Assessment of the “fish tumors or other deformities” beneficial use impairment in brown bullhead (Ameiurus nebulosus): II. Liver neoplasia

Liver pathology of fishes, including neoplastic and preneoplastic lesions, is widely used as an indicator of exposure to anthropogenic contaminants. By definition, the “fish tumor or other deformities” beneficial use impairment (BUI) at Great Lakes Areas of Concern (AOC) includes neoplastic and preneoplastic liver lesions in brown bullhead (Ameiurus nebulosus) or suckers. Unfortunately, adequate guidelines for defining neoplastic and preneoplastic liver lesions or determining rates at unimpacted control sites were not provided and different criteria have been used. In some cases, only neoplastic changes were used to calculate tumor prevalence, in some both neoplastic and preneoplastic changes and in some it is difficult to determine which changes were included. Using standardized criteria, the prevalence of liver neoplasia was compared at eight AOC during 1998–2000. The Cuyahoga River had the highest prevalence (25.0%), while the Maumee River had the lowest (3.9%). The Buffalo (4.8%), Detroit (5.9%), Ashtabula (6.8%), Niagara (7.5%) and Black (8.9%) rivers were intermediate, as was Presque Isle Bay (7.1%). From 2002 to 2007 the prevalence of liver neoplasia at Presque Isle Bay ranged from a low of 2.1% (2002) to a high of 12.0% (2007). Non-AOC sites, as potential reference sites, also were monitored during this time. By combining years and sites, the prevalence of liver neoplasia in bullhead (aged 2 to 12 years) at inland lakes was 0.7%, at bays/harbors was 1.6% and at tributary sites was 4.1%. This is the same trend (inland lakes < bays/harbors < tributaries < Presque Isle Bay) noted for orocutaneous neoplasms.     

Images for accelerating architectural walkthroughs

We describe our image-based solutions to produce a system for the interactive walkthrough of large architectural models. Our overall approach uses a specialization of impostors introduced by Maciel and Shirley (1995). With this method, portions of a general 3D model can be replaced with representations that take less time to render, namely 2D textures. Solving the general problem of deciding where to place textures to improve rendering performance proves difficult. The cells and portals framework lets us formulate a set of concrete and efficient algorithms for replacing geometry with images     

3H]naltrindole: a potent and selective ligand for labeling delta-opioid receptors

Naltrindole (NTI) is a selective and potent delta-opioid antagonist which preferentially antagonizes a subset of selective delta-opioid agonists. The purpose of this study was to evaluate whether [3H]NTI, the first radiolabeled delta-opioid antagonist, could selectively label delta-opioid receptors in a synaptosomal preparation. Increasing temperature and protein concentration (0.1-1.6 mg protein) increased the specific binding of [3H]NTI. Monovalent and divalent cations (0.01-100 mM) had minimal effects on the binding properties of [3H]NTI, in contrast to their effects on binding of the delta agonists [3H]DPDPE and [3H]DSLET. Subfractionation of rat brain homogenates revealed that [3H]NTI and [3H]DSLET primarily labeled binding sites in synaptosomal and microsomal fractions, whereas [3H]DPDPE labelled half as many sites in synaptosomal fraction. The Bmax determined for [3H]NTI in crude synaptosomal fraction was 95 +/- 12 fmol/mg. The dissociation constant (Kd) was determined from three different methods to be 0.08 +/- 0.02 nM (Scatchard analysis), 0.07 +/- 0.02 nM (competition study) and 0.03 +/- 0.005 nM (kinetic analysis). [3H]NTI binding was not significantly inhibited by mu- or kappa-opioid ligands or by nonopioid compounds. These results demonstrate that [3H]NTI is a potent and selective radioligand for delta-opioid receptors in rat brain preparations.     

A novel demodulator/detector for digital and analog signals on LMRchannels

The design, implementation, and performance of an all-digital demodulator/detector suitable for differential phase-shift keying (DPSK), continuous-phase frequency-shift keying (CPFSK), frequency-shift keying (FSK), and analog FM are discussed. In this modulator/detector, two detectors, one noncoherent and another differentially coherent, operate simultaneously to provide data detection and automatic frequency control (AFC). Test results indicate that the system provides improved performance over the conventional analog quadrature detector for two-period raised-cosine (2RC) CPFSK modulation in additive white Gaussian noise (AWGN) and Rayleigh fading channels. Being all-digital, the demodulator/detector is well suited for integrated circuit implementation. In addition, the system performs as well as the analog quadrature detector for analog FM voice transmissions, thereby maintaining full compatibility with analog land mobile radio (LMR) transmissions     

Amorphous phase separation of ionomer glasses

Ionomer glasses of generic composition SiO₂-Al₂O₃-P₂O₅-CaO-CaF₂ were studied using differential scanning calorimetry (DSC) and high temperature dynamic-mechanical thermal analysis (DMTA). High temperature DMTA was used to measure the glass transition temperatures (T _g) of the original starting glass compositions, as well as being able to follow amorphous phase separation (APS) within the glass. High temperature DMTA traces of all the glasses studied exhibited two maxima in tan . These maxima correspond to two glass transition temperatures and demonstrate that amorphous phase separation of the parent glass into two glass phases had occurred. A DMTA study of a Sodium-Boro-Silicate glass, which is known to undergo amorphous phase separation yielded similar results. DSC studies showed that the ionomer glasses underwent a nucleation process at temperatures just above the glass transition temperature which is probably associated with APS. The glasses exhibited optimum nucleation temperatures which moved to lower temperatures with longer hold times indicating the time dependency of the APS process.     

