新型硬化剂的动物实验与临床应用

目的；寻找更有效的硬化剂治疗管静脉曲张破裂出血，方法；配制一种新型硬化剂 成分为１．５％孟氏液７０ｍｌ，２０％利多卡因２０ｍｌ，红霉素０．５ｇ及注射用水１０ｍｌ，将这种经剂注射到兔耳的血管内，还应用于９便肝硬化食管静脉曲张破裂出血病人，并设对照组。结果；新型硬化剂局部上血比５％鱼肝油酸钠和消痔灵的效果好，临上血效果与１％乙氧硬化醇相同，且对动物肝、肾无损害。结论；新型硬化剂具有安全、有效、副作用小     

肝动脉插管灌注化疗及栓塞术治疗中晚期原发性肝癌48例报告

肝动脉插管灌注化疗及栓塞术治疗中晚期原发性肝癌４８例报告南阳市中心医院肿瘤科张成辉，仝运科南阳市中心医院放射科曾宪强，王，张涛结导管肝动脉灌注化疗（ＴＡＩ）及栓塞术（ＴＡＥ）作为中晚期原发性肝癌（下简称肝癌）首选的故息疗法已在国内广泛开展。我院自１９...     

川北地区人血清β_2－MG，α_1－MGTHP正常值的RIA测定

本文采用放射免疫分析法（ＲＩＡ）测定了８０例健康人血清β＿２－ＭＧ、α＿１－ＭＧ、ＴＨＰ的正常含量，其测定结果比其它地区报导的正常值偏低，对川北地区肾功能障碍患者的早期诊断有一定的参考价值。     

紫花合掌消的生药学研究

中药白薇的正品来源为萝摩科植物白薇Ｃｙｎａｎｃｈｕｍ ａｔｒａｔｕｍ Ｂｇｅ．或蔓生白薇Ｃｙｎａｎｔｕｍ ｖｅｒｓｉｃｏｌｏｒ Ｂｇｅ．的干燥根及根茎。由于用药习惯不同，全国各地使用白薇的品种略有不同，湖北省以同科植物紫花合掌消Ｃｙｎａｎｔｕｍ ａｍｐｌｅｘｉｃａｕｌｅ ｖｅｒ．ｃａｓｔｕｎｌｕｍ为白薇的主要商品来源。清吴其浚著《植物名实图考》对合掌消的植物形态作了详细描述：“……秋时梢头发细枝，     

孕妇传染病血清流行病学监测报告

孕妇传染病血清流行病学监测报告郝宗宇，张涛，吕家锐，张爱梅，付玉生，武林，姜艳玲，王华钦，孟淑霞，王广科，徐曼莉，郝应平，芦素莲，赵红英，赵妮出生缺陷是世界各国政府和人民都极为重视的一大社会问题，优生优育是提高国民素质的重要保证。但许多病原生物因素无...     

