Antigens of Mycobacterium tuberculosis Recognized by Antibodies during Incipient, Subclinical Tuberculosis

Serum samples obtained from human immunodeficiency virus (HIV)-infected tuberculosis (TB) patients months prior to clinical TB were used to delineate the profile of Mycobacterium tuberculosis culture filtrate proteins recognized during subclinical TB. A subset of ~12 antigens was recognized by antibodies in these serum samples. Antibodies to two of these antigens (81 [88]-kDa malate synthase [GlcB] and MPT51) were present in serum samples obtained during incipient subclinical TB in 19 (~90%) of the 21 HIV-infected TB patients tested. These antigens will be useful for devising diagnostic tests that can identify HIV-positive individuals who are at a high risk for developing clinical TB.     

新型头孢菌素类抗生素

近10年来，抗生素耐药性问题日趋严重。头孢菌素类抗生素因具广谱抗菌活性和低毒性而常被用于治疗细菌感染，而且能安全地用于那些对青霉素过敏而依赖于此类抗生素的病人，但由于其耐药菌株的不断增加，这类药物用于一线常规治疗已明显减少，甚至许多医疗机构已弃用，而改用其他抗生素替代则往往达不到理想疗效且毒性增加。     

供精自愿者的心理顾虑及其对策

探讨我国人类精子库面临精源困难的原因，对浙江省计划生育科学技术研究所人类精子库1168名供精自愿者的心理状况进行分析，发现供精自愿者存在不同的心理顾虑，包括害怕资料泄密、对子代负有责任、子代近亲结婚、伤害身体以及影响学习或工作等，通过对供精自愿者心理顾虑因素及对策分析，以期解除他们有关的心理疑虑，提高供精员数量。     

安庆市肺结核病流行现状调查分析

目的了解安庆市肺结核病流行现状．为制定结核病控制对策提供科学依据。方法对1997～2001年结核病疫情报告和结核患者登记年报表进行分析。结果5年间，安庆市年均肺结核病报告发病率为61．15／10万，初治涂阳治愈率为90．31％，归口管理单位查痰率为91．05％。结论归口管理是提高本市结核病防治工作成效的重要措施。     

系统性健康教育对改善焦虑病人症状的影响

焦虑症是以焦虑、紧张、恐惧等情绪障碍为主,常伴有自主神经系统症状和运动性不安等[1].主要表现为惊恐发作和广泛性焦虑两种形式.随着社会的发展、生活节奏的加快、竞争的激烈、社会分工的多元化、婚姻的变故、考试的不理想等生活事件,使人们的精神压力大为增加[2].以往的传统求稳心态已落后于时代的发展,一种以焦虑情绪为主的神经症已成为一个时代的现代疾病.焦虑症的发展有逐年上升的趋势,已受到更多人的关注和注意.对焦虑症病人实施系统性的健康教育,降低焦虑症的复发率具有重要的意义.……     

“玉泉丸”颗粒剂的薄层色谱鉴别

古主“玉泉丸”改制成颗粒剂后，对处方中人参，黄芪，麦冬，乌梅，天花粉，葛根，甘草等七味中药的薄层色谱鉴别方法进行了探讨。     

181例病毒性心肌炎临床特点及心律失常类型

小儿病毒性心肌炎１８１例，其中暴发性及心源性休克型８例。其主要特点是：心脏增大（４３．４％），心律失常（９３．９％），其中早搏最多见（５９．９％）；传导阻滞（１５．５％）。作者认为应强调对Ⅱ°－Ⅱ型以上传导阻滞病情严重应给激素及临时起搏器抢救治疗；心电轴异常（３５．７％），亦是心肌炎的重要心电图改变之一。     

Chronic opioid treatment of neuroblastoma X dorsal root ganglion neuron hybrid F11 cells results in elevated GM1 ganglioside and cyclic adenosine monophosphate levels and onset of naloxone-evoked decreases in membrane K+ currents

Prolongation of the action potential duration of dorsal root ganglion (DRG) neurons by low (nM) concentrations of opioids occurs through activation of excitatory opioid receptors that are positively coupled via G_s regulatory protein to adenylate cyclase. Previous results suggested GM1 ganglioside to have an essential role in regulating this excitatory response, but not the inhibitory (APD-shortening) response to higher (μM) opioid concentrations. Furthermore, it was proposed that synthesis of GM1 is upregulated by prolonged activation of excitatory opioid receptor functions. To explore this possibility we have utilized cultures of hybrid F11 cells to carry out closely correlated electrophysiological and biochemical analyses of the effects of chronic opioid treatment on a homogeneous population of clonal cells which express many functions characteristic of DRG neurons. We show that chronic opioid exposure of F11 cells does, in fact, result in elevated levels of GM1 as well as cyclic adenosine monophosphate (AMP), concomitant with the onset of opioid excitatory supersensitivity as manifested by naloxone-evoked decreases in voltage-dependent membrane K⁺ currents. Such elevation of GM1 would be expected to enhance the efficacy of excitatory opioid receptor activation of the G_s/adenylate cyclase/cyclic AMP system, thereby providing a positive feedback mechanism that may account for the remarkable supersensitivity of chronic opioid-treated neurons to the excitatory effects of opioid agonists as well as antagonists. These in vitro findings may provide novel insights into the mechanisms underlying naloxone-precipitated withdrawal syndromes and opioid-induced hyperalgesia after chronic opiatf addiction in vivo. © 1995 Wiley-Liss, Inc.     

肿瘤转移相关蛋白在甲状腺乳头状腺癌伴淋巴结转移组织中的原位表达研究

利用免疫组化ＡＢＣ法，研究甲状腺乳头状腺癌，甲状腺腺瘤和正常甲状腺组织中的肿瘤转移相关基因蛋白ＣＤ４４ｖ６，ＥＧＦＲ，转移抑制基因ｎｍ２３－Ｈ１和抑癌基因ｐ５３蛋白的原位表达。结果发现ＣＤ４４ｖ６和ＥＧＦＲ表达上调与肿瘤转移密切相关（Ｐ＜０．０５，Ｐ＜０．０１），而ｎｍ２３－Ｈ１的表达与肿瘤转移抑制密切相关（Ｐ＜０．０１）。这提示肿瘤转移相关基因和转移抑制基因之间的表达失衡是甲状腺乳头状腺癌易发生转移的重要原因。     

红霉素对犬幽门括约肌压力影响及其与胃动素,生长抑素的关系

为探讨红霉素对犬幽门括约肌的作用及其机理，及其与血浆胃动素，生长抑素的关系。方法采用胃压力测量仪及放射免疫法，同步监测观察了１０条犬静脉滴ＥＭ前后和使用拮抗剂后的幽门压力、血浆ＭＴＬ，ＳＳ的变化，结论：ＥＭ具有增高犬胃幽门压力的作用，其作用机理除与血浆ＭＴＬ有着密切的关系外，血浆ＳＳ也可能共同参与了其调节机制。