Book reviews

E.T. Layton with R Pinneau and J. Costello, And I Was There. Pearl Harbor and Midway (New York: William Morrow), pp. 596, $19.95.

Donald A. Torres, Handbook of Federal Police and Investigative Agencies (Westport, Conn., and London: Greenwood Press, 1985), pp. 411, £49.95.

Ernest R. May (ed.), Knowing One's Enemies. Intelligence Assessment Before the Two World Wars (Princeton: Princeton University Press, 1984), pp. 561, $29.50 or £19.80 (cloth); $14.50 or £9.70 (paper).     

Distinguishing minisatellite mutation from non-paternity by MVR-PCR

Minisatellite variant repeat (MVR) mapping using the polymerase chain reaction (PCR) was devised to map the interspersion pattern of subtle variant repeats along minisatellite tandem arrays. MVR-PCR has revealed enormous diversity of allele structures at several loci, far more than can be resolved by allele length analysis. We have reported the application of MVR-PCR at minisatellite MS32 (D1S8) and MS31A (D7S21) in a paternity case lacking a mother and showed that it resulted in higher paternity probabilities than for a set of 12 other DNA markers including six STRs. Hypervariable minisatellites like MS32 and MS3lA can however, show significant germline mutation rates to new length alleles which can generate false exclusions in paternity cases although paternity cases showing mutant paternal alleles at more than one locus will be rare when several MVR loci are examined. Detailed knowledge of mutation processes coupled with MVR analysis of allele structure can help distinguish mutation from non-paternity. We now show how similar mutant alleles are to their progenitors using both real and simulated data, and demonstrate how MVR-PCR can be used to identify mutant paternal allele in paternity cases showing apparent exclusions.     

The potential contribution of MVR-PCR to paternity probabilities in a case lacking a mother.

Minisatellite variant repeat (MVR) mapping using the polymerase chain reaction (PCR) was applied to a paternity case lacking a mother to evaluate the paternity probability. After three flanking polymorphic sites at each of MS31A and MS32 loci were investigated from the child and alleged father, allele-specific MVR-PCR was performed using genomic DNA. It was confirmed that one allele in the child was identical to that in the alleged father at both loci. Mapped allele codes were compared with allele structures established from population surveys. No perfect matches were found although some motifs were shared with other Japanese alleles. The paternity index and probability of paternity exclusion at these two MVR loci were then estimated, establishing the power of MVR-PCR even in paternity cases lacking a mother.     

Highly polymorphic minisatellite sequences: allele frequencies and mutation rates for five locus-specific probes in a Caucasian population

Six human minisatellite sequences (MS1, MS8, MS29, MS31, MS43, g3) have been subcloned into a stable host/vector system. Allele frequencies at the hypervariable loci detected by five of these probes were determined in a Caucasian population (200 individuals). Mendelian inheritance has been demonstrated in 5 large multi-generation pedigrees. The mutation rate has been determined in 59 families. The highest mutation rate was observed with MS1, as might be predicted from the observed high heterozygosity and in agreement with previous direct measurement of germ line mutation rates. The data presented on allele frequencies and mutation rates provide preliminary data supporting the use of these probes in paternity analysis and forensic investigations.     

‘Free from all uninvited touch of man’: Women's campaigns around sexuality, 1880–1914

The history of sex in the last 100 years has generally been represented as a triumphant march from Victorian prudery into the light of sexual freedom. From a feminist perspective the picture is different. During the last wave of feminism women, often represented as prudes and puritans by historians, waged a massive campaign to transform male sexual behaviour in the interests of women. They campaigned against the abuse of women in prostitution, the sexual abuse of children, and marital rape. This article describes the women's activities in the social purity movement, and the increasingly militant stance taken by some pre-war feminists who refused to relate sexually to men, in the context of the developing feminist analysis of sexuality. The main purpose of the paper is to show that in order to understand the significance of this aspect of the women's movement we must look at the area of sexuality not merely as a sphere of personal fulfilment but as an arena of struggle in which male dominance and women's subordination can be most powerfully reinforced and maintained or fundamentally challenged.