静脉复合加利多卡因局部麻醉用于小儿气管异物取出术

2001年12月-2003年12月，笔者选择静脉复合麻醉加利多卡因局部麻醉，用于57例小儿气管异物取出术，取得满意效果，现报告如下。     

大鼠脂肪干细胞复合胶原-壳聚糖-硫酸软骨素三维支架构建组织工程软骨

Objective To evaluate the character of the collagen-chitosan-chondroitin sulfate scaffold seeded with rat adipose tissue-derived stromal cells. Methods A dipose tissue were harvested from 6 weeks old Wistar rats and the stromal cells were harvested by type Ⅰ collagenase and then cultured in vitro. Type Ⅰ collagen was fully mixed with chitosan, freeze-dried and cross-linked with chondroitin sulfate, then freeze-dried again and sterilized by ethylene oxide. The pore diameter, water content, porosity of the scaffold were tested. The adipose tissue-derived stromal cells were digested, seeded into the plates, scaffold, and cen-trifuged into pellet, and then induced into cartilage. MTT detection for cell proliferation was done. After 3 weeks, the cell morphology, and cell proliferation and adhesion were observed, and chondrngenic differenti-ation was also analyzed. Results The pore diameter, water content, porosity tested for the scaffold showed an appropriate form. Cell proliferation showed faster in the scaffold and pellet culture system after 5 day, there was still cell proliferation in the scaffold system after 14 days but no obvious changes in the pellet cul-ture system; ceils on the scaffold proliferated densely showed by histological staining, but there was a scaf-fold structure residues in the inner layer. The finding of type Ⅱ immunohistochemistry stain showed that cells express strong positive for type Ⅱ collagen in the scaffold and pellet culture system whereas it was weakly positive in the plate culture system; the specific mRNA for cartilage, type Ⅱ collagen, aggrecan and SOX-9 were expressed in all three systems showed by RT-PCR, but type X collagen was expressed continu-ously in the plate culture system and expressed after 21 days in the pellet culture system, whereas it was not detected in the collagen-chitosan-chondroitin sulfate scaffold system. Conclusion The parameters of the collagen-chitosan-chondroitin sulfate scaffold were suitable in our study. The results suggested that it can promote the adipose tissue-derived stromal cells proliferation and chondrogenic differentiation better than the plate and pellet culture systems and maintain the phenotype of chondrocytes well; it is the optimal choice for cartilage tissue engineering in the future.     

硬膜外腔注射生理盐水对剖宫产术患者腰麻效果的影响

目的探讨硬膜外腔注射生理盐水对剖宫产术患者腰麻效果的影响。方法择期行子宫下段剖宫产术患者60例,年龄24～30岁,体重59～73 kg,随机分为2组,每组30例,A组蛛网膜下腔注射规定剂量的0.75%布比卡因后硬膜外腔注射生理盐水5 ml;B组蛛网膜下腔注射0.75%布比卡因。按序贯法进行试验,设定布比卡因的起始剂量为9 mg,剂量梯度为1.5 mg,若上一例有效,则下一例递减一个剂量梯度,若无效则下一例递增一个剂量梯度,蛛网膜下腔阻滞有效的标准为注射布比卡因后20 min内阻滞上平面达T5。采用概率单位法计算ED50。结果A组布比卡因的ED50（5.8 mg）低于B组（8.1 mg）,两组比值为0.72,95%置信区间为0.27～0.98,区间范围不包括1,差异有统计学意义（P〈0.05）。结论硬膜外腔注射生理盐水可增强剖宫产术患者腰麻的效果。     

胆系感染中毒性休克

胆系感染中毒性休克新泰市中医院（２７１２００）韩庆琏，张涛，王渊患者女，５５岁。１９７４年春以胆系感染中毒性休克急症入镇医院外科治疗，给予抗生素、升压药物、维生素、输液等对症治疗３天，病情无好转，外科无手术指征而请中医会诊。患者呈中度昏迷状态，全身黄...     

原发性中枢神经系统恶性淋巴瘤MR表现及其病理学基础

目的 研究原发性中枢神经系统恶性淋巴瘤(PCNSL)的MR表现及其病理学基础。方法 分析13例手术病理证实的原发性中枢神经系统恶性淋巴瘤的临床病理及MR表现。结果 13例中单发肿瘤4例，多发肿瘤9例，共计36个病灶。13例病变均累及幕上，其中8例病灶位于深部脑白质近脑室旁。肿瘤平均最大径为3．2cm。T1WI略低信号28个，T2WI等信号24个。28个病灶呈均匀强化。肿瘤水肿及占位效应相对较轻。2例PCNSL行MR动态增强扫描，早期强化均不明显，时间-信号强度曲线呈缓慢上升型。病理上肿瘤细胞弥漫分布，瘤细胞大小较一致，细胞质少，细胞核大，染色质颗粒粗，可见瘤细胞围绕血管呈袖套样浸润，少见明显的出血及片状坏死，未见钙化，病理均为非霍奇金淋巴瘤。结论 原发性中枢神经系统恶性淋巴瘤的病理基础决定其MR增强形态、占位程度以及肿瘤发生部位具有一定特征，运用不同的MR影像学检查方法和技术，在多数情况下可以做出术前正确诊断。