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61.
Zörntlein SW Kopp A Becker J Kaufmann TJ Röhrich J Urban R 《Forensic science international》2012,214(1-3):113-117
The in vitro production of GHB was observed in freshly collected, untreated whole blood samples using glass BD-Vacutainers and polypropylene S-monovettes. GHB concentrations were determined daily over a period of one week and after 3, 6 and 9 weeks again. Furthermore, the GHB concentration in 40 untreated random whole blood samples stored at 4°C for a longer period of time (10 samples 12 month, 10 samples 24 month and 20 samples 36 month) was also determined. For comparison, the in vitro production of GHB in freshly collected and prepared serum samples was observed. GHB serum concentrations were determined three times over a period of one week and once again after six weeks. Sample preparation was performed by means of methanolic extraction following the precipitation of whole blood and serum samples. A methanolic standard calibration was done in a low range of 0.005-0.1 μg/mL (LOD: 0.004, LLOQ: 0.013). For quantification a spiked blood bank serum with a determined GHB concentration of 0.09 μg/mL was used. Corrected calibrations in the range of 0.09-5.09 μg/mL were used (LOD: 0.08 μg/mL, LLOQ: 0.30 μg/mL), recovery: 91.3% (high level: 4.09 μg/mL) 50.5% (low level: 0.19 μg/mL). RESULTS: Relevant elevation of GHB was observed in all whole blood samples stored in liquid form (4°C or room temperature). In two of the 40 whole blood samples stored over a longer period of time at 4°C, GHB concentrations in the range of 13 μg/mL were even determined. These findings constitute grounds for caution. Even a GHB cut-off level of 5 μg/mL cannot be considered as "absolutely positive" proof of a case of exogenous administration, at least in untreated liquid blood samples in long time storage. However, no significant elevations of GHB were otherwise observed in any of the serum samples independently of storage temperature nor in the whole blood samples that were frozen for storage. CONCLUSIONS: The results suggest that the cut-off for exogenous GHB of 5 μg/mL could be lowered significantly, with the consequence of winning valuable time for the potential victim, but only if serum is collected for GHB determination or if the whole blood sample is frozen immediately after collection and the procedure well documented. 相似文献
62.
Massonnet G Buzzini P Monard F Jochem G Fido L Bell S Stauber M Coyle T Roux C Hemmings J Leijenhorst H Van Zanten Z Wiggins K Smith C Chabli S Sauneuf T Rosengarten A Meile C Ketterer S Blumer A 《Forensic science international》2012,222(1-3):200-207
A collaborative study on Raman spectroscopy and microspectrophotometry (MSP) was carried out by members of the ENFSI (European Network of Forensic Science Institutes) European Fibres Group (EFG) on different dyed cotton fabrics. The detection limits of the two methods were tested on two cotton sets with a dye concentration ranging from 0.5 to 0.005% (w/w). This survey shows that it is possible to detect the presence of dye in fibres with concentrations below that detectable by the traditional methods of light microscopy and microspectrophotometry (MSP). The MSP detection limit for the dyes used in this study was found to be a concentration of 0.5% (w/w). At this concentration, the fibres appear colourless with light microscopy. Raman spectroscopy clearly shows a higher potential to detect concentrations of dyes as low as 0.05% for the yellow dye RY145 and 0.005% for the blue dye RB221. This detection limit was found to depend both on the chemical composition of the dye itself and on the analytical conditions, particularly the laser wavelength. Furthermore, analysis of binary mixtures of dyes showed that while the minor dye was detected at 1.5% (w/w) (30% of the total dye concentration) using microspectrophotometry, it was detected at a level as low as 0.05% (w/w) (10% of the total dye concentration) using Raman spectroscopy. This work also highlights the importance of a flexible Raman instrument equipped with several lasers at different wavelengths for the analysis of dyed fibres. The operator and the set up of the analytical conditions are also of prime importance in order to obtain high quality spectra. Changing the laser wavelength is important to detect different dyes in a mixture. 相似文献
63.
Amy Z. Mundorff Ph.D. Eric J. Bartelink Ph.D. Turhon A. Murad Ph.D. 《Journal of forensic sciences》2014,59(4):891-897
Previous research has demonstrated significant sexual dimorphism in friction ridge skin characteristics. This study uses a novel method for measuring sexual dimorphism in finger ridge breadths to evaluate its utility as a sex estimation method from an unknown fingerprint. Beginning and ending in a valley, the width of ten parallel ridges with no obstructions or minutia was measured in a sample of 250 males and females (N = 500). The results demonstrate statistically significant differences in ridge breadth between males and females (p < 0.001), with classification accuracy for each digit varying from 83.2% to 89.3%. Classification accuracy for the pooled finger samples was 83.9% for the right hand and 86.2% for the left hand, which is applicable for cases where the digit number cannot be determined. Weight, stature, and to a lesser degree body mass index also significantly correlate with ridge breadth and account for the degree of overlap between males and females. 相似文献
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运用微量热启动PCR技术,对20例正常家系的遗传学分析,证实D17530位点的遗传符合孟德尔遗传规律,表现为简单的共显性遗传。同时,对100例亲子鉴定案例进行回顾性分析研究,证明D17S30位.在多态性可应用于我国法医学亲子鉴定。根据D17S30位点基因频率估算的排除概率(74.04%)与其实际排除能力(80.00%)无显著差异。在15倒排除亲子关系的案例中,有2例由D17S30位点单独取得排除证据。D17S30位点是法医学上有重要意义的遗传学标记,可用于亲子鉴定。 相似文献
67.
High achievers are a unique subset of persons referred for neuropsychological and psychological assessments in medicolegal contexts, including personal injury, disability, and workers’ compensation cases. Literature in this area is limited and poorly integrated, and neither a conceptual model nor best evidence-informed practices for the assessment of high achievers in the medicolegal context have yet emerged. Construct and methodological issues also hamper the ability of assessors to accurately and fairly assess this specialist group, which is particularly troubling in high-stakes forensic assessments. This paper reviews the current methodological issues involved in the assessment of high achievers and proposes an integrated, multimethod assessment model based on a particular vocational group, namely that of business leaders and entrepreneurs, that draws from available empirical research in different fields of psychological inquiry. 相似文献
68.
Morality policies-such as those pertaining to abortion, capitalpunishment, gambling, gay rights, pornography, and sex educationgenerateconflict over core values. These policies tend to be technicallysimple, potentially salient to the general public, and involvehigher than normal levels of citizen participation. They are,therefore, highly susceptible to being thrust into the activephase of policymaking when an incongruity develops between publicopinion and public policy. The reservation of authority to thestates on these issues embodied in the Tenth Amendment to theU.S. Constitution allows for closer policy-opinion congruence,therefore stabilizing morality policy politics. However, recentfederal actions on a range of morality policies have thrustthese issues onto the active political agenda of the states. 相似文献
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70.
This study of the impact of fair trade relies on new field data from coffee and banana co-operatives in Peru and Costa Rica, including a detailed assessment of its welfare effects by comparing FT farmers with non-FT farmers as a benchmark. Attention is focused on three major effects: (a) direct tangible impact of FT arrangements on the income, welfare, and livelihoods of rural households; (b) indirect effects of fair trade for improving credit access, capital stocks, investments, and attitudes to risk; and (c) institutional implications of fair trade for farmers' organisations and externalities for local and regional employment, bargaining, and trading conditions. Although direct effects in terms of net income remain fairly modest, important benefits are found to include capitalising farmers and strengthening their organisations. 相似文献