Tryptophan in bovine rhodopsin: its content, spectral properties and environment

The tryptophan content of purified bovine rhodopsin was obtained by two independent methods: direct analysis of hydrolysates prepared by digestion of opsin with methanesulfonic acid containing 0.2% 3-(2-aminoethyl)indole and a computer-assisted analysis of the near-UV spectrum of rhodopsin. Both methods gave a value of eight tryptophan residues per rhodopsin. Based on the near-UV spectral analysis, the light-induced difference spectrum of rhodopsin, and the susceptibility of residues to oxidation by N-bromosuccinimide, we concluded that approximately half of the tyrosine and tryptophan residues are shielded to some extent from the aqueous solvent, that two of the tryptophan residues are in very apolar environments, and that following light excitation at least one of these tryptophan residues and several tyrosines are exposed to an aqueous environment. Analysis of rhodopsin absorption in the far-UV indicated that below 240 nm, approximately half of the absorption is due to aromatic residues and that the other half is largely due to the peptide bond. The effect of illumination on secondary structure is to induce a loss in helical structure, calculated to involve 35% of the amino acid residues in purified rhodopsin. If light-induced changes in secondary structure are specifically excluded, most of these results can be extended to bovine rod outer segment membranes.     

On the origin of transverse incoherence in Z-contrast STEM

We use a Bloch wave approach to further investigate the origins of the incoherent nature of Z-contrast imaging using an ADF detector in a STEM. We discuss how, although at high angles the collected electrons will be mostly thermally scattered in addition to the elastic scattering, it is not the thermal scattering that destroys the coherence, rather the combination of the large detector with the high-angle elastic scattering. This incoherent nature of the elastic scattering arises through the filtering of the 1s-type Bloch states by the detector geometry. We show that it is this filtering that renders an atomic column an independent scatterer insensitive to the configuration of neighbouring columns. It also makes the image contrast insensitive to the effects of beam spreading onto neighbouring columns as the probe propagates through the crystal. We also discuss the implications of this for previous calculations of the intensity of Z-contrast images.     

Perceptions of organizational change: A stress and coping perspective.

Few organizational change studies identify the aspects of change that are salient to individuals and that influence well-being. The authors identified three distinct change characteristics: the frequency, impact and planning of change. R. S. Lazarus and S. Folkman's (1984) cognitive phenomenological model of stress and coping was used to propose ways that these change characteristics influence individuals' appraisal of the uncertainty associated with change, and, ultimately, job satisfaction and turnover intentions. Results of a repeated cross-sectional study that collected individuals' perceptions of change one month prior to employee attitudes in consecutive years indicated that while the three change perceptions were moderately to strongly intercorrelated, the change perceptions displayed differential relationships with outcomes. Discussion focuses on the importance of systematically considering individuals' subjective experience of change. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Use of transient enhanced diffusion to tailor boron out-diffusion

We report experimental results demonstrating the use of transient enhanced diffusion (TED) caused by silicon implant for “tuning” boron out-diffusion. The effect was measured as a function of the silicon implant dose and anneal temperature, and a range of boron junction depth movement from almost none up to 81 nm was observed with increasing TED at 750°C. The diffused profiles could be approximated by using a modified solubility limit model to describe the enhanced boron diffusion and clustering. However, by using a more sophisticated continuum model based on atomistic calculations, excellent agreement with the measured profiles could he obtained. In addition, the fit to the measured data yields the fraction of boron present in BI₂ precursor clusters after silicon implant as a function of the silicon implant dose. Two possible applications of the TED “tuning” are discussed, with device simulations which show that the effect is sufficiently large to tune the base width of a bipolar device from being depleted to that suitable for a high performance device     

Towards the disintermediation of creative music search: analysing queries to determine important facets

Creative professionals search for music to accompany moving images in films, advertising, television. Some larger music rights holders (record companies and music publishers) organise their catalogues to allow online searching. These digital libraries are organised by various subjective musical facets as well as by artist and title metadata. The purpose of this paper is to present an analysis of written queries relating to creative music search, contextualised and discussed within the findings of text analyses of a larger research project whose aim is to investigate meaning making in this search process. A facet analysis of a collection of written music queries is discussed in relation to the organisation of the music in a selection of bespoke search engines. Subjective facets, in particular Mood, are found to be highly important in query formation. Unusually, detailed Music Structural aspects are also key. These findings are discussed in relation to disintermediation of this process. It is suggested that there are barriers to this, both in terms of classification and also commercial/legal factors.