cell adhesion molecule 1 and matrix metalloproteinase 9 in endometrium of pregnant mice with long-term exposure to low concentration of sevoflurane北大核心

BACKGROUND: Epidemiological studies have found that female workers who are exposed to waste sevoflurane for a long time have reduced fertility and increased incidence of abortion and fetal deformity. OBJECTIVE: To imitate the working environment of long-term exposure to waste sevoflurane and investigate the mechanism of embryo implantation disorder induced by low-concentration sevoflurane exposure by observing the expression of intercellular adhesion molecule 1, integrin β1, P-selectin, and matrix metalloproteinase 9 in endometrium of pregnant mice exposed to low concentrations of sevoflurane, attempting to provide a basis for clinical safe drug use and occupational protection and to lay a foundation for further research on the mechanism of inhalation anesthetics on embryo implantation at gene and molecular levels. METHODS: Forty female Kunming mice and sixteen male Kunming mice, aged 6 weeks, weighting (20±2) g, were caged separately. Forty female mice were randomly divided into experimental group and control group. Mice in the experimental group were exposed to 0.1% sevoflurane, 6 hours per day, while those in the control group were exposed to the air. Thirty days later, the female mice in estrus were caged with mature male mice at a rate of 2:1. Whether the female mice were pregnant was observed at 7:00 am on the second day after mating. The pregnant mice were kept independently in the original condition (n ≥ 8 pregnant mice in each group). Mouse uterus on day 4.5 of gestation was removed and sliced for histological observation. The expression of intercellular adhesion molecule 1, integrin β1, P-selectin, and matrix metalloproteinase 9 in endometrial tissue was detected by immunohistochemistry and statistically analyzed. The average integrated absorbance value of positive reactants was calculated. RESULTS AND CONCLUSION: In the experimental and control groups, 17 and 15 female mice were respectively found in estrus; 12 and 10 female mice respectively were found vaginal plugs after mating. Five and two female mice were found pseudopregnant on day 4.5 of gestation in the experimental and control groups, respectively. Therefore, there were 7 and 8 pregnant mice in the experimental and control groups, respectively, and the vacant value in the experimental group was replaced by the average value obtained in the same group, which would be subsequently used in the controlled trial. Immunohistochemical results showed that the endometrium (glandular epithelial cells, luminal epithelial cells and stromal cells) of all pregnant mice were positively stained brownish yellow, but the expressions of intercellular adhesion molecule 1, integrin β1, P-selectin, and matrix metalloproteinase 9 (0.019±0.007, 0.017±0.007, 0.015±0.005, 0.012±0.005) in the experimental group were significantly lower than those in the control group (0.032±0.014, 0.025±0.008, 0.021±0.007, 0.023±0.005) (P < 0.05). All these findings indicate that long-term exposure to 0.1% sevoflurane may affect the adhesion of mouse embryos to endometrium and the embryo implantation, which may be related to the inhibitory effects of sevoflurane on the expressions of intercellular adhesion molecule 1, integrin β1, P-selectin, and matrix metalloproteinase 9, causing the imbalance in endometrial immunoregulation, the invasion of trophoblast cells into the endometrium and the inhibition of endometrial decidualization, and miscarriage and bleeding due to damaged vascular endothelium. © 2023, Publishing House of Chinese Journal of Tissue Engineering Research. All rights reserved.     

合欢皮中三萜皂甙元的研究

合欢皮中三萜皂甙元的研究陈四平张如意（北京医科大学植化教研室，北京１０００８３）中药合欢皮（Ａｌｂｉｚｚｉａｅｃｏｒｔｅｘ）为合欢（ＡｌｂｉｚｚｉａｅｊｕｌｉｂｒｉｓｉｎＤｕｒａｚｚ）树皮。具解郁安神，活血消肿功能。用于心神不安，忧郁失眠，肺痈疮肿...     

白芷化学成分的分离和鉴定

近年来8-甲氧基补骨脂素(8-methoxy-psoralen)配合黑光(或长波紫外线)照射治疗银屑病、白癜风的光化学疗法,被认为是治疗这类皮肤病的较好方法之一。为了寻找8-甲氧基补骨脂素或其代用品,我们进行了白芷中呋喃骈香豆素类成分的提取、分离和鉴定。     

2型糖尿病合并脑梗死相关危险因素

INTRODUCTION Incidence of type 2 diabetes combined with cerebral infarction in-crease with increase of age. High blood sugar, hypertension, hyper-cholesterolemia and high LDL are main risk factors to cerebral in-farction. Obesity is also the risk factor to diabetes combined withcerebral infarction.