限制性显示技术在制备丙型肝炎病毒分型芯片探针的应用性研究

目的探讨限制性显示(restriction display,RD)技术在制备丙型肝炎病毒(HCV)分型芯片探针的可行性.方法用限制性内切酶Sau3A I消化HCV1a、1b、2a亚型全长cDNA,所得的限制性内切酶片段与通用接头相连,通过10个选择性引物进行分组PCR,使得各片段得以扩增并分布于10个亚组中,经聚丙烯酰胺电泳(PAGE)结合银染法进行分离.切胶回收后作3次PCR,得到较纯净的HCV cDNA限制性片段.对这些片段做分子克隆,并测序鉴定.结果由HCV 3个亚型1a、2a、1b的全长cDNA得到66个大小相对均一(200～900bp)的限制性片段,平均每个亚型约22个.测序结果表明,属于HCV基因,可以作为HCV分型芯片的探针.结论RD技术能快速收集大量长度适宜、大小均一的病毒基因片段,适合于分型诊断基因芯片探针的收集.     

法国的新生儿死亡率:新生儿死亡证明书的应用

1997年法国开始使用新生儿死亡证明书。它提供了有关婴儿的死亡原因以及其出生情况和父母情况的详细资料。作者报道有关死亡证明书的研究新结果。方法:研究包括所有1999年在出生27d内死亡的新生儿(n=2036)。根据常规方法,主要根据国际疾病分类法,对死亡证明书进行分析。结果:87     

Pathogenic Mechanisms of Male Infertility Caused by Ureaplasma Urealyticum Infection——The Effect on Sperm Penetrating Capability

The effect of different U. urealyticum serovars on human sperm penetrating capabititty using zona-free hamster egg penetration assay was observed in this study.Two serovars of U. urealyticum(serotype 9 and 12) were incubated with hunran apermatozoa collected from normal fertile men at the concentration of 20-40 CCU/spermatozoon. It has been shown that fuzzy appearance was noticed at the tail part of infected spermatozoa,sperm viability and motility were declined dramatically after     

脑血管病合并癫痫133例临床特点与预后

脑血管病合并癫痫１３３例临床特点与预后高小飞，吴伟利，汤克仁（淄博市中心医院，２５５０３６）关键词脑血管病，癫痫，并发症文献报道脑血管病（ＣＶＤ）伴发癫痫率差异很大，从２．５％～３２％不等；伴发癫痫后对ＣＶＤ的预后也有严重影响。我院自１９８０年１月～...     

铂金属化合物的医学及环境学意义

1735年,西班牙的Antoni de Ulloa在平拖河金矿中首次发现了铂.铂金属因在自然界中含量甚微[1],故价格昂贵,和金、银一起被称为贵金属.铂是热和电的良好导体,熔、沸点都较高,具有良好的延展性,对光的反射率高,金属表面具有吸附氢气的特殊性能,还具有较好的抗腐蚀性和抗氧化性.     

明显威胁婴幼儿生命事件的病因:系统性回顾

目的:探讨当婴幼儿第一次出现明显威胁生命事件(ALTE)时最可能的诊断。方法:笔者检索了1966-2002年Medline、1980-2002年Embase以及1982-2002年Cinahl数据库的资料。为了进一步研究,笔者与有关专家进行联系。搜集了各种研究、回顾性文章和书籍。对外文研究进行翻译。如果文章对ALTE给出明确     

剖宫产手术留置导尿的时机选择

目的减轻剖宫产术留置导尿管孕妇的尿道刺激症状和紧张心理,提高服务质量。方法将200例剖宫产孕妇在硬膜外麻醉前后留置尿管时紧张、疼痛及满意度等比较。结果2组孕妇紧张、疼痛及满意度有显著性差异。结论硬膜外麻醉后插导尿管可明显减轻尿道刺激症、心理压力、提高满意度。     

子宫肌瘤经子宫动脉栓塞术治疗后患者满意度和生活质量调查

目的:评估子宫肌瘤患者经子宫动脉栓塞术(UAE)治疗后临床症状严重程度、健康相关生活质量(HRQOL)的变化及对治疗满意度的调查。设计:对1998～2002年80例经UAE治疗的子宫肌瘤患者进行问卷调查,了解治疗的有效性、治疗前后的临床症状严重程度及HRQOL的变化。主要观察指标:UAE治疗后症状严重程度及HRQOL评分的变化。次要观察指标:患者满意度及子宫体积缩小程度。结果:64例(80.0%)患者完成了问卷,调查时间为UAE后平均32.1月(范围:57.5～6月)。UAE后子宫体积平均缩小26.3%(95%CI19.6～33.0),79例患者中17例(21.5%)于术后平均18.6月…     

A Phase III, Double-blind, Placebo-controlled, Multicenter Study on the Efficacy of Recombinant Human Antithrombin in Heparin-resistant Patients Scheduled to Undergo Cardiac Surgery Necessitating Cardiopulmonary Bypass

Background: The study evaluated the efficacy of recombinant human antithrombin (rhAT) for restoring heparin responsiveness in heparin resistant patients undergoing cardiac surgery.

Methods: This was a multicenter, randomized, double-blind, placebo-controlled study in heparin-resistant patients undergoing cardiac surgery with cardiopulmonary bypass. Heparin resistance was diagnosed when the activated clotting time was less than 480 s after 400 U/kg heparin. Fifty-four heparin-resistant patients were randomized. One cohort received 75 U/kg rhAT, and the other received normal saline. If the activated clotting time remained less than 480 s, this was considered treatment failure, and 2 units fresh frozen plasma was transfused. Patients were monitored for adverse events.

Results: Only 19% of patients in the rhAT group received fresh frozen plasma, compared with 81% of patients in the placebo group (P < 0.001). During their hospitalization, 48% of patients in the rhAT group received fresh frozen plasma, compared with 85% of patients in the placebo group (P = 0.009). Patients in the placebo group required higher heparin doses (P < 0.005) for anticoagulation. There was no increase in serious adverse events associated with rhAT. There was increased blood loss 12 h postoperatively (P = 0.05) with a trend toward increased 24-h bleeding in the rhAT group (P = 0.06). There was no difference between the groups in blood and platelet transfusions.     

三氧化二砷治疗复发急性早幼粒细胞白血病58例

1996-2004年9月，我们应用三氧化二砷共治疗58例复发急性早幼粒细胞白血病(APL)，现报道如下。