基因工程重组人超氧化物歧化酶抗炎作用的研究

通过角叉菜胶诱发大鼠足水肿这一经典炎症模型,观察了基因工程重组人铜锌超氧化物歧化酶(Recombined Human Superoxide Dismutase;rh SOD)对非感染性炎症的抗炎作用,证实了静脉注射 rh SOD(7000 U/kg～10 000 U/kg)对大鼠足肿胀有明显抑制作用,实验组与对照组间有显著性差异(P<0.001),增加剂量和给药次数可提高抗炎效果.该结果为进一步探讨rh SOD抗放射线损伤的机制奠定了基础.     

Effects of derivatives of human glycophorin A and their antibodies on the recognition and invasion of Plasmodium falciparum into erythrocytes

Electrophoresis-purified human glycophorin A(GPA)was used to produce its derivatives:(1)separation and purification of glycopeptides from GPA were performed after trypsin-digestion:(2)prepanation of GPA antibody and GPA glycopeptide antibody;(3)preparation of deglycosylatedGPA(dGPA);(4)incorporating GPA or dGPA into human RBC membrane lipids to form twokinds of liposomes.The products described above were used to test Plasmodim falciparumFCC-1/HN merozoites for their ability to invade human erythrocytes.It was found that GPA-liposomes were able to bind with merozoites and dGPA-liposomes had a negative reaction.GPA,GPA glycopeptide,GPA antibody,GPA glycopeptide antibody and GPA-liposome all had the effectto hinder the invasion of merozoites into human erythrocyte,whereas dGPA-liposome had no suchan effect.     

rhCu/ZnSOD对γ-线照射小鼠抗氧化酶活性的影响

γ线照射可导致小鼠细胞内外超氧化物歧化酶、谷胱甘肽过氧化物酶以及过氧化氢酶活力明显降低。分别于照射前后，单纯照射前及单纯照射后给小鼠腹腔注射基因工程重组人铜锌超氧化物歧化酶，给药组小鼠细胞内外各抗氧化酶活力的降低程度明显减轻；平均活存时间和死亡率亦与对照组有显著差异。     

抗人TNF-α单抗基因的克隆及鉴定

目的克隆抗人ＴＮＦ－α鼠单抗得可变区基因以构建人－鼠嵌合抗体表达载体。方法采用ＲＴ－ＰＣＲ技术,以前导肽序列的引物从１个分泌抗人ＴＮＦ－α的鼠单抗杂交瘤细胞系中克隆抗体轻链、重链可变区基因（Ｖκ,ＶＨ）,在大肠杆菌中表达Ｆａｂ段核实其功能活性。结果分别得到了２个Ｖκ和２个ＶＨ基因。ＤＮＡ序列测定表明,其中１个轻链可变区基因为骨髓瘤细胞系中固有的无功能基因。１个重链可变区基因经原核系统表达测活表明无抗体活性。另一个轻链和重链可变区基因的成熟蛋白编码部分与从第一骨架区引物所克隆的、可在大肠杆菌表达出抗体活性的Ｖκ、ＶＨ序列相符。将该轻链、重链基因分别克隆到了人－鼠嵌合轻链、重链表达载体中。结论通过原核表达系统核实,获得了抗ＴＮＦ－α单抗的可变区基因